Discovery proteomics for the detection of putative markers for eradication of infection in an experimental model of equine septic arthritis using LC-MS/MS.
Abstract: Septic arthritis (SA) is a life-threatening condition in horses, and identifying eradication of infection in equine SA is challenging. This study explored the discovery of putative biomarkers for the eradication of joint infection in horses. We performed proteomics analysis of synovial fluid (SF) and plasma from horses with experimental SA, non-septic lipopolysaccharide-induced arthritis, and controls. The point of eradication of infection in horses with SA was determined previously. We compared spectral intensities between groups as well as before and after the eradication of infection. Twenty-six differentially abundant proteins were identified, which were upregulated in SF of horses with SA compared to the other groups, as well as compared to the same horses post-eradication of infection. In plasma, we did not identify differentially abundant proteins. Differentially abundant proteins in SF were of cellular origin and their biological functions included ubiquitination, signal transduction, apoptosis etc. The difference in their relative abundance between experimental groups was ≥10-fold compared to the abundance expected based on the difference in cell count alone (2-fold). Since most of cells in joints with bacterial infection are neutrophils, we suggest that the variable abundance of neutrophil- and cell-associated proteins represent potential biomarkers of eradication of infection in equine SA. SIGNIFICANCE: Septic arthritis is an important condition in horses, which can be life-threatening. At present, identifying eradication of infection in cases of equine septic arthritis is challenging. In this study, we performed a global proteomics analysis of synovial fluid and plasma in horses with experimental septic arthritis and identified 26 differentially abundant proteins compared to non-septic arthritis and post eradication of infection. The results of this study provide the basis for further characterization of the differentially abundant proteins and identification of clinically relevant biomarkers of septic arthritis in horses.
Copyright © 2022 Elsevier B.V. All rights reserved.
Publication Date: 2022-03-26 PubMed ID: 35346876DOI: 10.1016/j.jprot.2022.104571Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explores the potential biomarkers for the eradication of joint infection in horses. The analysis revealed 26 proteins that were more abundant in the synovial fluid of horses with septic arthritis than in other groups or in the same horses post-infection. This opens a path for further exploration and identification of clinically relevant biomarkers for septic arthritis in horses.
Objective and Methodology
- The goal of this study was to identify possible biomarkers that could signal the eradication of joint infection in horses suffering from septic arthritis. This condition is serious and potentially fatal, and current methods for identifying when the infection has been eradicated are not optimal.
- To discover these possible markers, the researchers performed a proteomics analysis, a large-scale study of proteins, on the synovial fluid and plasma of horses with septic arthritis, horses with non-septic lipopolysaccharide-induced arthritis, and control horses.
- The point at which the infection had been eradicated in the septic horses was determined beforehand.
Findings
- After comparing the spectral intensities between the groups both before and after the eradication of infection, the researchers found 26 proteins that were upregulated (i.e., more abundant) in the synovial fluid of the horses with septic arthritis.
- Interestingly, these 26 proteins were more than ten times as abundant as would be expected based on the difference in cell count alone, suggesting that these proteins could be potential markers for infection eradication.
- Most of these proteins were of cellular origin, and their primary biological functions were in areas like signal transduction, apoptosis – the process of programmed cell death, and ubiquitination – a process where proteins are marked for degradation.
- In the plasma of the horses, no differentially abundant proteins were found.
Significance
- Septic arthritis is a serious and potentially fatal condition in horses, and better ways of determining when an infection has been eradicated are needed. This study’s identification of 26 potentially significant proteins opens a path for future research and the eventual identification of clinically useful biomarkers for this condition.
- The study suggests that the variable abundance of neutrophil- and cell-associated proteins in the synovial fluid could act as potential biomarkers for infection eradication in equine septic arthritis.
- This discovery represents a significant contribution to the ongoing struggle against septic arthritis in horses and could form the basis of further research.
Cite This Article
APA
Koziy RV, Bracamonte JL, Yoshimura S, Chumala P, Simko E, Katselis GS.
(2022).
Discovery proteomics for the detection of putative markers for eradication of infection in an experimental model of equine septic arthritis using LC-MS/MS.
J Proteomics, 261, 104571.
https://doi.org/10.1016/j.jprot.2022.104571 Publication
Researcher Affiliations
- Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada. Electronic address: roman.koziy@usask.ca.
- Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
- Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
- Department of Medicine, Division of Canadian Centre for Health and Safety in Agriculture, College of Medicine, University of Saskatchewan, 104 Clinic Place, Saskatoon, Saskatchewan S7N 2Z4, Canada.
- Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
- Department of Medicine, Division of Canadian Centre for Health and Safety in Agriculture, College of Medicine, University of Saskatchewan, 104 Clinic Place, Saskatoon, Saskatchewan S7N 2Z4, Canada. Electronic address: george.katselis@usask.ca.
MeSH Terms
- Animals
- Arthritis, Infectious / diagnosis
- Arthritis, Infectious / metabolism
- Arthritis, Infectious / veterinary
- Biomarkers / metabolism
- Chromatography, Liquid
- Horse Diseases / diagnosis
- Horse Diseases / metabolism
- Horses
- Models, Theoretical
- Proteomics
- Synovial Fluid / metabolism
- Tandem Mass Spectrometry
Citations
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