Duddingtonia flagrans in the control of gastrointestinal nematodes of horses.
Abstract: Horses can harbor a large amount of parasites that may cause serious clinical signs even death. The aim of this study was to evaluate the predatory activity of the fungus Duddingtonia flagrans against infective larvae (L3) of gastrointestinal nematodes of horses in fecal culture. The experimental design was completely randomized with three treated groups (G1, G2 and G3) and one control (CG), using eight animals/group. The treated animals received G1: 1.5 × 10(5); G2: 3 × 10(5) and G3: 6 × 10(5) chlamydospores of D. flagrans/kg body weight during 21 days. The fungi preparation was given at every other three-day interval. Faecal samples were collected during 30 days, on the same interval, to perform the fecal egg counts (EPG) and fecal culture for each horse. All groups demonstrated similar results for the EPG (P > 0.05) counts. D. flagrans significantly reduced (P < 0.05) the number of infective larvae after 72 h-interval between treatments. The G2 and G3 promoted higher results (P < 0.05) of L3 reduction compared to the CG. The biological control with the predacious fungi D. flagrans is still a promising free-living parasite regulator alternative to be use in livestock.
Copyright © 2015 Elsevier Inc. All rights reserved.
Publication Date: 2015-07-22 PubMed ID: 26208781DOI: 10.1016/j.exppara.2015.07.006Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
Summary
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The research article explores the use of the fungus Duddingtonia flagrans as a method to control the number of harmful gastrointestinal nematodes in horses, highlighting a significant reduction of these parasites when the fungus is administrated at specific doses.
Research Objective
- The main goal of the study was to determine the effectiveness of the fungus Duddingtonia flagrans in reducing the quantity of infective larvae (L3) of gastrointestinal nematodes in horses.
Methodology
- The researchers used a completely randomized experimental design involving three treated groups (G1, G2, and G3) and a control group (CG). Each group included eight horses.
- The horses in the treated groups received different numbers of D. flagrans chlamydospores based on their body weight for a duration of 21 days. G1 received 1.5×10(5), G2 received 3×10(5), and G3 received 6×10(5) chlamydospores per kilogram of body weight.
- The treatment was given every three days. Similarly, fecal samples from the horses were also collected every three days for 30 days. These samples were used to measure the fecal egg counts (EPG) and perform a fecal culture.
Findings
- The results showed that there was no significant difference in the EPG counts among all groups of horses, indicating that the treatment hadn’t significantly altered the overall number of eggs produced by the parasites.
- However, the presence of D. flagrans noticeably reduced (P < 0.05) the number of infective larvae after a 72-hour interval between treatments. This means that the fungus effectively decreased the population of parasites that could potentially infect the horses.
- Moreover, groups G2 and G3, which received higher dosages of D. flagrans chlamydospores, showed a significantly higher (P < 0.05) reduction of L3 larvae compared to the control group.
- This suggests that the application of D. flagrans fungus could be further explored as a potential biological control method to regulate free-living parasites in livestock.
Cite This Article
APA
Buzatti A, de Paula Santos C, Fernandes MA, Yoshitani UY, Sprenger LK, dos Santos CD, Molento MB.
(2015).
Duddingtonia flagrans in the control of gastrointestinal nematodes of horses.
Exp Parasitol, 159, 1-4.
https://doi.org/10.1016/j.exppara.2015.07.006 Publication
Researcher Affiliations
- Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil. Electronic address: deiabuzatti@gmail.com.
- State University of North Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
- Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
- Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
- Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
- Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
- Laboratory of Parasitic Diseases, Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil; National Institute of Science and Technology - Livestock, Belo Horizonte, Brazil.
MeSH Terms
- Animals
- Duddingtonia / physiology
- Feces / parasitology
- Female
- Horse Diseases / parasitology
- Horse Diseases / therapy
- Horses
- Intestinal Diseases, Parasitic / parasitology
- Intestinal Diseases, Parasitic / therapy
- Intestinal Diseases, Parasitic / veterinary
- Larva / physiology
- Male
- Nematode Infections / parasitology
- Nematode Infections / therapy
- Nematode Infections / veterinary
- Parasite Egg Count / veterinary
- Pest Control, Biological / methods
- Time Factors
Citations
This article has been cited 9 times.- Yoon KH, Indong RA, Lee JI. Making "Sense" of Ecology from a Genetic Perspective: Caenorhabditis elegans, Microbes and Behavior.. Metabolites 2022 Nov 9;12(11).
- Li S, Wang D, Gong J, Zhang Y. Individual and Combined Application of Nematophagous Fungi as Biological Control Agents against Gastrointestinal Nematodes in Domestic Animals.. Pathogens 2022 Jan 27;11(2).
- Wernet N, Wernet V, Fischer R. The small-secreted cysteine-rich protein CyrA is a virulence factor participating in the attack of Caenorhabditis elegans by Duddingtonia flagrans.. PLoS Pathog 2021 Nov;17(11):e1010028.
- Voinot M, Bonilla R, Sousa S, Sanchís J, Canhão-Dias M, Romero Delgado J, Lozano J, Sánchez-Andrade R, Sol Arias M, Madeira de Carvalho L. Control of Strongyles in First-Season Grazing Ewe Lambs by Integrating Deworming and Thrice-Weekly Administration of Parasiticidal Fungal Spores.. Pathogens 2021 Oct 17;10(10).
- Freitas DF, da Rocha IM, Vieira-da-Motta O, de Paula Santos C. The Role of Melanin in the Biology and Ecology of Nematophagous Fungi.. J Chem Ecol 2021 Jul;47(7):597-613.
- Freitas DF, Vieira-Da-Motta O, Mathias LDS, Franco RWA, Gomes RDS, Vieira RAM, Rocha LOD, Olivares FL, Santos CP. Synthesis and role of melanin for tolerating in vitro rumen digestion in Duddingtonia flagrans, a nematode-trapping fungus.. Mycology 2019;10(4):229-242.
- Barbosa ACMS, Silva LPC, Ferraz CM, Tobias FL, de Araújo JV, Loureiro B, Braga GMAM, Veloso FBR, Soares FEF, Fronza M, Braga FR. Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans.. Int J Nanomedicine 2019;14:2341-2348.
- Hernández JÁ, Sánchez-Andrade R, Cazapal-Monteiro CF, Arroyo FL, Sanchís JM, Paz-Silva A, Arias MS. A combined effort to avoid strongyle infection in horses in an oceanic climate region: rotational grazing and parasiticidal fungi.. Parasit Vectors 2018 Apr 12;11(1):240.
- Zarrin M, Rahdar M, Poormohamadi F, Rezaei-Matehkolaei A. In Vitro Nematophagous Activity of Predatory Fungi on Infective Nematodes Larval Stage of Strongyloidae Family.. Open Access Maced J Med Sci 2017 Jun 15;5(3):281-284.
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