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Home / Equine Research Bank / J Vet Med Sci / Dominant obligate anaerobes revealed in lower respiratory tr...
The Journal of veterinary medical science2013; 76(4); 587-591; doi: 10.1292/jvms.13-0272

Dominant obligate anaerobes revealed in lower respiratory tract infection in horses by 16S rRNA gene sequencing.

Abstract: Obligate anaerobes are important etiological agents in pneumonia or pleuropneumonia in horses, because they are isolated more commonly from ill horses that have died or been euthanized than from those that survive. We performed bacterial identification and antimicrobial susceptibility testing for obligate anaerobes to establish effective antimicrobial therapy. We used 16S rRNA gene sequencing to identify 58 obligate anaerobes and compared the results with those from a phenotypic identification kit. The identification results of 16S rRNA gene sequencing were more reliable than those of the commercial kit. We concluded that genera Bacteroides and Prevotella-especially B. fragilis and P. heparinolytica-are dominant anaerobes in lower respiratory tract infection in horses; these organisms were susceptible to metronidazole, imipenem and clindamycin.
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Publication Date: 2013-12-20 PubMed ID: 24366152PubMed Central: PMC4064148DOI: 10.1292/jvms.13-0272Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This article investigates the types of bacteria causing respiratory infections in horses by analyzing their 16S rRNA genes. The researchers found that the bacteria Bacteroides and Prevotella were the main culprits and were susceptible to the antibiotics metronidazole, imipenem, and clindamycin.

Research Objective

  • The main aim of this study was to identify the bacteria species (obligate anaerobes) that play a critical role in causing pneumonia or pleuropneumonia in horses. Furthermore, the study intended to test for the sensitivity of these bacteria to certain antibiotics to inform effective treatment strategies.

Methodology

  • The researchers utilized 16S rRNA gene sequencing as a method to identify the bacteria present in the lower respiratory tract of the infected horses.
  • A total of 58 obligate anaerobes were identified and through this method and results were compared with those obtained from a commercial identification kit.

Findings

  • The 16S rRNA gene sequencing was found to be more reliable as compared to the commercial identification kit in correctly identifying the bacteria involved.
  • The bacteria belonging to the genera Bacteroides and Prevotella, specifically B. fragilis and P. heparinolytica, were identified as dominant anaerobes in lower respiratory tract infections in horses.

Antibiotic Sensitivity

  • The identified Bacteroides and Prevotella were found to be sensitive to a number of antibiotics including metronidazole, imipenem, and clindamycin.
  • This finding suggests that these antibiotics could be used effectively to treat horses suffering from pneumonia or pleuropneumonia caused by the above-mentioned bacteria.

Cite This Article

APA
Kinoshita Y, Niwa H, Katayama Y, Hariu K. (2013). Dominant obligate anaerobes revealed in lower respiratory tract infection in horses by 16S rRNA gene sequencing. J Vet Med Sci, 76(4), 587-591. https://doi.org/10.1292/jvms.13-0272

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 76
Issue: 4
Pages: 587-591

Researcher Affiliations

Kinoshita, Yuta
  • Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotuke, Tochigi 329-0412, Japan.
Niwa, Hidekazu
    Katayama, Yoshinari
      Hariu, Kazuhisa

        MeSH Terms

        • Animals
        • Anti-Infective Agents / pharmacology
        • Bacteria, Anaerobic / drug effects
        • Bacteria, Anaerobic / genetics
        • Bacteroides / drug effects
        • Bacteroides / genetics
        • Base Sequence
        • Bronchoalveolar Lavage Fluid / microbiology
        • Clindamycin
        • Cluster Analysis
        • Horse Diseases / microbiology
        • Horses
        • Imipenem
        • Metronidazole
        • Molecular Sequence Data
        • Phylogeny
        • Prevotella / drug effects
        • Prevotella / genetics
        • RNA, Ribosomal, 16S / genetics
        • Respiratory Tract Infections / microbiology
        • Respiratory Tract Infections / veterinary
        • Sequence Analysis, DNA
        • Species Specificity

