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The Journal of veterinary medical science2015; 78(2); 287-291; doi: 10.1292/jvms.15-0406

Distribution of CD163-positive cell and MHC class II-positive cell in the normal equine uveal tract.

Abstract: Antigen-presenting cells (APCs) in the uveal tract participate in ocular immunity including immune homeostasis and the pathogenesis of uveitis. In horses, although uveitis is the most common ocular disorder, little is known about ocular immunity, such as the distribution of APCs. In this study, we investigated the distribution of CD163-positive and MHC II-positive cells in the normal equine uveal tract using an immunofluorescence technique. Eleven eyes from 10 Thoroughbred horses aged 1 to 24 years old were used. Indirect immunofluorescence was performed using the primary antibodies CD163, MHC class II (MHC II) and CD20. To demonstrate the site of their greatest distribution, positive cells were manually counted in 3 different parts of the uveal tract (ciliary body, iris and choroid), and their average number was assessed by statistical analysis. The distribution of pleomorphic CD163- and MHC II-expressed cells was detected throughout the equine uveal tract, but no CD20-expressed cells were detected. The statistical analysis demonstrated the distribution of CD163- and MHC II-positive cells focusing on the ciliary body. These results demonstrated that the ciliary body is the largest site of their distribution in the normal equine uveal tract, and the ciliary body is considered to play important roles in uveal and/or ocular immune homeostasis. The data provided in this study will help further understanding of equine ocular immunity in the normal state and might be beneficial for understanding of mechanisms of ocular disorders, such as equine uveitis.
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Publication Date: 2015-11-03 PubMed ID: 26537548PubMed Central: PMC4785119DOI: 10.1292/jvms.15-0406Google Scholar: Lookup
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  • Journal Article

Summary

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The research article focuses on a study that investigates the distribution of antigen-presenting cells (APCs) CD163-positive and MHC II-positive in the normal equine uveal tract. The results show their highest concentration in the ciliary body, contributing to understanding equine ocular immunity and ocular disorders such as uveitis.

Research Objective and Approach

  • The primary goal of the study was to investigate the distribution of CD163-positive and MHC II-positive cells in the normal uveal tract of horses. This was done with the aid of an immunofluorescence technique.
  • The research is important as the highest common ocular disorder in horses is uveitis, yet there’s minimal knowledge regarding ocular immunity, including the distribution of APCs. The findings help in understanding ocular immunity and uveitis.

Methodology and Sample

  • The study examined eleven eyes from ten Thoroughbred horses aged between 1 and 24 years old.
  • To identify the primary site of distribution, the researchers manually counted positive cells in three different parts of the uveal tract: the ciliary body, iris, and choroid. They then made statistical analyses of the average cell number.

Findings

  • The researchers found evidence of pleomorphic CD163- and MHC II-expressed cells throughout the equine uveal tract. No CD20-expressed cells were, however, detected.
  • The statistical analysis showed that the distribution of CD163- and MHC II-positive cells was concentrated on the ciliary body, indicating it as the largest site of their distribution in the normal equine uveal tract.
  • The data, therefore, suggested that the ciliary body plays significant roles in uveal and/or ocular immune homeostasis.

Implications

  • The results of this research assist in further understanding equine ocular immunity under normal conditions. They could also be beneficial in understanding the mechanisms of ocular disorders like equine uveitis.
  • Such knowledge about APCs in horses’ eyes can potentially help veterinarians better identify and treat ocular disorders in horses.

Cite This Article

APA
Sano Y, Matsuda K, Okamoto M, Takehana K, Hirayama K, Taniyama H. (2015). Distribution of CD163-positive cell and MHC class II-positive cell in the normal equine uveal tract. J Vet Med Sci, 78(2), 287-291. https://doi.org/10.1292/jvms.15-0406

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 78
Issue: 2
Pages: 287-291

Researcher Affiliations

Sano, Yuto
  • Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Matsuda, Kazuya
    Okamoto, Minoru
      Takehana, Kazushige
        Hirayama, Kazuko
          Taniyama, Hiroyuki

            MeSH Terms

            • Animals
            • Antigens, CD / analysis
            • Antigens, CD20 / analysis
            • Antigens, Differentiation, Myelomonocytic / analysis
            • Cell Count
            • Ciliary Body / cytology
            • Female
            • Fluorescent Antibody Technique, Indirect / veterinary
            • Genes, MHC Class II
            • Horses
            • Macrophages / cytology
            • Macrophages / immunology
            • Male
            • Receptors, Cell Surface / analysis
            • Reference Values
            • Uvea / cytology
            • Uveitis / immunology
            • Uveitis / veterinary

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            Citations

            This article has been cited 1 times.
            1. Kang H, Bienzle D, Lee GKC, Piché É, Viel L, Odemuyiwa SO, Beeler-Marfisi J. Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma. Vet Pathol 2022 Jan;59(1):91-99.
              doi: 10.1177/03009858211042588pubmed: 34521286google scholar: lookup
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