Doppler ultrasonography and single-fiber laser Doppler flowmetry for measurement of hind limb blood flow in anesthetized horses.
Abstract: To use Doppler ultrasonography and single-fiber laser Doppler flowmetry (LDF) to evaluate blood flow in the dependent and nondependent hind limbs of anesthetized horses and to evaluate changes in femoral arterial blood flow and microvascular skeletal muscle perfusion in response to administration of phenylephrine hydrochloride or dobutamine hydrochloride. Methods: 6 healthy adult horses. Methods: Horses were anesthetized and positioned in left lateral recumbency. Doppler ultrasonography was used to measure velocity and volumetric flow in the femoral vessels. Single-fiber LDF was used to measure relative microvascular perfusion at a single site in the semimembranosus muscles. Phenylephrine or dobutamine was then administered to decrease or increase femoral arterial blood flow, and changes in blood flow and microvascular perfusion were recorded. Results: Administration of phenylephrine resulted in significant decreases in femoral arterial and venous blood flows and cardiac output and significant increases in mean aortic blood pressure, systemic vascular resistance, and PCV. Administration of dobutamine resulted in significant increases in femoral arterial blood flow, mean aortic blood pressure, and PCV. Significant changes in microvascular perfusion were not detected. Conclusions: Results suggest that Doppler ultrasonography and single-fiber LDF can be used to study blood flows in the hind limbs of anesthetized horses. However, further studies are required to determine why changes in femoral arterial blood flows were not associated with changes in microvascular perfusion.
Publication Date: 2000-03-14 PubMed ID: 10714520DOI: 10.2460/ajvr.2000.61.286Google Scholar: Lookup
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- Journal Article
Summary
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The research article discusses an experiment that used Doppler ultrasonography and single-fiber laser Doppler flowmetry (LDF) to measure the impact of phenylephrine and dobutamine on blood flow in the hind limbs of anesthetized horses.
Introduction
- The research involved experimenting with six healthy adult horses that were anesthetized and then placed in left lateral recumbency.
- Two types of procedures were used to evaluate the blood flow – Doppler ultrasonography to determine the velocity and volumetric flow in the femoral vessels, and single-fiber LDF to gauge the relative microvascular perfusion within the semimembranosus muscles (a part of the hind limb).
Administration of Drugs
- Two types of drugs were administered to the horses – phenylephrine hydrochloride, which is known to decrease femoral arterial blood flow and dobutamine hydrochloride, which tends to increase the same.
- The impact of these drugs on blood flow and microvascular perfusion were closely recorded and monitored during the course of the experiment.
Results
- The results showed clear evidence of the impact of these drugs. The administration of phenylephrine resulted in decreased femoral arterial and venous blood flows, cardiac output, along with increased mean aortic blood pressure, systemic vascular resistance, and PCV (Packed Cell Volume).
- On the other hand, dobutamine administration led to significant increases in femoral arterial blood flow, mean aortic blood pressure, and PCV.
- Despite these changes, no significant changes were observed in microvascular perfusion – a factor that left the researchers intrigued.
Conclusion
- The research concluded that Doppler ultrasonography and single-fiber LDF could be effectively used to understand and study the blood flow in the hind limbs of anesthetized horses.
- Nevertheless, the study has left an unanswered question about the relationship between the changes in femoral arterial blood flows and microvascular perfusion, indicating the necessity of further research.
Cite This Article
APA
Raisis AL, Young LE, Taylor PM, Walsh KP, Lekeux P.
(2000).
Doppler ultrasonography and single-fiber laser Doppler flowmetry for measurement of hind limb blood flow in anesthetized horses.
Am J Vet Res, 61(3), 286-290.
https://doi.org/10.2460/ajvr.2000.61.286 Publication
Researcher Affiliations
- Center for Equine Studies, The Animal Health Trust, Newmarket, Suffolk, UK.
MeSH Terms
- Anesthetics, Dissociative
- Animals
- Arteries / diagnostic imaging
- Arteries / drug effects
- Arteries / physiology
- Blood Flow Velocity / physiology
- Blood Flow Velocity / veterinary
- Blood Pressure
- Cardiotonic Agents / pharmacology
- Dobutamine / pharmacology
- Female
- Hematocrit / veterinary
- Hindlimb / blood supply
- Hindlimb / diagnostic imaging
- Horses / physiology
- Ketamine
- Laser-Doppler Flowmetry / veterinary
- Male
- Phenylephrine / pharmacology
- Random Allocation
- Ultrasonography, Doppler / veterinary
- Vasoconstrictor Agents / pharmacology
- Videotape Recording
Citations
This article has been cited 3 times.- Tucker L, Almeida D, Wendt-Hornickle E, Baldo CF, Allweiler S, Guedes AGP. Effect of 15° Reverse Trendelenburg Position on Arterial Oxygen Tension during Isoflurane Anesthesia in Horses.. Animals (Basel) 2022 Feb 1;12(3).
- Saied GM, Kamel RM, Labib AM, Said MT, Mohamed AZ. The diabetic foot and leg: combined He-Ne and infrared low-intensity lasers improve skin blood perfusion and prevent potential complications. A prospective study on 30 Egyptian patients.. Lasers Med Sci 2011 Sep;26(5):627-32.
- Bergh A, Nyman G, Lundeberg T, Drevemo S. Effect of defocused CO2 laser on equine tissue perfusion.. Acta Vet Scand 2006;47(1):33-42.
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