Diversity of mitochondrial DNA in three Arabian horse strains.
Abstract: Arabian horse registries classify Arabian horses based on their dam lineages into five main strains. To test the maternal origin of Syrian Arabian horses, 192 horses representing the three major strains Saglawi, Kahlawi, and Hamdani were sequenced for 353 bp of their mitochondrial displacement loop (D-loop) region. Sequencing revealed 28 haplotypes comprising 38 sequence variations. The haplotype diversity values were 0.95, 0.91, and 0.90 in Kahlawi, Hamdani, and Saglawi strains, respectively. The pair-wise population differentiation estimates (Fst) between strains were low, ranging between 0.098 and 0.205. The haplotype diversity and the pair-wise population differentiation estimates (F) between strains showed high diversity within individuals of each strain and low variation between the three strains. Mitochondrial haplotypes scattered all over the neighbor-joining tree without clear separation of the three strains. In the median-joining network, the Syrian horses were grouped into seven major haplogroups. These results suggest that more than five ancestors exist that share common maternal haplotypes with other horse breeds.
Publication Date: 2016-12-14 PubMed ID: 27966062DOI: 10.1007/s13353-016-0384-zGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research study focused on the diversity of mitochondrial DNA among three major strains of Arabian horses, demonstrating a high level of diversity within individual horses, but low variation between the strains.
Objective of Research
- The study aimed to examine the maternal origin of Syrian Arabian horses, specifically the Saglawi, Kahlawi, and Hamdani strains. The researchers used a sample of 192 horses representing these strains, and performed sequencing of a certain part of their mitochondrial DNA known as the D-loop region.
Methodology
- The researchers sequenced 353 base pairs of the horses’ mitochondrial displacement loop (D-loop) region. This region is known for its high mutation frequency and overall variability, making it suitable for population and phylogenetic studies.
- Haplotype diversity values and pair-wise population differentiation estimates (Fst) were calculated to provide insight into how much the horses varied within and across these strains.
Results
- Sequencing revealed 28 different haplotypes resulting from 38 sequence variations amongst the horses studied.
- The haplotype diversity values within each strain ranged from 0.90 to 0.95, indicating a high diversity within individual horses of the same strain.
- The pair-wise population differentiation estimates (Fst) between strains were low, which indicated low variation between different strains.
- In the median-joining network, which is a kind of graph used to summarize relationships between different data points, the Syrian horses were grouped into seven major haplogroups, suggesting a wider range of ancestors than previously thought.
- In the neighbor-joining tree, which is a type of diagram used for illustrating relationships between different species or individuals, the mitochondrial haplotypes of the horses were distributed throughout, without clear separation according to strain.
Conclusions
- The study’s results suggest that the maternal lineage of these Syrian Arabian horse strains shows high diversity within individual horses but low variation between the strains.
- Moreover, the researchers concluded that there’s evidence that these Syrian Arabian horses originated from more than five ancestors, as they shared common maternal haplotypes with other horse breeds.
Cite This Article
APA
Almarzook S, Reissmann M, Brockmann GA.
(2016).
Diversity of mitochondrial DNA in three Arabian horse strains.
J Appl Genet, 58(2), 273-276.
https://doi.org/10.1007/s13353-016-0384-z Publication
Researcher Affiliations
- Albrecht Daniel Thaer-Institute for Agricultural and Horticultural Sciences, Animal Breeding Biology and Molecular Genetics, Humboldt-Universität zu Berlin, Invalidenstrasse 42, 10115, Berlin, Germany.
- Albrecht Daniel Thaer-Institute for Agricultural and Horticultural Sciences, Animal Breeding Biology and Molecular Genetics, Humboldt-Universität zu Berlin, Invalidenstrasse 42, 10115, Berlin, Germany.
- Albrecht Daniel Thaer-Institute for Agricultural and Horticultural Sciences, Animal Breeding Biology and Molecular Genetics, Humboldt-Universität zu Berlin, Invalidenstrasse 42, 10115, Berlin, Germany. gudrun.brockmann@agrar.hu-berlin.de.
