FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Science / Differentiation of respiratory and abortigenic isolates of e...
Science (New York, N.Y.)1981; 214(4520); 562-564; doi: 10.1126/science.6270790

Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases.

Abstract: Viruses classified by immunologic criteria as equine herpesvirus 1 cause respiratory disease and abortion in horses. Restriction endonuclease analyses of the DNA's of viruses from animals with respiratory disease and from aborted fetuses show that the patterns for respiratory viruses, while similar to each other, are entirely different from the patterns for fetal viruses. It is therefore proposed that the DNA restriction endonuclease patterns of fetal and respiratory viruses analyzed in this study be designated as prototypic of equine herpesvirus 1 and 4, respectively.
Get Full Text
Publication Date: 1981-10-30 PubMed ID: 6270790DOI: 10.1126/science.6270790Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article primarily deals with the use of restriction endonuclease analyses to differentiate between respiratory and abortigenic variants of the equine herpesvirus 1 (EHV-1), which affects horses.

Understanding EHV-1

  • Equine herpesvirus 1 (EHV-1) is a common virus causing respiratory disease and abortion in horses. Despite being classified as the same virus, the disease it manifests can vary widely based on its strain.
  • The virus affects horses around the globe, causing significant economic losses within the equine industry. Hence, understanding its different variants is vital for efficient disease management.

Role of Restriction Endonucleases in the Study

  • The observations in the research were made possible through restriction endonuclease analyses of EHV-1 DNA from different animal hosts.
  • Restriction endonucleases are enzymes that can cut DNA strands at specific locations called restriction sites. By identifying these sites, scientists can create detailed ‘maps’ or patterns of DNA – a method sophisticatedly used in genetic engineering and genetics research.

Significant Findings

  • Through the restriction endonuclease analyses of the EHV-1 DNA, the research determined that while the DNA patterns of respiratory disease-causing variants are similar, they are entirely different from the abortigenic (abortion-causing) variants.
  • This finding suggests that there may be distinct genetic variations between abortigenic and respiratory forms of EHV-1, likely contributing to the differing disease manifestations.

Proposed Designations

  • Based on the significant differences revealed in the restriction endonuclease patterns, the authors conclude by proposing that these patterns define two different ‘prototypes’ within EHV-1.
  • The DNA pattern representative of abortigenic variants is proposed as the prototype for EHV-1, while the pattern of the respiratory disease-causing variants is suggested as the prototype for a new classification, EHV-4.

Implications of the Research

  • The proposed distinction between EHV-1 and EHV-4 may be crucial in future diagnostic, prevention, and treatment strategies given the differing health impacts of each variant.
  • Furthermore, this research underpins the significance of employing genetic methodologies such as restriction endonuclease analyses in virus study, highlighting their potential to reveal important distinctions within seemingly identical diseases.

Cite This Article

APA
Studdert MJ, Simpson T, Roizman B. (1981). Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science, 214(4520), 562-564. https://doi.org/10.1126/science.6270790

Publication

Science (New York, N.Y.)
ISSN: 0036-8075
NlmUniqueID: 0404511
Country: United States
Language: English
Volume: 214
Issue: 4520
Pages: 562-564

Researcher Affiliations

Studdert, M J
    Simpson, T
      Roizman, B

        MeSH Terms

        • Abortion, Veterinary / microbiology
        • Animals
        • DNA Restriction Enzymes
        • DNA, Viral / genetics
        • Female
        • Fetus / microbiology
        • Herpesviridae / genetics
        • Herpesvirus 1, Equid / genetics
        • Horse Diseases / microbiology
        • Horses
        • Pregnancy

