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Home / Equine Research Bank / Arch Virol / DNA of bovine papillomavirus type 1 and 2 in equine sarcoids...
Archives of virology1993; 132(1-2); 121-131; doi: 10.1007/BF01309847

DNA of bovine papillomavirus type 1 and 2 in equine sarcoids: PCR detection and direct sequencing.

Abstract: Nucleotide sequences of bovine papillomavirus (BPV) DNA amplified by the polymerase chain reaction (PCR) from samples of equine sarcoid skin tumours were determined. All naturally occurring sarcoids (n = 58 tumours from 32 horses and 2 donkeys) contained BPV-DNA. All but 3 of the genome fragments belonged to the BPV type 1 strain (BPV-1); the remaining were BPV type 2. Similar results were obtained with cutaneous bovine papillomas used as controls (n = 20). One of the horses, carrying 2 sarcoids, was particularly interesting; one tumour contained BPV-1 DNA whilst the other sarcoid yielded BPV-2 DNA, suggesting that horses are not immune to super-infection. BPV-DNA was even amplified from the sarcoid samples which had yielded negative results in previous investigations when DNA isolated from the lesions was used in Southern blot hybridization with BPV probes. In addition, there was no detectable BPV-DNA in any equine or bovine tissue examined other than sarcoids or cutaneous bovine papillomas. Biopsies of normal skin surrounding lesions yielded exclusively negative results. The described nucleotide differences represent a natural genomic variation of this BPV type between geographically distant locations. The identical variations recovered from cattle and horses in Switzerland, a finding of great epidemiological interest, strongly suggest that a uniform variant of BPV-1 is one of the etiologic agents of equine sarcoid and bovine papilloma in a given region.
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Publication Date: 1993-01-01 PubMed ID: 8394687DOI: 10.1007/BF01309847Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the occurrence and type of bovine papillomavirus (BPV) in equine skin tumors called sarcoids. The study utilizes Polymerase Chain Reaction (PCR) to determine the DNA sequences of BPV present in the tumors.

Methodology and Findings

  • The study employed the Polymerase Chain Reaction (PCR) technique for amplifying BPV DNA obtained from samples of equine sarcoid skin tumors.
  • Out of 58 skin tumors from 32 horses and 2 donkeys analyzed, all were found to contain BPV DNA. Majority of the genome fragments (except for 3) were identified as BPV type 1 strain (BPV-1). The remaining were of BPV type 2.
  • Cutaneous bovine papillomas, used as controls, produced similar results with 20 samples analyzed.
  • In a unique case, one horse with two sarcoids demonstrated different strains of BPV in each, suggesting that horses can be susceptible to multiple infections.

Additional Observations

  • The study found BPV DNA even from sarcoid samples that had previously tested negative for BPV DNA when the DNA was isolated from lesions and used in Southern blot hybridization along with BPV probes.
  • No detectable BPV DNA was found in any other equine or bovine tissue besides sarcoids or cutaneous bovine papillomas.
  • Biopsies from normal skin surrounding the lesions showed no trace of BPV DNA, indicating a localized presence in the sarcoids and bovine papillomas.

Genomic Variation and Epidemiological Interest

  • The genomic differences underscore a varying natural presence of this BPV type in different geographical locations.
  • Considering similar genomic variations found in cattle and horses from Switzerland, it was inferred that a specific variant of BPV-1 might be a common causative agent for both equine sarcoid and bovine papilloma in certain regions. This discovery holds significant epidemiological implications.

Cite This Article

APA
Otten N, von Tscharner C, Lazary S, Antczak DF, Gerber H. (1993). DNA of bovine papillomavirus type 1 and 2 in equine sarcoids: PCR detection and direct sequencing. Arch Virol, 132(1-2), 121-131. https://doi.org/10.1007/BF01309847

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 132
Issue: 1-2
Pages: 121-131

Researcher Affiliations

Otten, N
  • Klinik für Nutztiere und Pferde, University of Berne, Switzerland.
von Tscharner, C
    Lazary, S
      Antczak, D F
        Gerber, H

          MeSH Terms

          • Animals
          • Base Sequence
          • Bovine papillomavirus 1 / genetics
          • Cattle
          • DNA, Viral / analysis
          • DNA, Viral / genetics
          • Horse Diseases / microbiology
          • Horses
          • Molecular Sequence Data
          • Papilloma / microbiology
          • Papilloma / veterinary
          • Polymerase Chain Reaction / veterinary
          • Skin Neoplasms / microbiology
          • Skin Neoplasms / veterinary
          • Warts / microbiology
          • Warts / veterinary

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