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Veterinary and comparative oncology2016; 15(3); 764-774; doi: 10.1111/vco.12216

Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells.

Abstract: Bovine papillomavirus (BPV) types 1 and 2 play an important role in the pathogenesis of equine sarcoids (ES), the most common cutaneous tumour affecting horses. MicroRNAs (miRNAs), small non-coding RNAs that regulate essential biological and cellular processes, have been found dysregulated in a wide range of tumours. The aim of this study was to identify miRNAs associated with ES. Differential expression of miRNAs was assessed in control equine fibroblasts (EqPalFs) and EqPalFs transformed with the BPV-1 genome (S6-2 cells). Using a commercially available miRNA microarray, 492 mature miRNAs were interrogated. In total, 206 mature miRNAs were differentially expressed in EqPalFs compared with S6-2 cells. Aberrant expression of these miRNAs in S6-2 cells can be attributed to the presence of BPV-1 genomes. Furthermore, we confirm the presence of 124 miRNAs previously computationally predicted in the horse. Our data supports the involvement of miRNAs in the pathogenesis of ES.
© 2016 John Wiley & Sons Ltd.
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Publication Date: 2016-04-04 PubMed ID: 27039895DOI: 10.1111/vco.12216Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper is about the identification of certain microRNAs that are expressed differently in equine cells transformed by bovine papillomavirus type 1, which is known to contribute to equine sarcoids, a common skin tumor in horses.

Research Background

  • Bovine papillomavirus types 1 and 2 (BPV-1 and BPV-2) are agents evidently associated with the development of equine sarcoids (ES). ES is a prevalent skin tumor affecting horses.
  • MicroRNAs (miRNAs) are small, non-coding RNAs with significant biological roles. They regulate many basic biological and cellular processes and have been found to be expressed differently in various types of tumors.
  • The goal of this research was to identify particular miRNAs linked with ES by comparing their expression in normal horse cells and horse cells transformed with BPV-1.

Research Methodology

  • In this study, the researchers sampled normal equine fibroblasts (EqPalFs) and EqPalFs which had been transformed using the BPV-1 genome, referred to as S6-2 cells.
  • A miRNA microarray, which can analyze the expression of many miRNAs simultaneously, was utilized. In this research, 492 mature miRNAs were evaluated.

Research Findings

  • The research findings unveiled that 206 miRNAs were differentially expressed. These variations in expression were seen when comparing the normal EqPalFs and S6-2 cells.
  • The abnormal expression of these miRNAs in S6-2 cells was connected to the presence of BPV-1 genomes. Thus, the transformation of the cells with the BPV-1 genome appears to change the way the cells express these miRNAs.
  • The study also affirmed the existence of 124 miRNAs that had previously been predicted in horses via computational predictions.

Research Significance

  • This research emphasizes the role of miRNAs in the development of ES. It provides a valuable understanding of the links between BPV-1, miRNA differential expression, and the development of ES.
  • The findings of this study could potentially be leveraged for therapeutic targets in the treatment of ES in the future.

Cite This Article

APA
Terron-Canedo N, Weir W, Nicolson L, Britton C, Nasir L. (2016). Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells. Vet Comp Oncol, 15(3), 764-774. https://doi.org/10.1111/vco.12216

Publication

Veterinary and comparative oncology
ISSN: 1476-5829
NlmUniqueID: 101185242
Country: England
Language: English
Volume: 15
Issue: 3
Pages: 764-774

Researcher Affiliations

Terron-Canedo, N
  • MRC - University of Glasgow Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Weir, W
  • Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Nicolson, L
  • School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Britton, C
  • Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Nasir, L
  • School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.

MeSH Terms

  • Animals
  • Bovine papillomavirus 1
  • Cell Transformation, Viral
  • Fibroblasts / metabolism
  • Fibroblasts / virology
  • Horse Diseases / metabolism
  • Horses
  • MicroRNAs / metabolism
  • Papillomavirus Infections / metabolism
  • Papillomavirus Infections / veterinary
  • Papillomavirus Infections / virology
  • Real-Time Polymerase Chain Reaction / veterinary
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / veterinary
  • Skin Neoplasms / virology

Citations

This article has been cited 7 times.
  1. Hamza E, Cosandey J, Gerber V, Koch C, Unger L. The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids. Vet Res Commun 2023 Jan;47(1):87-98.
    doi: 10.1007/s11259-022-09930-7pubmed: 35484337google scholar: lookup
  2. Cosandey J, Hamza E, Gerber V, Ramseyer A, Leeb T, Jagannathan V, Blaszczyk K, Unger L. Diagnostic and prognostic potential of eight whole blood microRNAs for equine sarcoid disease. PLoS One 2021;16(12):e0261076.
    doi: 10.1371/journal.pone.0261076pubmed: 34941894google scholar: lookup
  3. Unger L, Abril C, Gerber V, Jagannathan V, Koch C, Hamza E. Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys. J Vet Intern Med 2021 Jan;35(1):610-619.
    doi: 10.1111/jvim.16027pubmed: 33415768google scholar: lookup
  4. Pang F, Chen Z, Wang C, Zhang M, Zhang Z, Yang X, Zheng Y, Liu A, Cheng Y, Chen J, Li B, Du L, Wang F. Comprehensive analysis of differentially expressed microRNAs and mRNAs in MDBK cells expressing bovine papillomavirus E5 oncogene. PeerJ 2019;7:e8098.
    doi: 10.7717/peerj.8098pubmed: 31772843google scholar: lookup
  5. Pang F, Zhang M, Li G, Zhang Z, Huang H, Li B, Wang C, Yang X, Zheng Y, An Q, Zhang L, Du L, Wang F. Integrated mRNA and miRNA profiling in NIH/3T3 cells in response to bovine papillomavirus E6 gene expression. PeerJ 2019;7:e7442.
    doi: 10.7717/peerj.7442pubmed: 31396463google scholar: lookup
  6. Unger L, Jagannathan V, Pacholewska A, Leeb T, Gerber V. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression. J Vet Intern Med 2019 Jan;33(1):241-250.
    doi: 10.1111/jvim.15375pubmed: 30506726google scholar: lookup
  7. Beermann A, Hamza E, Reinhard S, Koch C, Oberhänsli T, Unger L. Selected microRNAs as biomarkers in sarcoid-affected horses under immunotherapy with a mistletoe extract. J Vet Diagn Invest 2026 Jan;38(1):33-40.
    doi: 10.1177/10406387251362820pubmed: 41039872google scholar: lookup
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