Donkey and horse alpha 1 B-glycoprotein: partial characterization and new alleles.
Abstract: 1. The donkey postalbumin protein has been shown to be the equivalent of human alpha 1 B-glycoprotein by protein immunoblotting and N-terminal amino acid sequence. 2. The horse A1B system (already identified as the homologue of human alpha 1 B-glycoprotein) and the donkey alpha 1 B-glycoprotein were characterized further for terminal sialic acid content, isoelectric point, amino acid composition and affinity for the dye-ligand, Cibacron Blue F3GA (known to bind human alpha 1 B-glycoprotein). 3. Two new alleles in the horse A1B system were found, bringing the total number of alleles to five. No polymorphism was found in the donkey alpha 1 B system. 4. As expected the first 20 N-terminal residues of the donkey and horse proteins are highly conserved with only two differences being found. 5. The polymorphism of the horse alpha 1 B-glycoproteins may be due in part to differing numbers of terminal sialic acid residues and the higher electrophoretic mobility of the donkey alpha 1 B-glycoprotein may be due in part to increased sialylation. 6. The horse and donkey alpha 1 B-glycoproteins exhibited differences in affinity for the dye-ligand, Cibacron Blue F3GA, with the donkey alpha 1 B-glycoprotein not being bound.
Publication Date: 1991-01-01 PubMed ID: 1868686DOI: 10.1016/0305-0491(91)90247-bGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article revolves around the study of alpha 1 B-glycoprotein in donkeys and horses. The study further examines the characteristics of this particular protein including the terminal sialic acid content, isoelectric point, amino acid composition, and its affinity towards a specific dye. The research also discovered two additional alleles in the horse, expanding the total known to five.
Identification of alpha 1 B-Glycoprotein in Donkeys
- The study confirmed the presence of alpha 1 B-glycoprotein in donkeys, which was previously known to exist in humans. This finding was achieved through protein immunoblotting and N-terminal amino acid sequence.
Characterization of Horse and Donkey alpha 1 B-Glycoproteins
- The researchers further characterized the horse A1B system and donkey alpha 1 B-glycoprotein. They investigated several characteristics including the terminal sialic acid content, isoelectric point, amino acid composition, and the affinity of the proteins for a specific dye-ligand.
- The two proteins were found to have highly conserved N-terminal residues, confirming a strong phylogenetic relationship between the species.
New Alleles Found in Horses
- In studying the horse A1B system, researchers discovered two new alleles, bringing the total number identified to five. Nonetheless, they found no such polymorphism in the donkey’s alpha 1 B system.
- The researchers speculate that the polymorphism observed in horse alpha 1 B-glycoproteins may be related to differing numbers of terminal sialic acid residues.
Variations in Affinity for Dye-Ligand
- Both the horse and donkey’ alpha 1 B-glycoproteins showed differences in their affinity for the dye-ligand, Cibacron Blue F3GA. The donkey’s alpha 1 B-glycoprotein did not bind at all to the dye.
- The research also suggested that the greater electrophoretic mobility of the donkey’s alpha 1 B-glycoprotein could, in part, be attributed to increased sialylation.
Cite This Article
APA
Patterson SD, Bell K, Shaw DC.
(1991).
Donkey and horse alpha 1 B-glycoprotein: partial characterization and new alleles.
Comp Biochem Physiol B, 98(4), 523-528.
https://doi.org/10.1016/0305-0491(91)90247-b Publication
Researcher Affiliations
- Department of Physiology and Pharmacology, University of Queensland, Australia.
MeSH Terms
- Alleles
- Amino Acid Sequence
- Amino Acids / analysis
- Animals
- Blood Proteins / chemistry
- Blood Proteins / genetics
- Electrophoresis
- Glycoproteins
- Horses / blood
- Humans
- Immunoblotting
- Immunoglobulins
- Isoelectric Point
- Molecular Sequence Data
- Neuraminidase / pharmacology
- Perissodactyla / blood
- Polymorphism, Genetic
Citations
This article has been cited 1 times.- Patterson SD, Bell K, Shaw DC. The equine major plasma serpin multigene family: partial characterization including sequence of the reactive-site regions. Biochem Genet 1991 Oct;29(9-10):477-99.
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