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Home / Equine Research Bank / Can J Vet Res / [Direct approach for demonstrating free radical phenomena du...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire1994; 58(4); 309-312;

[Direct approach for demonstrating free radical phenomena during equine postanesthetic myopathy: preliminary study].

Abstract: This preliminary study demonstrated the existence of a free radical generation during an experimental postischemic muscular reperfusion in a halothane anesthetized horse. The authors used alpha-phényl-N-tert-butylnitrone as a spin trap agent and the electronic paramagnetic resonance method to observe in vivo a free radical generation. This preliminary study demonstrated the existence of a free radical generation during an experimental postischemic muscular reperfusion in a halothane anesthetized horse. The authors used alpha-phényl-N-tert-butylnitrone as a spin trap agent and the electronic paramagnetic resonance method to observe in vivo a free radical generation.
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Publication Date: 1994-10-01 PubMed ID: 7889465PubMed Central: PMC1263720
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  • English Abstract
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores the production of free radicals during muscle reperfusion after ischemia in horses under halothane anesthesia, evidenced through the use of a specific agent and a detection method.

Research Objective and Methodology

  • The primary aim of this study was to discern whether free radicals – molecules or atoms with an unpaired electron that can be harmful – are produced during the recovery phase (reperfusion) following a lack of blood supply (ischemia) to the muscles of anaesthetized horses.
  • The authors implemented an experimental approach to stimulate ischemia and subsequent reperfusion in the muscles of horses anesthetized with halothane, a potent inhalation anesthetic particularly used in veterinary medicine.
  • To detect the generation of free radicals during this process, the researchers utilized a compound known as alpha-phényl-N-tert-butylnitrone. This substance, commonly known as a spin trap agent, has the ability to trap and stabilize the free radicals formed, thereby allowing for their detection and study.
  • The specific technique used to visualize the trapped free radicals was electronic paramagnetic resonance (EPR), a complex method that enables the identification of substances with unpaired electrons, such as free radicals, in a living organism.

Findings and Implications

  • The results from this preliminary investigation confirmed the production of free radicals during the reperfusion stage post-ischemia in the tested halothane anesthetized horse.
  • The findings of this study are significant because they demonstrate a phenomenon often associated with cell and tissue damage. Increased production of free radicals, or oxidative stress, during reperfusion has been implicated in numerous pathologies, including ischemia-reperfusion injury that often leads to significant tissue damage and can cause a variety of problems such as heart attacks or strokes in humans.
  • Therefore, understanding this process in the context of equine health could open avenues for creating effective strategies to mitigate the harmful effects caused by the overproduction of free radicals during muscle reperfusion after periods of ischemia.
  • However, it’s necessary to note this is a preliminary study. Further research with a larger and diverse sample size is required to validate these results and better comprehend the mechanisms underlying this phenomenon.

Cite This Article

APA
Serteyn D, Pincemail J, Mottart E, Caudron I, Deby C, Deby-Dupont G, Philippart C, Lamy M. (1994). [Direct approach for demonstrating free radical phenomena during equine postanesthetic myopathy: preliminary study]. Can J Vet Res, 58(4), 309-312.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: fre
Volume: 58
Issue: 4
Pages: 309-312

Researcher Affiliations

Serteyn, D
  • Service de chirurgie des grands animaux et d'anesthésiologie, Faculté de médecine vétérinaire, Université de Liège, Belgique.
Pincemail, J
    Mottart, E
      Caudron, I
        Deby, C
          Deby-Dupont, G
            Philippart, C
              Lamy, M

                MeSH Terms

                • Anesthesia, Inhalation / adverse effects
                • Anesthesia, Inhalation / veterinary
                • Animals
                • Cyclic N-Oxides
                • Female
                • Free Radicals / metabolism
                • Halothane / adverse effects
                • Horse Diseases / etiology
                • Horse Diseases / metabolism
                • Horses
                • Muscular Diseases / metabolism
                • Muscular Diseases / veterinary
                • Nitrogen Oxides
                • Reperfusion Injury / metabolism
                • Reperfusion Injury / veterinary
                • Spin Labels

                References

                This article includes 16 references
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                Citations

                This article has been cited 2 times.
                1. Benbarek H, Ayad A, Deby-Dupont G, Boukraa L, Serteyn D. Modulatory effects of non-steroidal anti-inflammatory drugs on the luminol and lucigenin amplified chemiluminescence of equine neutrophils. Vet Res Commun 2012 Mar;36(1):29-33.
                  doi: 10.1007/s11259-011-9507-5pubmed: 22012073google scholar: lookup
                2. Edner AH, Essén-Gustavsson B, Nyman GC. Metabolism during anaesthesia and recovery in colic and healthy horses: a microdialysis study. Acta Vet Scand 2009 Mar 10;51(1):10.
                  doi: 10.1186/1751-0147-51-10pubmed: 19284560google scholar: lookup
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