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Reproduction in domestic animals = Zuchthygiene2024; 59(9); e14718; doi: 10.1111/rda.14718

Does the Uterine Ozone Therapy Alter the Transcript Profile of Anti- and Proinflammatory Genes in Mares With Endometritis?

Abstract: This study aimed to evaluate the localised effects of intrauterine ozone therapy on endometrial recovery in mares with endometritis. Our investigation assessed changes in gene expression profiles of anti-inflammatory (IL-1RA and IL-10), proinflammatory (IL-R1B3i and TNFα) and pleiotropic (IL-6) cytokines, along with detailed histological measurements of epithelial and endometrial thickness and the glandular area ratio. Twenty mares were assigned to a 2 × 2 factorial design based on endometritis diagnosis and treatment (control or 42 μg/mL ozone insufflation), resulting in four groups: NC (negative for endometritis/control), NO (negative/ozone), PC (positive/control) and PO (positive/ozone). Oestrus was induced with 2 mg of oestradiol benzoate on Days -1, 1 and 3, plus 1 mg on Day 5. Day 0 marked the initial uterine treatment, followed by insufflations on Days 1 and 2 with O (ozone) or O (control). Uterine biopsies were taken before treatment on Day 0 and Day 6 for histological analysis and gene expression assessment. Data were analysed using a statistical model that included endometritis status, treatment type, biopsy times (D0 and D6) and their interactions, analysed with Proc Glimmix. Regardless of treatment or endometritis status, significant biopsy effects (p < 0.01) indicated increased epithelial height and endometrial thickness in Day 6 samples. Analysis of IL-1 and TNFα revealed a significant interaction (p < 0.05) among endometritis, treatment and biopsy, with higher IL-1B3i expression on Day 6 in the PC group. The treatment effect (p < 0.04) showed a higher frequency (p < 0.01) of animals with positive modulation in the PC group (66.7%) versus the PO group (0.0%). An interaction effect (p = 0.08) between endometritis and treatment resulted from higher IL-1RA expression on Day 6 in the PC group compared to the PO group. Biopsy effect was significant for IL-10 (p < 0.01), indicating higher values in the second sample associated with tissue repair. In the short-term evaluation, ozone therapy did not influence endometrial morphology and may modulate cytokine expression, specifically the reduction in IL-1 and TNFα levels. Therefore, this therapy appears to be a safe and potentially effective treatment for modulating the inflammatory response in mares with endometritis.
© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.
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Publication Date: 2024-09-10 PubMed ID: 39253801DOI: 10.1111/rda.14718Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the effects of intrauterine ozone therapy on the recovery of the endometrium in mares (female horses) with endometritis, an inflammation of the inner lining of the uterus. The study monitors changes in the expression of certain cytokines, along with changes in epithelial and endometrial thickness. The resulting data suggest that ozone therapy may have potential as a safe and effective treatment for modulating the inflammatory response in mares with endometritis.

Research Design

  • The study involved 20 mares that were divided into four groups based on diagnosis of endometritis and nature of treatment (control or ozone therapy).
  • Over a period of six days, the mares were induced with oestrus and were given intrauterine treatments. The days were marked from 0 to 6, with day 0 being the initial treatment day and biopsies were taken before treatment on Day 0 and Day 6.
  • The factors considered in the statistical model the researchers utilized include endometritis status, treatment type, and biopsy times.

Findings

  • The results showed significant effects of biopsy, with an increase in epithelial height and endometrial thickness in samples taken on Day 6, regardless of treatment or the presence of endometritis.
  • Analysis of cytokines indicated an interaction among endometritis status, treatment type, and biopsy. The expression of certain cytokines (IL-1 and TNFα) was higher in the group diagnosed with endometritis and left untreated, particularly on Day 6.
  • The frequency of animals with positive modulation was higher in the untreated endometritis group versus the group with endometritis that received ozone therapy.
  • The study also observed higher expression of certain cytokine (IL-1RA) in the untreated endometritis group compared to the treated group.
  • Another cytokine (IL-10), often associated with tissue repair, was found in higher quantities in the second sample, indicating its presence during the recovery process.

Conclusions

  • The study suggests that ozone therapy may be a potentially effective treatment for controlling the inflammatory response in mares with endometritis, as the therapy did not appear to negatively affect endometrial morphology and appeared to lower levels of proinflammatory cytokines.

Cite This Article

APA
Botelho JHV, Pessoa GO, Caixeta ES, Sales G, de Oliveira KR, Nascimento Neto JDP, Ferreira RD, Palhão MP. (2024). Does the Uterine Ozone Therapy Alter the Transcript Profile of Anti- and Proinflammatory Genes in Mares With Endometritis? Reprod Domest Anim, 59(9), e14718. https://doi.org/10.1111/rda.14718

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 59
Issue: 9
Pages: e14718

Researcher Affiliations

Botelho, Jorge Henrique Villela
  • Universidade Professor Edson Antônio Velano, Alfenas, Minas Gerais, Brazil.
Pessoa, Gabriela Oliveira
  • Federal University of Lavras-UFLA, Lavras, Minas Gerais, Brazil.
Caixeta, Ester Siqueira
  • Federal University of Alfenas-UNIFAL, Alfenas, Minas Gerais, Brazil.
Sales, Giuliana
  • Federal University of Lavras-UFLA, Lavras, Minas Gerais, Brazil.
de Oliveira, Karine Rabelo
  • Federal University of Lavras-UFLA, Lavras, Minas Gerais, Brazil.
Nascimento Neto, José da Páscoa
  • Federal University of Lavras-UFLA, Lavras, Minas Gerais, Brazil.
Ferreira, Roger Dinali
  • Federal University of Lavras-UFLA, Lavras, Minas Gerais, Brazil.
Palhão, Miller Pereira
  • Universidade Professor Edson Antônio Velano, Alfenas, Minas Gerais, Brazil.

MeSH Terms

  • Animals
  • Female
  • Ozone / pharmacology
  • Endometritis / veterinary
  • Endometritis / drug therapy
  • Horses
  • Horse Diseases / drug therapy
  • Uterus / pathology
  • Cytokines / genetics
  • Cytokines / metabolism
  • Endometrium / drug effects
  • Endometrium / metabolism
  • Endometrium / pathology
  • Estradiol / pharmacology
  • Estradiol / analogs & derivatives
  • Transcriptome

Grant Funding

  • Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico
  • Fundau00e7u00e3o de Amparo u00e0 Pesquisa do Estado de Minas Gerais

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