MeSH Terms
- Animals
- Breeding
- DNA, Mitochondrial / genetics
- Genetic Variation
- Genetics, Population
- Haplotypes
- Horses / genetics
- Sequence Analysis, DNA / veterinary
- Syria
References
This article includes 13 references
- Lopes MS, Mendonça D, Cymbron T, Valera M, da Costa-Ferreira J, Machado Ada C. The Lusitano horse maternal lineage based on mitochondrial D-loop sequence variation.. Anim Genet 2005 Jun;36(3):196-202.
- Bowling AT, Del Valle A, Bowling M. A pedigree-based study of mitochondrial D-loop DNA sequence variation among Arabian horses.. Anim Genet 2000 Feb;31(1):1-7.
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.. Mol Biol Evol 2011 Oct;28(10):2731-9.
- Cieslak M, Pruvost M, Benecke N, Hofreiter M, Morales A, Reissmann M, Ludwig A. Origin and history of mitochondrial DNA lineages in domestic horses.. PLoS One 2010 Dec 20;5(12):e15311.
- Wallner B, Piumi F, Brem G, Müller M, Achmann R. Isolation of Y chromosome-specific microsatellites in the horse and cross-species amplification in the genus Equus.. J Hered 2004 Mar-Apr;95(2):158-64.
- Kavar T, Brem G, Habe F, Sölkner J, Dovc P. History of Lipizzan horse maternal lines as revealed by mtDNA analysis.. Genet Sel Evol 2002 Sep-Oct;34(5):635-48.
- Keyser-Tracqui C, Blandin-Frappin P, Francfort HP, Ricaut FX, Lepetz S, Crubézy E, Samashev Z, Ludes B. Mitochondrial DNA analysis of horses recovered from a frozen tomb (Berel site, Kazakhstan, 3rd Century BC).. Anim Genet 2005 Jun;36(3):203-9.
- Bandelt HJ, Forster P, Sykes BC, Richards MB. Mitochondrial portraits of human populations using median networks.. Genetics 1995 Oct;141(2):743-53.
- Głazewska I, Wysocka A, Gralak B, Sell J. A new view on dam lines in Polish Arabian horses based on mtDNA analysis.. Genet Sel Evol 2007 Sep-Oct;39(5):609-19.
- Achilli A, Olivieri A, Soares P, Lancioni H, Hooshiar Kashani B, Perego UA, Nergadze SG, Carossa V, Santagostino M, Capomaccio S, Felicetti M, Al-Achkar W, Penedo MC, Verini-Supplizi A, Houshmand M, Woodward SR, Semino O, Silvestrelli M, Giulotto E, Pereira L, Bandelt HJ, Torroni A. Mitochondrial genomes from modern horses reveal the major haplogroups that underwent domestication.. Proc Natl Acad Sci U S A 2012 Feb 14;109(7):2449-54.
- Khanshour AM, Cothran EG. Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing.. BMC Genet 2013 Sep 13;14:83.
- Nei M, Tajima F. DNA polymorphism detectable by restriction endonucleases.. Genetics 1981 Jan;97(1):145-63.
- Excoffier L, Lischer HE. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows.. Mol Ecol Resour 2010 May;10(3):564-7.
Citations
This article has been cited 4 times.- Sheikh A. Mitochondrial DNA sequencing of Kehilan and Hamdani horses from Saudi Arabia.. Saudi J Biol Sci 2023 Sep;30(9):103741.
- Engel L, Becker D, Nissen T, Russ I, Thaller G, Krattenmacher N. Exploring the Origin and Relatedness of Maternal Lineages Through Analysis of Mitochondrial DNA in the Holstein Horse.. Front Genet 2021;12:632500.
- Cosgrove EJ, Sadeghi R, Schlamp F, Holl HM, Moradi-Shahrbabak M, Miraei-Ashtiani SR, Abdalla S, Shykind B, Troedsson M, Stefaniuk-Szmukier M, Prabhu A, Bucca S, Bugno-Poniewierska M, Wallner B, Malek J, Miller DC, Clark AG, Antczak DF, Brooks SA. Genome Diversity and the Origin of the Arabian Horse.. Sci Rep 2020 Jun 16;10(1):9702.
- Sadeghi R, Moradi-Shahrbabak M, Miraei Ashtiani SR, Miller DC, Antczak DF. MHC haplotype diversity in Persian Arabian horses determined using polymorphic microsatellites.. Immunogenetics 2018 May;70(5):305-315.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
