Distribution of voriconazole in seven body fluids of adult horses after repeated oral dosing.

The research article investigates the distribution and safety of voriconazole, a medication, in different body fluids of healthy horses, after administrating it orally for 14 days. The study found that the drug distributed widely across the body fluids without showing any significant variance between the 7th and 14th day.

Method and Dosage

• The researchers administered a daily dose of 4 mg/kg of voriconazole to healthy horses. They chose voriconazole as it is considered a very effective medication for many fungal infections.
• The dosing continued for 14 days, and the distribution of the drug in different body fluids was examined on the 7th and 14th day.

Data Collection and Analysis

• The concentration of voriconazole in various body fluids such as plasma, peritoneal, synovial and cerebrospinal fluids, aqueous humor, epithelial lining fluid (ELF), and urine was measured.
• For the accurate quantification of drug concentrations, the researchers employed high-performance liquid chromatography (HPLC), a popular analytical chemistry technique.
• On both the 7th and 14th day, the mean voriconazole concentrations were calculated for each fluid to establish a clear pattern of distribution.

Results

• On the 7th day, the calculated mean concentrations were: plasma (1.47 microg/mL), peritoneal fluid (0.61), synovial fluid (0.70), cerebrospinal fluid (0.62), aqueous humor (0.55), ELF (79.45), and urine (1.83).
• On the 14th day, the concentrations were: plasma (1.60 microg/mL), peritoneal fluid (1.02), synovial fluid (0.86), cerebrospinal fluid (0.64), aqueous humor (0.68), ELF (47.76), and urine (3.34).
• However, the amount of voriconazole in the bronchoalveolar cell pellet, or lung cells, was untraceable.
• There was no statistically significant difference between the concentration of voriconazole in body fluids on the 7th and 14th days, suggesting that the distribution of the drug remained steady over time.

Conclusion

• The work concluded that voriconazole distributes widely into the body’s fluids.
• This broad distribution could be an indicator of the drug’s efficacy and might guide the therapeutic use of voriconazole in battling fungal infections in horses.