FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Vet Med Sci / Distribution of CD163-positive cell and MHC class II-positiv...
The Journal of veterinary medical science2015; 78(2); 287-291; doi: 10.1292/jvms.15-0406

Distribution of CD163-positive cell and MHC class II-positive cell in the normal equine uveal tract.

Abstract: Antigen-presenting cells (APCs) in the uveal tract participate in ocular immunity including immune homeostasis and the pathogenesis of uveitis. In horses, although uveitis is the most common ocular disorder, little is known about ocular immunity, such as the distribution of APCs. In this study, we investigated the distribution of CD163-positive and MHC II-positive cells in the normal equine uveal tract using an immunofluorescence technique. Eleven eyes from 10 Thoroughbred horses aged 1 to 24 years old were used. Indirect immunofluorescence was performed using the primary antibodies CD163, MHC class II (MHC II) and CD20. To demonstrate the site of their greatest distribution, positive cells were manually counted in 3 different parts of the uveal tract (ciliary body, iris and choroid), and their average number was assessed by statistical analysis. The distribution of pleomorphic CD163- and MHC II-expressed cells was detected throughout the equine uveal tract, but no CD20-expressed cells were detected. The statistical analysis demonstrated the distribution of CD163- and MHC II-positive cells focusing on the ciliary body. These results demonstrated that the ciliary body is the largest site of their distribution in the normal equine uveal tract, and the ciliary body is considered to play important roles in uveal and/or ocular immune homeostasis. The data provided in this study will help further understanding of equine ocular immunity in the normal state and might be beneficial for understanding of mechanisms of ocular disorders, such as equine uveitis.
Get Full Text
Publication Date: 2015-11-03 PubMed ID: 26537548PubMed Central: PMC4785119DOI: 10.1292/jvms.15-0406Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on a study that investigates the distribution of antigen-presenting cells (APCs) CD163-positive and MHC II-positive in the normal equine uveal tract. The results show their highest concentration in the ciliary body, contributing to understanding equine ocular immunity and ocular disorders such as uveitis.

Research Objective and Approach

  • The primary goal of the study was to investigate the distribution of CD163-positive and MHC II-positive cells in the normal uveal tract of horses. This was done with the aid of an immunofluorescence technique.
  • The research is important as the highest common ocular disorder in horses is uveitis, yet there’s minimal knowledge regarding ocular immunity, including the distribution of APCs. The findings help in understanding ocular immunity and uveitis.

Methodology and Sample

  • The study examined eleven eyes from ten Thoroughbred horses aged between 1 and 24 years old.
  • To identify the primary site of distribution, the researchers manually counted positive cells in three different parts of the uveal tract: the ciliary body, iris, and choroid. They then made statistical analyses of the average cell number.

Findings

  • The researchers found evidence of pleomorphic CD163- and MHC II-expressed cells throughout the equine uveal tract. No CD20-expressed cells were, however, detected.
  • The statistical analysis showed that the distribution of CD163- and MHC II-positive cells was concentrated on the ciliary body, indicating it as the largest site of their distribution in the normal equine uveal tract.
  • The data, therefore, suggested that the ciliary body plays significant roles in uveal and/or ocular immune homeostasis.

Implications

  • The results of this research assist in further understanding equine ocular immunity under normal conditions. They could also be beneficial in understanding the mechanisms of ocular disorders like equine uveitis.
  • Such knowledge about APCs in horses’ eyes can potentially help veterinarians better identify and treat ocular disorders in horses.

Cite This Article

APA
Sano Y, Matsuda K, Okamoto M, Takehana K, Hirayama K, Taniyama H. (2015). Distribution of CD163-positive cell and MHC class II-positive cell in the normal equine uveal tract. J Vet Med Sci, 78(2), 287-291. https://doi.org/10.1292/jvms.15-0406

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 78
Issue: 2
Pages: 287-291

Researcher Affiliations

Sano, Yuto
  • Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Matsuda, Kazuya
    Okamoto, Minoru
      Takehana, Kazushige
        Hirayama, Kazuko
          Taniyama, Hiroyuki

            MeSH Terms

            • Animals
            • Antigens, CD / analysis
            • Antigens, CD20 / analysis
            • Antigens, Differentiation, Myelomonocytic / analysis
            • Cell Count
            • Ciliary Body / cytology
            • Female
            • Fluorescent Antibody Technique, Indirect / veterinary
            • Genes, MHC Class II
            • Horses
            • Macrophages / cytology
            • Macrophages / immunology
            • Male
            • Receptors, Cell Surface / analysis
            • Reference Values
            • Uvea / cytology
            • Uveitis / immunology
            • Uveitis / veterinary

