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Home / Equine Research Bank / Domest Anim Endocrinol / Differential miRNA expression between equine ovulatory and a...
Domestic animal endocrinology2013; 45(3); 122-125; doi: 10.1016/j.domaniend.2013.06.006

Differential miRNA expression between equine ovulatory and anovulatory follicles.

Abstract: Relatively little is known about the physiological roles of microRNAs (miRNAs) during follicular development. Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian follicle development. The objective of this study was to gain insight on the involvement of these miRNAs during follicle maturation. Follicular fluid was aspirated from dominant follicles (>32 mm) during the ovulatory season (July to October) and the anovulatory season (January to March) in each of 5 mares, and the levels of steroids, IGF1, and miRNAs were analyzed by immunoassays and quantitative PCR. Levels of progesterone, testosterone, and IGF1 were lower (P ≤ 0.05) in anovulatory than in ovulatory follicles. Relative to ovulatory follicles, anovulatory follicles had higher (P < 0.05) mean levels of miR-21, miR-23b, miR-378, and miR-202 and tended to have higher (P = 0.06) levels of miR-145. Levels of miR-224 and miR-383 could not be detected in follicular fluid. These novel results indicate a physiological association between increases in follicular miRNA levels and seasonal anovulation in mares; further studies should elucidate the precise involvement of miR-21, miR-23b, miR-145, miR-378, and miR-202 in follicle maturation in the mare.
Copyright © 2013 Elsevier Inc. All rights reserved.
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Publication Date: 2013-07-27 PubMed ID: 23932580DOI: 10.1016/j.domaniend.2013.06.006Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the roles of specific microRNAs in the development of ovarian follicles in horses. It reveals higher levels of certain microRNAs in anovulatory follicles compared to ovulatory ones, linking a rise in follicular microRNA levels with seasonal anovulation.

Objective and Methodology

  • The primary goal of the research was to enhance understanding of the role of several microRNAs, specifically miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, during follicle growth and maturation.
  • Testing involved collecting follicular fluid from dominant ovulatory and anovulatory follicles of horses during different seasons. This approach allowed the researchers to track seasonal variation in ovulation.

Results and Findings

  • The research discovered that levels of progesterone, testosterone, and IGF1 were significantly lower in anovulatory follicles compared to ovulatory ones.
  • Moreover, the study found increased mean levels of multiple microRNAs (miR-21, miR-23b, miR-378, and miR-202) in anovulatory follicles. They also observed a tendency for higher levels of miR-145.
  • However, the researchers weren’t able to detect miR-224 and miR-383 in the follicular fluid, highlighting more exploration is required around these particular microRNAs.

Implications and Future Study

  • The research adds to growing evidence of a correlation between increased follicular microRNA levels and seasonal anovulation in mares. These findings open up new avenues of exploration in ovarian follicle development and maturity.
  • This outcome implies that certain microRNAs could potentially serve as markers for anovulation, and could even provide targets for therapeutic intervention in disorders of ovulation.
  • More extensive research is required to confirm the specific roles of miR-21, miR-23b, miR-145, miR-378, and miR-202 in follicle maturation.

Cite This Article

APA
Donadeu FX, Schauer SN. (2013). Differential miRNA expression between equine ovulatory and anovulatory follicles. Domest Anim Endocrinol, 45(3), 122-125. https://doi.org/10.1016/j.domaniend.2013.06.006

Publication

Domestic animal endocrinology
ISSN: 1879-0054
NlmUniqueID: 8505191
Country: United States
Language: English
Volume: 45
Issue: 3
Pages: 122-125
PII: S0739-7240(13)00088-X

Researcher Affiliations

Donadeu, F X
  • The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, EH25 9RG, Midlothian, UK. Electronic address: xavier.donadeu@roslin.ed.ac.uk.
Schauer, S N

    MeSH Terms

    • Animals
    • Female
    • Follicular Fluid / chemistry
    • Horses / physiology
    • Immunoassay
    • Insulin-Like Growth Factor I / analysis
    • MicroRNAs / analysis
    • MicroRNAs / physiology
    • Ovarian Follicle / chemistry
    • Ovarian Follicle / metabolism
    • Ovarian Follicle / physiology
    • Ovulation / physiology
    • Progesterone / analysis
    • Seasons
    • Testosterone / analysis

    Grant Funding

    • Biotechnology and Biological Sciences Research Council

    Citations

    This article has been cited 17 times.
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