DNA Methylation and Its Effects on TRIM29 Gene Expression in the Equine Sarcoid Tissue.
Abstract: Sarcoids are the most frequently diagnosed dermatological tumour in horses. It is a disease that can affect various species of equids, such as donkeys, mules and zebras. This type of tumour can develop in all horse breeds, regardless of age and gender. Treatment options depend on many factors, such as the type of lesion, location, extent, owner preference and financial considerations. In the present study, we investigated the TRIM29 expression, the methylation status of its first exon and its involvement in the formation of equine sarcoids. Bisulfite sequencing PCR (BSP) was used to determine DNA methylation at CpG sites and real-time quantitative polymerase chain reaction (qPCR) was used to detect TRIM29 expression level. Our results showed that TRIM29 is significantly downregulated in lesional samples (FC = -3.72; p < 0.001). Furthermore, TRIM29 expression was significantly correlated (R = -0.73; p < 0.001) with hypermethylation of its specific CpG sites in the first exon of this gene. Our research has demonstrated that the identification of increased methylation of CpG sequences in horse sarcoids, along with the decreased expression of the TRIM29 gene, is an important step towards understanding the molecular mechanisms underlying the disease. These findings can serve in the future as a diagnostic biomarker for horse sarcoids and help in detecting the disease.
© 2024 John Wiley & Sons Ltd.
Publication Date: 2024-07-10 PubMed ID: 38984644DOI: 10.1111/vco.12994Google Scholar: Lookup
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Summary
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This research article explores the relationship between DNA methylation and the expression of the TRIM29 gene in equine sarcoid tumours. It is found that hypermethylation of the gene correlates with reduced expression, which could potentially serve as a diagnostic biomarker for the disease.
Introduction
- Sarcoids are common dermatological tumours in horses and can affect a variety of equid species such as donkeys, mules, and zebras. These tumours can occur in horses of any breed, age, or sex, with treatments varying depending on factors such as the lesion type, its location, the extent, owner preference, and financial factors.
Research Methodology
- The study investigated the TRIM29 gene expression, the methylation status of its first exon and its potential involvement in the formation of equine sarcoids.
- Bisulfite sequencing PCR (BSP) was used to determine DNA methylation at CpG sites. Methylation status has been identified as an important regulatory mechanism that can influence gene expression.
- The researchers also used real-time quantitative polymerase chain reaction (qPCR) to detect the levels of TRIM29 gene expression.
Results
- The outcome showed that the expression of TRIM29 was significantly downregulated in samples taken from lesions, with a Fold Change of -3.72, suggesting altered regulation of this gene in sarcoid conditions.
- The analysis also revealed a significant correlation between TRIM29 expression and hypermethylation of its specific CpG sites in the first exon of the gene.
Conclusion
- The increased methylation of CpG sequences along with decreased TRIM29 gene expression indicates a key step towards understanding the molecular mechanisms that contribute to the development of horse sarcoids.
- This finding could potentially be utilized as a diagnostic biomarker in the future for detecting horse sarcoids, thus improving treatment options and outcomes.
Cite This Article
APA
Ewelina SG, Anna Z, Rafał P, Maciej W.
(2024).
DNA Methylation and Its Effects on TRIM29 Gene Expression in the Equine Sarcoid Tissue.
Vet Comp Oncol.
https://doi.org/10.1111/vco.12994 Publication
Researcher Affiliations
- Department of Animal Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
- University of Agriculture in Krakow, Krakow, Poland.
- University Centre of Veterinary Medicine JU-UA, University of Agriculture in Krakow, Krakow, Poland.
- Equine Vet Clinic EQUI-VET, Stogniowice, Poland.
- University Centre of Veterinary Medicine JU-UA, University of Agriculture in Krakow, Krakow, Poland.
- Equine Hospital on the Racing Truck, Warszawa, Poland.
Grant Funding
- National Research Institute of Animal Production
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