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Clinical and diagnostic laboratory immunology1996; 3(1); 47-50; doi: 10.1128/cdli.3.1.47-50.1996

Differences between Taylorella equigenitalis strains in their invasion of and replication in cultured cells.

Abstract: The ability of Taylorella equigenitalis, the causative agent of contagious equine metritis, to invade and replicate in equine derm cells was studied. The kinetics of invasion and replication were determined for four T. equigenitalis strains. On the basis of these experiments, a simpler assay in which the invasive as well as the replicative properties of a particular strain could be determined was developed. This assay was used to characterize 32 strains, which had previously been typed by field inversion gel electrophoresis of genomic restriction fragments. The invasiveness of T. equigenitalis strains ranged from 3 to 0.015 bacteria per cell and seemed to be associated with the contagiousness of the infection. The replication index (number of intracellular bacteria per cell at 24 h after inoculation divided by the number of intracellular bacteria per cell at 4 h after inoculation) varied from 1 to 857 and seemed to be associated with the severity of the symptoms of contagious equine metritis. There was no association between the invasiveness and the replication index of the strains, nor was there an association of invasion and replication with field inversion gel electrophoresis grouping.
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Publication Date: 1996-01-01 PubMed ID: 8770503PubMed Central: PMC170246DOI: 10.1128/cdli.3.1.47-50.1996Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on studying the different levels of invasion and replication by strains of Taylorella equigenitalis, a bacteria causing contagious equine metritis, in equine derm cells. Strains’ invasiveness seems connected to the contagiousness of the infection while the bacteria’s rate of replication might influence the severity of the disease symptoms.

Objective and Methodology

  • The study aimed to investigate differences in the invasion and replication capabilities of various Taylorella equigenitalis strains. These strains are responsible for contagious equine metritis, a sexually transmitted disease in horses primarily affecting mares.
  • The researchers determined the kinetics of invasion and replication for four different strains- through the use of an assay they developed. This assay had the ability to both measure a strain’s invasiveness and its replication rate.
  • The assay was then used to characterize 32 strains that had previously been categorized using field inversion gel electrophoresis of genomic restriction fragments.

Findings

  • The study found a wide range in the invasive properties of T. equigenitalis strains, with values spanning from 0.015 to 3 bacteria per cell.
  • The researchers observed an apparent correlation between a strain’s invasiveness and the contagiousness of the infection it caused.
  • The replication index is defined as the number of intracellular bacteria per cell at 24 hours after inoculation divided by the number at 4 hours after inoculation. This variable also exhibited varied values, from 1 to 857.
  • The research suggested a potential link between the replication index and the severity of contagious equine metritis symptoms.

Lack of Association

  • Despite exploring two defining features of the strains—invasiveness and replication rate—the study found no relationship between these two characteristics.
  • Moreover, there was no association of strain invasion or replication capabilities with their grouping by field inversion gel electrophoresis- indicating that this widely-used method for typing bacteria strains may not be indicative of the strains’ biological behavior in terms of invasiveness and replication rate.

Cite This Article

APA
Bleumink-Pluym NM, ter Laak EA, Houwers DJ, van der Zeijst BA. (1996). Differences between Taylorella equigenitalis strains in their invasion of and replication in cultured cells. Clin Diagn Lab Immunol, 3(1), 47-50. https://doi.org/10.1128/cdli.3.1.47-50.1996

Publication

Clinical and diagnostic laboratory immunology
ISSN: 1071-412X
NlmUniqueID: 9421292
Country: United States
Language: English
Volume: 3
Issue: 1
Pages: 47-50

Researcher Affiliations

Bleumink-Pluym, N M
  • Department of Bacteriology, The Netherlands.
ter Laak, E A
    Houwers, D J
      van der Zeijst, B A

        MeSH Terms

        • Animals
        • Bacterial Adhesion
        • Cell Division
        • Cells, Cultured
        • Endometritis / etiology
        • Endometritis / veterinary
        • Female
        • Haemophilus / growth & development
        • Haemophilus / pathogenicity
        • Haemophilus / physiology
        • Horse Diseases / etiology
        • Horses
        • Sexually Transmitted Diseases / etiology
        • Sexually Transmitted Diseases / veterinary
        • Species Specificity

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        Citations

        This article has been cited 4 times.
        1. Grabatin M, Fux R, Zablotski Y, Goehring LS, Witte TS. Taylorella equigenitalis in Icelandic intact males compared with other horse breeds using natural cover. Equine Vet J 2025 Mar;57(2):441-448.
          doi: 10.1111/evj.14121pubmed: 39031711google scholar: lookup
        2. Hicks J, Stuber T, Lantz K, Erdman M, Robbe-Austerman S, Huang X. Genomic diversity of Taylorella equigenitalis introduced into the United States from 1978 to 2012. PLoS One 2018;13(3):e0194253.
          doi: 10.1371/journal.pone.0194253pubmed: 29584782google scholar: lookup
        3. Allombert J, Vianney A, Laugier C, Petry S, Hébert L. Survival of taylorellae in the environmental amoeba Acanthamoeba castellanii. BMC Microbiol 2014 Mar 19;14:69.
          doi: 10.1186/1471-2180-14-69pubmed: 24641089google scholar: lookup
        4. Hébert L, Moumen B, Pons N, Duquesne F, Breuil MF, Goux D, Batto JM, Laugier C, Renault P, Petry S. Genomic characterization of the Taylorella genus. PLoS One 2012;7(1):e29953.
          doi: 10.1371/journal.pone.0029953pubmed: 22235352google scholar: lookup
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