        Grant Funding

        • CA 08494 / NCI NIH HHS
        • CA 09241 / NCI NIH HHS
        • CA 19264 / NCI NIH HHS

        Citations

        This article has been cited 30 times.
        1. El Brini Z, Fassi Fihri O, Paillot R, Lotfi C, Amraoui F, El Ouadi H, Dehhaoui M, Colitti B, Alyakine H, Piro M. Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations. Animals (Basel) 2021 Sep 29;11(10).
          doi: 10.3390/ani11102851pubmed: 34679874google scholar: lookup
        2. Pavulraj S, Eschke K, Theisen J, Westhoff S, Reimers G, Andreotti S, Osterrieder N, Azab W. Equine Herpesvirus Type 4 (EHV-4) Outbreak in Germany: Virological, Serological, and Molecular Investigations. Pathogens 2021 Jun 25;10(7).
          doi: 10.3390/pathogens10070810pubmed: 34202127google scholar: lookup
        3. Gupta AK, Kaur D, Rattan B, Yadav MP. Molecular variability in different Indian isolates of equine herpesvirus-1. Vet Res Commun 2005 Nov;29(8):721-34.
          doi: 10.1007/s11259-005-3380-zpubmed: 16369886google scholar: lookup
        4. Molinková D, Celer V Jr, Jahn P. Isolation and partial characterization of equine herpesvirus type 1 in Czechia. Folia Microbiol (Praha) 2004;49(5):605-11.
          doi: 10.1007/BF02931542pubmed: 15702554google scholar: lookup
        5. Kirisawa R, Ohmori H, Iwai H, Kawakami Y. The genomic diversity among equine herpesvirus-1 strains isolated in Japan. Arch Virol 1993;129(1-4):11-22.
          doi: 10.1007/BF01316881pubmed: 8385910google scholar: lookup
        6. Sinclair R, Binns MM, Chirnside ED, Mumford JA. Detection of antibodies against equine herpesvirus types 1 and 4 by using recombinant protein derived from an immunodominant region of glycoprotein B. J Clin Microbiol 1993 Feb;31(2):265-71.
          doi: 10.1128/jcm.31.2.265-271.1993pubmed: 8381809google scholar: lookup
        7. Nagesha HS, Crabb BS, Studdert MJ. Analysis of the nucleotide sequence of five genes at the left end of the unique short region of the equine herpesvirus 4 genome. Arch Virol 1993;128(1-2):143-54.
          doi: 10.1007/BF01309795pubmed: 8380320google scholar: lookup
        8. Crabb BS, Studdert MJ. Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host. J Virol 1993 Oct;67(10):6332-8.
          doi: 10.1128/JVI.67.10.6332-6338.1993pubmed: 7690425google scholar: lookup
        9. van de Moer A, Rice M, Wilks CR. A type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis. Arch Virol 1993;132(1-2):133-44.
          doi: 10.1007/BF01309848pubmed: 7688948google scholar: lookup
        10. Kamada M, Studdert MJ. Analysis of small and large plaque variants of equine herpesvirus type 3 by restriction endonucleases. Brief report. Arch Virol 1983;77(2-4):259-64.
          doi: 10.1007/BF01309273pubmed: 6314939google scholar: lookup
        11. Studdert MJ. Restriction endonuclease DNA fingerprinting of respiratory, foetal and perinatal foal isolates of equine herpesvirus type 1. Arch Virol 1983;77(2-4):249-58.
          doi: 10.1007/BF01309272pubmed: 6314938google scholar: lookup
        12. Paul PS, Mengeling WL, Pirtle EC. Differentiation of pseudorabies (Aujeszky's disease) virus strains by restriction endonuclease analysis. Arch Virol 1982;73(2):193-8.
          doi: 10.1007/BF01314727pubmed: 6293411google scholar: lookup
        13. Allen GP, Turtinen LW. Assessment of the base sequence homology between the two subtypes of equine herpesvirus 1. J Virol 1982 Oct;44(1):249-55.
          doi: 10.1128/JVI.44.1.249-255.1982pubmed: 6292488google scholar: lookup
        14. Desrosiers RC, Falk LA. Herpesvirus saimiri strain variability. J Virol 1982 Jul;43(1):352-6.
          doi: 10.1128/JVI.43.1.352-356.1982pubmed: 6287011google scholar: lookup
        15. Medveczky P, Szomolanyi E, Desrosiers RC, Mulder C. Classification of herpesvirus saimiri into three groups based on extreme variation in a DNA region required for oncogenicity. J Virol 1984 Dec;52(3):938-44.
          doi: 10.1128/JVI.52.3.938-944.1984pubmed: 6092721google scholar: lookup
