Omneity® Pellets
All-In-One Vitamin & Mineral Pellet
DNA fragmentation of equine cumulus cells from Cumulus-Oocyte complexes submitted to vitrification and its relationship to the developmental competence of the oocyte.
Abstract: The objectives of this study were to evaluate the effect of vitrification on the DNA fragmentation rate of equine cumulus cells and to assess its relationship to oocyte in vitro maturation (IVM) after vitrification. Cumulus cells (CC) from 14 mares were recovered from COCs, previously submitted to vitrification (VIT) and IVM. The DNA fragmentation rate of the cumulus cells (CC-DF) was assessed using a chromatin dispersion test. CC-DF rates between vitrified and control COCs were statistically compared by Student's t-test. The rates of CC-DF from control COCs were lower than in vitrified COCs. The percentage of CC-DF was not significantly different (p > .05) between groups of COCs able to reach metaphase II (MII > 0) and those in which oocyte maturation was not achieved (MII = 0). In conclusion, vitrification has a deleterious effect on the DNA fragmentation of equine cumulus cells; however, this parameter cannot be used as a predictor for IVM success after COCs vitrification.
© 2022 The Authors. Reproduction in Domestic Animals published by Wiley-VCH GmbH.
Publication Date: 2022-07-08 PubMed ID: 35770452PubMed Central: PMC9795872DOI: 10.1111/rda.14197Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research studied the impact of a freezing process called vitrification on the DNA damage rate in horse cumulus cells and how this damage might affect the success of in vitro egg maturation. The study found that vitrification increases DNA damage but it does not necessarily hinder the success of in vitro egg maturation.
Methods of the Investigation
The study focused on the following points:
- Cumulus cells obtained from 14 horses were analyzed, these cells were part of Cumulus-Oocyte complexes (COCs), which are clusters of cells that surround an egg in the ovaries.
- These COCs underwent a process known as vitrification, which is a type of freezing that can be used to preserve eggs or embryos for potential future use in assisted reproductive practices.
- In vitro maturation (IVM) is a technique that allows eggs to be matured outside the body, in a laboratory setting. This process was applied to the COCs after they had been vitrified.
- The DNA fragmentation rate of the cumulus cells, meaning the measure of DNA damage, was then measured using a technique called a chromatin dispersion test.
- This rate was compared statistically between the cumulus cells that were vitrified and a control group of cumulus cells.
Key Findings of the Study
The research led to several important discoveries:
- The DNA fragmentation rates were higher in the vitrified COCs compared to the control group, indicating that the vitrification process causes an increased level of DNA damage.
- The likelihood of achieving successful oocyte maturation did not significantly differ between groups with different levels of DNA fragmentation. In other words, the success of IVM was not necessarily affected by the rates of DNA damage.
- Despite the increased DNA damage caused by vitrification, this factor could not be used as a reliable predictor of successful IVM after the COCs have undergone vitrification.
This study underlines the importance of further research to identify parameter(s) that could accurately predict the successful maturation of eggs after vitrification process.
Cite This Article
APA
Ortiz I, Dorado J, Pereira B, Diaz-Jimenez M, Consuegra C, Gosalvez J, Hidalgo M.
(2022).
DNA fragmentation of equine cumulus cells from Cumulus-Oocyte complexes submitted to vitrification and its relationship to the developmental competence of the oocyte.
Reprod Domest Anim, 57 Suppl 5(Suppl 5), 64-67.
https://doi.org/10.1111/rda.14197 Publication
Researcher Affiliations
- Department of Animal Medicine and Surgery, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Department of Animal Medicine and Surgery, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Department of Animal Medicine and Surgery, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Department of Animal Medicine and Surgery, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Department of Animal Medicine and Surgery, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Department of Biology, Autonomous University of Madrid, Madrid, Spain.
- Department of Animal Medicine and Surgery, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
MeSH Terms
- Animals
- Chromatin
- Cryopreservation / veterinary
- Cumulus Cells
- DNA Fragmentation
- Female
- Horses
- In Vitro Oocyte Maturation Techniques / veterinary
- Oocytes
- Vitrification
Grant Funding
- Ministerio de Economu00eda y Competitividad
- DOC_00399 / European Social Fund 2014-2020
Conflict of Interest Statement
The authors have no conflict of interest to declare.