            References

            This article includes 20 references
            1. Bakker M, Kijlstra A. The expression of HLA-antigens in the human anterior uvea.. Curr Eye Res 1985 May;4(5):599-604.
              doi: 10.3109/02713688508999991pubmed: 3893899google scholar: lookup
            2. Barnett KC. Iris and ciliary body. 2004. pp. 183–199. In: Equine Ophthalmology, 2nd ed..
            3. Davidson MG. Equine ophthalmology. 1991. pp. 576–610. In: Veterinary Ophthalmology, 2nd ed..
            4. Davies LC, Jenkins SJ, Allen JE, Taylor PR. Tissue-resident macrophages.. Nat Immunol 2013 Oct;14(10):986-95.
              doi: 10.1038/ni.2705pmc: PMC4045180pubmed: 24048120google scholar: lookup
            5. Fabriek BO, Dijkstra CD, van den Berg TK. The macrophage scavenger receptor CD163.. Immunobiology 2005;210(2-4):153-60.
              doi: 10.1016/j.imbio.2005.05.010pubmed: 16164022google scholar: lookup
            6. Fabriek BO, van Bruggen R, Deng DM, Ligtenberg AJ, Nazmi K, Schornagel K, Vloet RP, Dijkstra CD, van den Berg TK. The macrophage scavenger receptor CD163 functions as an innate immune sensor for bacteria.. Blood 2009 Jan 22;113(4):887-92.
              doi: 10.1182/blood-2008-07-167064pubmed: 18849484google scholar: lookup
            7. Hines MT. Immunologically mediated ocular disease in the horse.. Vet Clin North Am Large Anim Pract 1984 Nov;6(3):501-12.
              pubmed: 6393544doi: 10.1016/s0196-9846(17)30006-xgoogle scholar: lookup
            8. Knisely TL, Anderson TM, Sherwood ME, Flotte TJ, Albert DM, Granstein RD. Morphologic and ultrastructural examination of I-A+ cells in the murine iris.. Invest Ophthalmol Vis Sci 1991 Jul;32(8):2423-31.
              pubmed: 2071354
            9. Matthews AG. Uveitis in the horse: a review of the aetiological and immunopathological aspects of the disease. Equine Vet. J. 1983 15S2: 61–64.
              doi: 10.1111/j.2042-3306.1983.tb04561.xgoogle scholar: lookup
            10. McMenamin PG. Dendritic cells and macrophages in the uveal tract of the normal mouse eye.. Br J Ophthalmol 1999 May;83(5):598-604.
              doi: 10.1136/bjo.83.5.598pmc: PMC1723050pubmed: 10216062google scholar: lookup
            11. McMenamin PG. The distribution of immune cells in the uveal tract of the normal eye.. Eye (Lond) 1997;11 ( Pt 2):183-93.
              doi: 10.1038/eye.1997.49pubmed: 9349410google scholar: lookup
            12. McMenamin PG, Crewe J, Morrison S, Holt PG. Immunomorphologic studies of macrophages and MHC class II-positive dendritic cells in the iris and ciliary body of the rat, mouse, and human eye.. Invest Ophthalmol Vis Sci 1994 Jul;35(8):3234-50.
              pubmed: 8045716
            13. McMenamin PG, Holthouse I, Holt PG. Class II major histocompatibility complex (Ia) antigen-bearing dendritic cells within the iris and ciliary body of the rat eye: distribution, phenotype and relation to retinal microglia.. Immunology 1992 Nov;77(3):385-93.
              pmc: PMC1421726pubmed: 1478684
            14. Niederkorn JY. Immune privilege in the anterior chamber of the eye.. Crit Rev Immunol 2002;22(1):13-46.
              doi: 10.1615/CritRevImmunol.v22.i1.20pubmed: 12186187google scholar: lookup
            15. Romeike A, Brügmann M, Drommer W. Immunohistochemical studies in equine recurrent uveitis (ERU).. Vet Pathol 1998 Nov;35(6):515-26.
              doi: 10.1177/030098589803500606pubmed: 9823593google scholar: lookup
            16. Steinman RM. The dendritic cell system and its role in immunogenicity.. Annu Rev Immunol 1991;9:271-96.
              doi: 10.1146/annurev.iy.09.040191.001415pubmed: 1910679google scholar: lookup
            17. Stein-Streilein J. Immune regulation and the eye.. Trends Immunol 2008 Nov;29(11):548-54.
              doi: 10.1016/j.it.2008.08.002pubmed: 18838303google scholar: lookup
            18. Snyder PW. Disease of immunology. 2012. pp. 242–288. In: Pathologic Basis of Veterinary Disease, 5th ed..
            19. Wang HM, Kaplan HJ, Chan WC, Johnson M. The distribution and ontogeny of MHC antigens in murine ocular tissue.. Invest Ophthalmol Vis Sci 1987 Aug;28(8):1383-9.
              pubmed: 3301731
            20. Yamate J, Yoshida H, Tsukamoto Y, Ide M, Kuwamura M, Ohashi F, Miyamoto T, Kotani T, Sakuma S, Takeya M. Distribution of cells immunopositive for AM-3K, a novel monoclonal antibody recognizing human macrophages, in normal and diseased tissues of dogs, cats, horses, cattle, pigs, and rabbits.. Vet Pathol 2000 Mar;37(2):168-76.
              doi: 10.1354/vp.37-2-168pubmed: 10714646google scholar: lookup

            Citations

            This article has been cited 1 times.
            1. Kang H, Bienzle D, Lee GKC, Piché É, Viel L, Odemuyiwa SO, Beeler-Marfisi J. Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma. Vet Pathol 2022 Jan;59(1):91-99.
              doi: 10.1177/03009858211042588pubmed: 34521286google scholar: lookup
            Subscribe to our newsletter
            Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.