        References

        This article includes 26 references
        1. Adderson E. E., Boudreaux J. W., Cummings J. R., Pounds S., Wilson D. A., Procop G. W., Hayden R. T.. Identification of clinical coryneform bacterial isolates: comparison of biochemical methods and sequence analysis of 16S rRNA and rpoB genes. J. Clin. Microbiol. 46: 921–927.
          doi: 10.1128/JCM.01849-07pmc: PMC2268328pubmed: 18160450google scholar: lookup
        2. Alexander C. J., Citron D. M., Hunt Gerardo S., Claros M. C., Talan D., Goldstein E. J.. Characterization of saccharolytic Bacteroides and Prevotella isolates from infected dog and cat bite wounds in humans. J. Clin. Microbiol. 35: 406–411.
          pmc: PMC229590pubmed: 9003606
        3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. Basic local alignment search tool. J. Mol. Biol. 215: 403–410.
          pubmed: 2231712
        4. Blunden A. S., Mackintosh M. E.. The microflora of the lower respiratory tract of the horse: an autopsy study. Br. Vet. J. 147: 238–250.
          doi: 10.1016/0007-1935(91)90048-Rpubmed: 1878768google scholar: lookup
        5. Boyanova L., Kolarov R., Gergova G., Dimitrova L., Mitov I.. Trends in antibiotic resistance in Prevotella species from patients of the University Hospital of Maxillofacial Surgery, Sofia, Bulgaria, in 2003–2009. Anaerobe 16: 489–492.
          doi: 10.1016/j.anaerobe.2010.07.004pubmed: 20670687google scholar: lookup
        6. Cai H., Archambault M., Prescott J. F.. 16S ribosomal RNA sequence-based identification of veterinary clinical bacteria. J. Vet. Diagn. Invest. 15: 465–469.
          doi: 10.1177/104063870301500511pubmed: 14535548google scholar: lookup
        7. Carlson G. P., O’Brien M. A.. Anaerobic bacterial pneumonia with septicemia in two racehorses. J. Am. Vet. Med. Assoc. 196: 941–943.
          pubmed: 2312393
        8. Clinical and Laboratory Standards Institute. Methods for antimicrobial susceptibility testing of anaerobic bacteria, Approved standard M11-A7. 2007.
          pubmed: 31339681
        9. Clinical and Laboratory Standards Institute. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing; Approved guideline MM18-A. 2008.
        10. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement M100-S22. 2012.
        11. Cloud J. L., Harmsen D., Iwen P. C., Dunn J. J., Hall G., Lasala P. R., Hoggan K., Wilson D., Woods G. L., Mellmann A.. Comparison of traditional phenotypic identification methods with partial 5′ 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli. J. Clin. Microbiol. 48: 1442–1444.
          doi: 10.1128/JCM.00169-10pmc: PMC2849612pubmed: 20164273google scholar: lookup
        12. Foster T.. Staphylococcus. 1996.
          pubmed: 21413338
        13. Frederick J., Giguere S., Sanchez L. C.. Infectious agents detected in the feces of diarrheic foals: a retrospective study of 233 cases (2003–2008). J. Vet. Intern. Med. 23: 1254–1260.
          doi: 10.1111/j.1939-1676.2009.0383.xpmc: PMC7166729pubmed: 19747192google scholar: lookup
        14. Grandguillot L., Fairbrother J. M., Vrins A.. Use of a protected catheter brush for culture of the lower respiratory tract in horses with small airway disease. Can. J. Vet. Res. 55: 50–55.
          pmc: PMC1263413pubmed: 1884285
        15. Hiraishi A.. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett. Appl. Microbiol. 15: 210–213.
          doi: 10.1111/j.1472-765X.1992.tb00765.xpubmed: 1280147google scholar: lookup
        16. Hiraishi A., Shin Y. K., Ueda Y., Sugiyama J.. Automated sequencing of PCR-amplified 16S rDNA on ‘Hydrolink’ gels. J. Microbiol. Methods 19: 145–154.
          doi: 10.1016/0167-7012(94)90046-9google scholar: lookup
        17. Kanoe M., Hirabayashi T., Anzai T., Imagawa H., Tanaka Y.. Isolation of obligate anaerobic and some other bacteria from equine purulent lesions. Br. Vet. J. 144: 374–378.
          doi: 10.1016/0007-1935(88)90068-1pubmed: 3167552google scholar: lookup
        18. Mair T. S., Yeo S. P.. Equine pleuropneumonia: the importance of anaerobic bacteria and the potential value of metronidazole in treatment. Vet. Rec. 121: 109–110.
          doi: 10.1136/vr.121.5.109pubmed: 3660538google scholar: lookup
        19. Park J. Y., Fox L. K., Seo K. S., McGuire M. A., Park Y. H., Rurangirwa F. R., Sischo W. M., Bohach G. A.. Comparison of phenotypic and genotypic methods for the species identification of coagulase-negative staphylococcal isolates from bovine intramammary infections. Vet. Microbiol. 147: 142–148.
          doi: 10.1016/j.vetmic.2010.06.020pmc: PMC3689435pubmed: 20667671google scholar: lookup
        20. Racklyeft D. J., Love D. N.. Bacterial infection of the lower respiratory tract in 34 horses. Aust. Vet. J. 78: 549–559.
          doi: 10.1111/j.1751-0813.2000.tb11901.xpubmed: 10979512google scholar: lookup
        21. Roberts S. A., Shore K. P., Paviour S. D., Holland D., Morris A. J.. Antimicrobial susceptibility of anaerobic bacteria in New Zealand: 1999–2003. J. Antimicrob. Chemother. 57: 992–998.
          doi: 10.1093/jac/dkl052pubmed: 16507560google scholar: lookup
        22. Seifert H., Dalhoff A.. German multicentre survey of the antibiotic susceptibility of Bacteroides fragilis group and Prevotella species isolated from intra-abdominal infections: results from the PRISMA study. J. Antimicrob. Chemother. 65: 2405–2410.
          doi: 10.1093/jac/dkq321pubmed: 20851813google scholar: lookup
        23. Sweeney C. R., Divers T. J., Benson C. E.. Anaerobic bacteria in 21 horses with pleuropneumonia. J. Am. Vet. Med. Assoc. 187: 721–724.
          pubmed: 4055490
        24. Sweeney C. R., Holcombe S. J., Barningham S. C., Beech J.. Aerobic and anaerobic bacterial isolates from horses with pneumonia or pleuropneumonia and antimicrobial susceptibility patterns of the aerobes. J. Am. Vet. Med. Assoc. 198: 839–842.
          pubmed: 2026535
        25. Sweeney R. W., Sweeney C. R., Weiher J.. Clinical use of metronidazole in horses: 200 cases (1984–1989). J. Am. Vet. Med. Assoc. 198: 1045–1048.
          pubmed: 2032913
        26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739.
          doi: 10.1093/molbev/msr121pmc: PMC3203626pubmed: 21546353google scholar: lookup

