DNA barcoding of Anoplocephala perfoliata derived from a draft horse (Ban’ei horse) in Hokkaido, Japan.
Abstract: A two-year-old male Japanese draft horse (known as a "Ban'ei horse") excreted eight cestodes. Based on their morphological features, they were identified as Anoplocephala perfoliata. The partial mitochondrial cytochrome c oxidase subunit 1 (COI) sequences of the worms were nearly identical to A. perfoliata isolated from horses in Europe. The results of phylogenetic analyses of COI revealed that our samples and the European isolates formed the same clade, which was separate from Chinese and Australian isolates. Ban'ei horses were developed by crossbreeding draft horses imported from European countries in the 1900s. Our results suggest that A. perfoliata was transported to Hokkaido with horses from Europe. To our knowledge, this is the first report of A. perfoliata infection in a Japanese draft horse.
©2024 The Japanese Society of Equine Science.
Publication Date: 2024-10-11 PubMed ID: 39411212PubMed Central: PMC11473121DOI: 10.1294/jes.35.43Google Scholar: Lookup
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- Journal Article
Summary
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Overview
- This study identified the tapeworm Anoplocephala perfoliata in a Japanese draft horse and confirmed its genetic similarity to European strains, suggesting the parasite was introduced to Japan with European horses.
Introduction and Context
- The research focused on a two-year-old male Japanese draft horse, known as a Ban’ei horse, located in Hokkaido, Japan.
- The Ban’ei horse breed originated from crossbreeding draft horses imported from Europe in the early 1900s.
- Anoplocephala perfoliata is a species of tapeworm commonly found in horses that can affect equine health.
Methodology
- Eight tapeworms were collected from the feces of the Ban’ei horse.
- Identification was initially based on morphological features, confirming these cestodes as Anoplocephala perfoliata.
- Genetic analysis was done by sequencing a part of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene from the worms.
- Phylogenetic analyses were performed on the COI sequences to determine the evolutionary relationships between the Japanese worms and others globally.
Results
- The COI sequences from the Japanese worms were nearly identical to those of A. perfoliata isolated from horses in Europe.
- Phylogenetic trees showed that the Japanese samples clustered with European isolates, forming a distinct clade separate from isolates found in China and Australia.
- This genetic relationship indicates that the parasites in the Ban’ei horse share a recent common ancestry with European strains.
Significance and Implications
- The study suggests that Anoplocephala perfoliata was likely introduced to Hokkaido through the importation of European draft horses during the breed development of Ban’ei horses.
- This is the first documented report of A. perfoliata infection in a Japanese draft horse, expanding knowledge on equine parasitic infections in Japan.
- Understanding the origin and genetic relationships of parasites helps in managing equine health and preventing the spread of these parasites through international horse transport.
Conclusion
- The research combines morphological identification and genetic barcoding (COI sequencing) to confirm the presence of A. perfoliata in a Ban’ei horse.
- Phylogenetic analysis supports the hypothesis that this parasite was introduced into Japan by imported European horses.
- The study contributes valuable genetic data on A. perfoliata and informs disease management in equine populations in Japan and beyond.
Cite This Article
APA
Sasaki M, Fukumoto N, Fukumoto S.
(2024).
DNA barcoding of Anoplocephala perfoliata derived from a draft horse (Ban’ei horse) in Hokkaido, Japan.
J Equine Sci, 35(3), 43-46.
https://doi.org/10.1294/jes.35.43 Publication
Researcher Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
- Athena Integrative Veterinary Care, Hokkaido 080-0023, Japan.
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
- Athena Integrative Veterinary Care, Hokkaido 080-0023, Japan.
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Citations
This article has been cited 1 times.- Coronado-Morones D, Alonso Panti-May J, Torres-Carrera G, Garcia-Prieto L. Description of a new Neotropical species of Mathevotaenia (Cestoda: Anoplocephalidae), a parasite of the Virginia opossum Didelphis virginiana.. Folia Parasitol 2025 Jul 23;72.
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