        16. Studdert MJ, Fitzpatrick DR, Browning GF, Cullinane AA, Whalley JM. Equine herpesvirus genomes: heterogeneity of naturally occurring type 4 isolates and of a type 1 isolate after heterologous cell passage. Arch Virol 1986;91(3-4):375-81.
          doi: 10.1007/BF01314296pubmed: 3022687google scholar: lookup
        17. Chowdhury SI, Kubin G, Ludwig H. Equine herpesvirus type 1 (EHV-1) induced abortions and paralysis in a Lipizzaner stud: a contribution to the classification of equine herpesviruses. Arch Virol 1986;90(3-4):273-88.
          doi: 10.1007/BF01317376pubmed: 3015084google scholar: lookup
        18. Yeargan MR, Allen GP, Bryans JT. Rapid subtyping of equine herpesvirus 1 with monoclonal antibodies. J Clin Microbiol 1985 May;21(5):694-7.
          doi: 10.1128/jcm.21.5.694-697.1985pubmed: 2987299google scholar: lookup
        19. Thiermann AB, Handsaker AL, Moseley SL, Kingscote B. New method for classification of leptospiral isolates belonging to serogroup pomona by restriction endonuclease analysis: serovar kennewicki. J Clin Microbiol 1985 Apr;21(4):585-7.
          doi: 10.1128/jcm.21.4.585-587.1985pubmed: 2985648google scholar: lookup
        20. Browning GF, Ficorilli N, Studdert MJ. Asinine herpesvirus genomes: comparison with those of the equine herpesviruses. Arch Virol 1988;101(3-4):183-90.
          doi: 10.1007/BF01310999pubmed: 2845891google scholar: lookup
        21. Shih LM, Zee YC, Castro AE. Comparison of genomes of malignant catarrhal fever-associated herpesviruses by restriction endonuclease analysis. Arch Virol 1989;109(1-2):145-51.
          doi: 10.1007/BF01310526pubmed: 2558629google scholar: lookup
        22. Whetstone CA, Miller JM, Bortner DM, Van der Maaten MJ. Changes in the bovine herpesvirus 1 genome during acute infection, after reactivation from latency, and after superinfection in the host animal. Arch Virol 1989;106(3-4):261-79.
          doi: 10.1007/BF01313957pubmed: 2549919google scholar: lookup
        23. Bridges CG, Edington N. Innate immunity during Equid herpesvirus 1 (EHV-1) infection. Clin Exp Immunol 1986 Jul;65(1):172-81.
          pubmed: 2431815
        24. Staczek J. Animal cytomegaloviruses. Microbiol Rev 1990 Sep;54(3):247-65.
          doi: 10.1128/mr.54.3.247-265.1990pubmed: 2170830google scholar: lookup
        25. Cornick J, Martens J, Martens R, Crandell R, McConnell S, Kit S. Safety and efficacy of a thymidine kinase negative equine herpesvirus-1 vaccine in young horses. Can J Vet Res 1990 Apr;54(2):260-6.
          pubmed: 2162730
        26. Chowdhury SI, Buhk HJ, Ludwig H, Hammerschmidt W. Genomic termini of equine herpesvirus 1. J Virol 1990 Feb;64(2):873-80.
          doi: 10.1128/JVI.64.2.873-880.1990pubmed: 2153249google scholar: lookup
        27. Roizmann B, Desrosiers RC, Fleckenstein B, Lopez C, Minson AC, Studdert MJ. The family Herpesviridae: an update. The Herpesvirus Study Group of the International Committee on Taxonomy of Viruses. Arch Virol 1992;123(3-4):425-49.
          doi: 10.1007/BF01317276pubmed: 1562239google scholar: lookup
        28. Nagesha HS, McNeil JR, Ficorilli N, Studdert MJ. Cloning and restriction endonuclease mapping of the genome of an equine herpesvirus 4 (equine rhinopneumonitis virus), strain 405/76. Arch Virol 1992;124(3-4):379-87.
          doi: 10.1007/BF01309818pubmed: 1318713google scholar: lookup
        29. Normand C, Thieulent CJ, Fortier C, Sutton G, Senamaud-Beaufort C, Jourdren L, Blugeon C, Vidalain PO, Pronost S, Hue ES. A Screening Study Identified Decitabine as an Inhibitor of Equid Herpesvirus 4 That Enhances the Innate Antiviral Response. Viruses 2024 May 8;16(5).
          doi: 10.3390/v16050746pubmed: 38793627google scholar: lookup
        30. Rimstad E, Hyllseth B. Equine herpesviruses 1 and 4: amplification and differentiation by polymerase chain reaction. Acta Vet Scand 1994;35(3):303-6.
          doi: 10.1186/BF03548336pubmed: 7847200google scholar: lookup
        Subscribe to our newsletter
        Join 99,359 horse owners like you who receive our email updates on horse health and nutrition.