References
This article includes 14 references
- Angel-Velez D, De Coster T, Azari-Dolatabad N, Fernandez-Montoro A, Benedetti C, Bogado Pascottini O, Woelders H, Van Soom A, Smits K. New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification.. Animals (Basel) 2021 Oct 28;11(11).
- Barcena P, López-Fernández C, García-Ochoa C, Obradors A, Vernaeve V, Gosálvez J, Vassena R. Detection of DNA damage in cumulus cells using a chromatin dispersion assay.. Syst Biol Reprod Med 2015;61(5):277-85.
- Canesin HS, Brom-de-Luna JG, Choi YH, Ortiz I, Diaw M, Hinrichs K. Blastocyst development after intracytoplasmic sperm injection of equine oocytes vitrified at the germinal-vesicle stage.. Cryobiology 2017 Apr;75:52-59.
- Dell'Aquila ME, Albrizio M, Maritato F, Minoia P, Hinrichs K. Meiotic competence of equine oocytes and pronucleus formation after intracytoplasmic sperm injection (ICSI) as related to granulosa cell apoptosis.. Biol Reprod 2003 Jun;68(6):2065-72.
- Hinrichs K. The equine oocyte: factors affecting meiotic and developmental competence.. Mol Reprod Dev 2010 Aug;77(8):651-61.
- Hinrichs K. Assisted reproductive techniques in mares.. Reprod Domest Anim 2018 Sep;53 Suppl 2:4-13.
- Jewgenow K, Heerdegen B, Müller K. In vitro development of individually matured bovine oocytes in relation to follicular wall atresia.. Theriogenology 1999 Mar;51(4):745-56.
- Jin B, Mazur P. High survival of mouse oocytes/embryos after vitrification without permeating cryoprotectants followed by ultra-rapid warming with an IR laser pulse.. Sci Rep 2015 Mar 19;5:9271.
- Maclellan LJ, Carnevale EM, Coutinho da Silva MA, Scoggin CF, Bruemmer JE, Squires EL. Pregnancies from vitrified equine oocytes collected from super-stimulated and non-stimulated mares.. Theriogenology 2002 Sep;58(5):911-9.
- Ortiz-Escribano N, Bogado Pascottini O, Woelders H, Vandenberghe L, De Schauwer C, Govaere J, Van den Abbeel E, Vullers T, Ververs C, Roels K, Van De Velde M, Van Soom A, Smits K. An improved vitrification protocol for equine immature oocytes, resulting in a first live foal.. Equine Vet J 2018 May;50(3):391-397.
- Pereira B, Dorado J, Diaz-Jimenez M, Consuegra C, Ortiz I, Gosalvez J, Hidalgo M. Relationship between DNA fragmentation of equine granulosa cells and oocyte meiotic competence after in vitro maturation.. Reprod Domest Anim 2019 Oct;54 Suppl 4:78-81.
- Pereira BC, Ortiz I, Dorado JM, Diaz-Jimenez MA, Consuegra C, Gosalvez J, Hidalgo M. Effect of permeable cryoprotectant-free vitrification on DNA fragmentation of equine oocyte-cumulus cells.. Reprod Domest Anim 2019 Sep;54 Suppl 3:53-56.
- Ribeiro BI, Love LB, Choi YH, Hinrichs K. Transport of equine ovaries for assisted reproduction.. Anim Reprod Sci 2008 Oct;108(1-2):171-9.
- Tharasanit T, Colleoni S, Lazzari G, Colenbrander B, Galli C, Stout TA. Effect of cumulus morphology and maturation stage on the cryopreservability of equine oocytes.. Reproduction 2006 Nov;132(5):759-69.
Citations
This article has been cited 1 times.- Yurchuk T, Likszo P, Witek K, Petrushko M, Skarzynski DJ. New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model. Int J Mol Sci 2024 May 31;25(11).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
