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Home / Equine Research Bank / Virology / DNA sequence and comparative analyses of the equine herpesvi...
Virology1989; 172(1); 223-236; doi: 10.1016/0042-6822(89)90124-4

DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene.

Abstract: The immediate early (IE) proteins of herpesviruses are important regulatory factors which control the expression of genes at the transcriptional level. We report the DNA sequence of the immediate early gene of the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This sequence is shown to be extremely rich in guanine and cytosine, resulting in a highly biased codon usage. The IE gene region possesses 38 open reading frames (ORFs) greater than 300 bp in length, 11 of which have coding regions of at least 100 amino acids (aa) following potential translation initiator codons. The largest ORF consists of 1487 codons (4461 bp) starting with the first ATG and would encode a protein of MW 155,000. TATA and CCAAT sequences as well as several potential cis-acting elements lie upstream to the major ORF. The deduced amino acid sequence for the 155,000 protein has a high degree of homology to the herpes simplex virus type 1 (HSV-1) ICP4 protein and its varicella-zoster virus (VZV) homolog. The regions of the EHV-1 IE protein that are homologous with these proteins correspond to the previously determined pattern of homology between the HSV and VZV IE polypeptides. However, there are are a number of differences within these broadly defined regions. It is therefore expected that this comparative study will facilitate the identification of functionally important residues within the amino acid sequence of IE proteins.
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Publication Date: 1989-09-01 PubMed ID: 2549711DOI: 10.1016/0042-6822(89)90124-4Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on studying the immediate early (IE) gene of equine herpesvirus type 1 (EHV-1), deciphering its DNA sequence, and comparing its amino acid sequence with similar genes in other herpesvirus types.

Decoding the DNA Sequence of the IE gene of EHV-1

  • The IE proteins of herpesviruses are crucial regulatory factors that control gene expression at the transcription level. The DNA sequence of the EHV-1’s IE gene was revealed in this study, which was found to be rich in guanine and cytosine, leading to a highly biased codon usage.
  • The IE gene region was shown to have 38 open reading frames (ORFs) with over 300 base pairs in length, with 11 of these having coding regions of at least 100 amino acids following potential translation starting points.
  • The largest ORF had 1487 codons or 4461 base pairs, which initiated at the first ATG and encoded a protein of 155,000 molecular weight (MW).
  • Notably, TATA and CCAAT sequences, along with several potential cis-acting elements were discovered upstream to the major ORF.

Comparative Analysis of the Deduced Amino Acid Sequence

  • The deciphered amino acid sequence for the 155,000 MW protein showed high similarity to the herpes simplex virus type 1 (HSV-1) ICP4 protein and its varicella-zoster virus (VZV) variant.
  • The sections of the EHV-1 IE protein that corresponded with these proteins matched the previously established pattern of similarity between the HSV and VZV IE polypeptides.
  • However, several differences were also found within these broad regions. These differences are significant as they could help identify functionally important residues within the amino acid sequence of IE proteins, which would ultimately enhance the understanding of the herpesvirus biology.

Cite This Article

APA
Grundy FJ, Baumann RP, O'Callaghan DJ. (1989). DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene. Virology, 172(1), 223-236. https://doi.org/10.1016/0042-6822(89)90124-4

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 172
Issue: 1
Pages: 223-236

Researcher Affiliations

Grundy, F J
  • Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932.
Baumann, R P
    O'Callaghan, D J

      MeSH Terms

      • Amino Acid Sequence
      • Base Sequence
      • Codon
      • DNA, Viral / genetics
      • Genes, Viral
      • Herpesviridae / genetics
      • Herpesvirus 1, Equid / genetics
      • Immediate-Early Proteins
      • Molecular Sequence Data
      • Poly A / genetics
      • Regulatory Sequences, Nucleic Acid
      • Viral Proteins / genetics

      Grant Funding

      • AI22001 / NIAID NIH HHS

      Citations

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