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Home / Equine Research Bank / Open Life Sci / Discovery of exercise-related genes and pathway analysis bas...
Open life sciences2022; 17(1); 1269-1281; doi: 10.1515/biol-2022-0487

Discovery of exercise-related genes and pathway analysis based on comparative genomes of Mongolian originated Abaga and Wushen horse.

Abstract: The Mongolian horses have excellent endurance and stress resistance to adapt to the cold and harsh plateau conditions. Intraspecific genetic diversity is mainly embodied in various genetic advantages of different branches of the Mongolian horse. Since people pay progressive attention to the athletic performance of horse, we expect to guide the exercise-oriented breeding of horses through genomics research. We obtained the clean data of 630,535,376,400 bp through the entire genome second-generation sequencing for the whole blood of four Abaga horses and ten Wushen horses. Based on the data analysis of single nucleotide polymorphism, we severally detected that 479 and 943 positively selected genes, particularly exercise related, were mainly enriched on equine chromosome 4 in Abaga horses and Wushen horses, which implied that chromosome 4 may be associated with the evolution of the Mongolian horse and athletic performance. Four hundred and forty genes of positive selection were enriched in 12 exercise-related pathways and narrowed in 21 exercise-related genes in Abaga horse, which were distinguished from Wushen horse. So, we speculated that the Abaga horse may have oriented genes for the motorial mechanism and 21 exercise-related genes also provided a molecular genetic basis for exercise-directed breeding of the Mongolian horse.
© 2022 Jing Pan et al., published by De Gruyter.
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Publication Date: 2022-09-26 PubMed ID: 36249530PubMed Central: PMC9518662DOI: 10.1515/biol-2022-0487Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the genetic differences between two branches of the Mongolian horse, focusing particularly on their exercise-related genes. The study, based on full genome sequencing, found that the number and distribution of these genes varied between the Abaga and Wushen horses, providing insight that could guide breeding for athletic performance.

Research Goal and Methodology

  • The research aimed to understand genetic differences related to exercise and athletic performance in two branches of the Mongolian horse – the Abaga and Wushen breed.
  • Genomic data of these horses was collected through full genome sequencing, with total clean data of 630,535,376,400 bp from the blood samples of four Abaga horses and ten Wushen horses.
  • The data was analyzed focusing on single nucleotide polymorphism, a type of genetic variation, to identify positively selected genes, which have been favored by natural selection.

Key Findings

  • From the collected data, 479 and 943 positively selected genes were found in Abaga and Wushen horses, respectively.
  • These selected genes were predominantly enriched on equine chromosome 4. This finding suggests that chromosome 4 may be responsible for evolution and athletic performance in these Mongolian horses.
  • A smaller subset of 440 positively selected genes were identified as part of 12 exercise-related pathways and were specific to the Abaga breed. This subset was narrowed down further to 21 exercise-related genes.

Implications of the Research

  • These findings indicate that Abaga and Wushen horses have genetically different exercise capabilities, and the Abaga breed may have a genetic predisposition for certain motor functions.
  • The identified 21 exercise-related genes could provide a basis for breeding Mongolian horses for specific athletic performances.
  • Such bench-marking of exercise-related genes could potentially assist in enhancing athletic performance in other equine breeds as well.

Cite This Article

APA
Pan J, Purev C, Zhao H, Zhang Z, Wang F, Wendoule N, Qi G, Liu Y, Zhou H. (2022). Discovery of exercise-related genes and pathway analysis based on comparative genomes of Mongolian originated Abaga and Wushen horse. Open Life Sci, 17(1), 1269-1281. https://doi.org/10.1515/biol-2022-0487

Publication

Open life sciences
ISSN: 2391-5412
NlmUniqueID: 101669614
Country: Poland
Language: English
Volume: 17
Issue: 1
Pages: 1269-1281

Researcher Affiliations

Pan, Jing
  • Faculty of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region, People's Republic of China.
  • Department of Reproductive Medicine, Inner Mongolia Maternal and Child Health Care Hospitaly, Hohhot, Inner Mongolia Autonomous Region, People's Republic of China.
Purev, Chimge
  • Mongolia-China Joint Laboratory of Applied Molecular Biology, "Administration of the Science Park" CSTI, Ulaanbaatar, Mongolia.
Zhao, Hongwei
  • Beijing 8omics Gene Technology Co. Ltd, Beijing, People's Republic of China.
Zhang, Zhipeng
  • Faculty of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region, People's Republic of China.
Wang, Feng
  • Faculty of Life Sciences, Nankai University, Tianjin, People's Republic of China.
Wendoule, Nashun
  • Animal Husbandry Workstation of Ewenki Autonomous County, Hulun Buir, Inner Mongolia Autonomous Region, People's Republic of China.
Qi, Guichun
  • Bayanta Village of Animal Husbandry and Veterinary Station of Ewenki Autonomous County, Hulun Buir, Inner Mongolia Autonomous Region, People's Republic of China.
Liu, Yongbin
  • Sheep Collaboration and Innovation Center, Inner Mongolia Universityy, Hohhot, Inner Mongolia Autonomous Region, People's Republic of China.
Zhou, Huanmin
  • Faculty of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region, People's Republic of China.
  • Sheep Collaboration and Innovation Center, Inner Mongolia Universityy, Hohhot, Inner Mongolia Autonomous Region, People's Republic of China.

Conflict of Interest Statement

Conflict of interest: Authors state no conflict of interest.

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