Diversity among isolates of Actinobacillus equuli and related organisms as revealed by ribotyping.
Abstract: The objective of this work was to examine the diversity within Australian isolates of Actinobacillus equuli and related organisms by the genotypic method of ribotyping. Methods: Ribotyping, performed using the enzyme HaeIII, was used to examine the diversity in 12 field isolates of A equuli (five being capable of fermenting L-arabinose), one field isolate of Pasteurella caballi and two unclassifiable field isolates. Isolates were obtained from Australian horses, except for three isolates of A equuli (one L-arabinose positive and two L-arabinose negative) which were obtained from horses and a pig in Africa. In addition, the type strains for A equuli and P caballi and a reference strain for Bisgaard Taxon 9 were included in the study. Results: The ribotype patterns were analysed by computerised cluster analysis, yielding five clusters (A to E). All five of the L-arabinose positive A equuli were assigned to cluster A, with all the other seven A equuli isolates (all L-arabinose negative) and the type strain being assigned to cluster B. One of the two unclassified isolates formed cluster C along with the reference strain for Bisgaard Taxon 9. The remaining unclassified isolate formed cluster D. Cluster E consisted of the field isolate and reference strain of P caballi. Conclusions: The results of this study indicate that A equuli is a diverse species, with L-arabinose positive isolates of A equuli being quite distinct from typical L-arabinose negative isolates. Ribotyping appears to be a useful tool in confirming the identity of A equuli-like organisms from horses.
Publication Date: 1998-07-23 PubMed ID: 9673769DOI: 10.1111/j.1751-0813.1998.tb12394.xGoogle Scholar: Lookup
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- Journal Article
Summary
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This research investigation aimed to study the genetic diversity within Australian isolates of a kind of bacteria called Actinobacillus equuli by using a method called ribotyping. The results of the study indicate that A equuli is a diverse species, with different types of A equuli isolates exhibiting diverse characteristics.
Methods and Isolates
- The ribotyping, a genotypic method, was applied using the enzyme HaeIII to evaluate genetic diversity amongst 12 field isolates of A equuli with five being capable of fermenting L-arabinose, a type of sugar.
- One field isolate of Pasteurella caballi and two unidentifiable field isolates were also included in the study. The majority of these isolates were obtained from horses in Australia, with the exception of three isolates of A equuli procured from horses and a pig in Africa.
- In addition, the type strains for A equuli and P caballi and a reference strain for Bisgaard Taxon 9 were included in the study for comprehensive analysis.
Ribotype Analysis and Clustering
- The patterns observed from ribotyping were analysed by computerised cluster analysis. This led to the formation of five distinct clusters ranging from A to E.
- The L-arabinose positive A equuli were assigned to cluster A. The remaining seven A equuli isolates (all of which were L-arabinose negative) were grouped into cluster B along with the type strain.
- The unclassifiable isolates were distributed into clusters C and D. One unclassified isolate, along with the reference strain for Bisgaard Taxon 9, made up cluster C. The remaining unclassified isolate formed cluster D.
- Cluster E included the field isolate and the reference strain of P caballi.
Conclusions and Implications
- The results suggest that there are substantial diversity and distinct differences between L-arabinose positive and negative isolates of A equuli. Hence, A equuli is considered a diverse species.
- Another valuable finding from this study is that ribotyping could be beneficial in confirming the identity of A equuli-like organisms sourced from horses in field conditions.
- This genetic differentiation of isolates could prove significant for epidemiological studies and in understanding the pathogenicity and disease outcomes of different strains of A equuli and related species.
Cite This Article
APA
Blackall PJ, Christensen JP, Bisgaard M.
(1998).
Diversity among isolates of Actinobacillus equuli and related organisms as revealed by ribotyping.
Aust Vet J, 76(6), 423-425.
https://doi.org/10.1111/j.1751-0813.1998.tb12394.x Publication
Researcher Affiliations
- Department of Primary Industries, Animal Research Institute, Yeerongpilly Queensland.
MeSH Terms
- Actinobacillus / classification
- Actinobacillus / genetics
- Actinobacillus Infections / microbiology
- Actinobacillus Infections / veterinary
- Animals
- Australia
- Cluster Analysis
- DNA, Bacterial / analysis
- Deoxyribonucleases, Type II Site-Specific
- Genetic Variation
- Horse Diseases / microbiology
- Horses
- Restriction Mapping
- Swine
- Swine Diseases / microbiology
Citations
This article has been cited 1 times.- Huang BF, Kropinski AM, Bujold AR, MacInnes JI. Complete genome sequence of Actinobacillus equuli subspecies equuli ATCC 19392(T).. Stand Genomic Sci 2015;10:32.
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