        Citations

        This article has been cited 3 times.
        1. Sun J, Xu X, Gao S, Pan Q, Liu Z, Huang Y, Lian Y. Refractory pneumonia caused by Prevotella heparinolytica: a case report. J Med Case Rep 2024 Apr 30;18(1):213.
          doi: 10.1186/s13256-024-04538-8pubmed: 38685076google scholar: lookup
        2. Lu S, Li Z, Chen X, Chen F, Yao H, Sun X, Cheng Y, Wang L, Dai P. Vaginal microbiota molecular profiling and diagnostic performance of artificial intelligence-assisted multiplex PCR testing in women with bacterial vaginosis: a single-center experience. Front Cell Infect Microbiol 2024;14:1377225.
          doi: 10.3389/fcimb.2024.1377225pubmed: 38644962google scholar: lookup
        3. Wang S, Huang A, Gu Y, Li J, Huang L, Wang X, Tao Y, Liu Z, Wu C, Yuan Z, Hao H. Rational Use of Danofloxacin for Treatment of Mycoplasma gallisepticum in Chickens Based on the Clinical Breakpoint and Lung Microbiota Shift. Antibiotics (Basel) 2022 Mar 17;11(3).
          doi: 10.3390/antibiotics11030403pubmed: 35326865google scholar: lookup
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