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Lipids1993; 28(11); 999-1003; doi: 10.1007/BF02537121

Different resistance of mammalian red blood cells to hemolysis by bile salts.

Abstract: To evaluate why hemolysis of red blood cells (RBC) by bile acids varies in different mammalian species, we determined the mean corpuscular volume (MCV), lipid content and the concentrations of the conjugates of deoxycholate and of NaCl inducing 50% hemolysis of RBC from healthy humans, pigs, horses, cows, sheep and jaundiced humans. A volume of 0.05 mL of washed RBC at 1% hematocrit, which has the same lipid content but different phospholipid composition and number of erythrocytes (owing to the variable MCV), was incubated in taurodeoxycholate (TDC) solution (0-5 mM) to determine the TDC concentration inducing 50% hemolysis (TDC50). The TDC50 was highest in RBC of sheep and decreased within the series sheep > pig > cow > horse > healthy human > jaundiced human, which have generally increasing MCV. The osmotic resistance followed an inverse order, with jaundiced human > healthy human > horse > cow > pig > sheep. Although we found no correlation between the TDC50 and phospholipid composition of the erythrocytes tested, the extent of bile salt-induced hemolysis seemed to depend on both the MCV and the number of erythrocytes in the incubation medium.
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Publication Date: 1993-11-01 PubMed ID: 8277831DOI: 10.1007/BF02537121Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates why the rupture of red blood cells by bile acids differs among various mammalian species. It found that the extent of bile salt-induced hemolysis depends on both the mean corpuscular volume and the number of red blood cells in the study medium.

Objective of the Research

  • The study was designed to understand why bile acids cause varying degrees of hemolysis, or rupture of red blood cells, in different mammalian species.
  • The scientists concentrated on determining the mean corpuscular volume (MCV), lipid content and concentrations of specific bile salt conjugates in red blood cells from healthy humans, pigs, horses, cows, sheep and jaundiced humans, to draw connections between these elements and the degree of hemolysis each species’ RBCs demonstrated.

Methods and Procedures

  • The investigators used 0.05 mL of washed red blood cells at 1% hematocrit (the ratio of the volume of red blood cells to the total volume of blood), with the same lipid content but different phospholipid compositions and varying numbers of red blood cells due to the different MCV. These RBCs were incubated in a taurodeoxycholate (TDC) solution of differing concentrations.
  • The experiment was designed to determine the concentration of TDC needed to induce 50% hemolysis (TDC50).

Results of the Study

  • Findings showed that the TDC50 was highest in sheep RBCs and decreased in this order: sheep > pig > cow > horse > healthy human > jaundiced human. These species also demonstrated an increasing trend in MCV.
  • The resistance to hemolysis by osmotic pressure was the opposite, with jaundiced human RBCs showing the highest resistance, followed by healthy human RBCs, then horse, cow, pig and finally sheep.
  • Despite the study, scientists found no correlation between the TDC50 and the phospholipid composition of the erythrocytes examined.
  • The degree of hemolysis induced by bile salt seemed to be dependent on both the MCV and the number of red blood cells subjected to the experimental conditions.

Cite This Article

APA
Salvioli G, Gaetti E, Panini R, Lugli R, Pradelli JM. (1993). Different resistance of mammalian red blood cells to hemolysis by bile salts. Lipids, 28(11), 999-1003. https://doi.org/10.1007/BF02537121

Publication

Lipids
ISSN: 0024-4201
NlmUniqueID: 0060450
Country: United States
Language: English
Volume: 28
Issue: 11
Pages: 999-1003

Researcher Affiliations

Salvioli, G
  • University of Modena, Ospedale Estense, Italy.
Gaetti, E
    Panini, R
      Lugli, R
        Pradelli, J M

          MeSH Terms

          • Animals
          • Bile Acids and Salts / pharmacology
          • Cholestasis
          • Dose-Response Relationship, Drug
          • Erythrocyte Membrane / chemistry
          • Erythrocyte Membrane / drug effects
          • Erythrocyte Volume
          • Glycodeoxycholic Acid / pharmacology
          • Hemolysis / drug effects
          • Horses / physiology
          • Humans
          • Osmotic Fragility / physiology
          • Phospholipids / analysis
          • Ruminants / physiology
          • Sodium Chloride / pharmacology
          • Sphingomyelins / analysis
          • Taurodeoxycholic Acid / pharmacology

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          Citations

          This article has been cited 2 times.
          1. Dennison SR, Phoenix DA. Susceptibility of sheep, human, and pig erythrocytes to haemolysis by the antimicrobial peptide Modelin 5. Eur Biophys J 2014 Sep;43(8-9):423-32.
            doi: 10.1007/s00249-014-0974-9pubmed: 25030320google scholar: lookup
          2. Kurmangaliyeva S, Baktikulova K, Tkachenko A, Seitkhanova B, Tryfonyuk L, Rakhimzhanova F, Yussupov R, Kurmangaliyev K. Eryptosis in Liver Diseases: Contribution to Anemia and Hypercoagulation. Med Sci (Basel) 2025 Aug 12;13(3).
            doi: 10.3390/medsci13030125pubmed: 40843747google scholar: lookup
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