During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa.
Abstract: To investigate the role of the processed autophagy marker light chain 3 (LC3B) protein in sperm survival in stallion semen processing during cooled storage, split ejaculates were diluted in two different extenders, KMT and INRA 96, and LC3B processing and sperm quality evaluated during incubation at 5°C for five days. After 3 days of incubation there was a drop in total motility in both extenders, although the percentage of progressive motile sperm was greater (P<0.05) in samples extended in INRA96. On Day 5 of cooled storage all sperm parameters decreased significantly independent of the extender, however, samples extended in INRA 96 maintained motility values while those extended in KMT had a further decrease in motility compared with data collected on Day 3 of incubation. The percentage of live sperm decreased over the time of incubation, but only in samples incubated in KMT. The extender had a marked effect in LC3B processing during cooled storage. Spermatozoa maintained in KMT extender did not exhibit LC3B processing, while in spermatozoa incubated in INRA96 there was an increase (P<0.01) in LC3B processing after 5 days of cooled storage. Stallion spermatozoa experience LC3B turnover during cooled storage, however, the extent depends on the extender used. Apparently LC3B turnover is associated with enhanced survival.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication Date: 2014-01-10 PubMed ID: 24461632DOI: 10.1016/j.anireprosci.2014.01.001Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This article analyzes the influence of the extender substance used in cooled storage on the processed autophagy marker light chain 3 (LC3B) of stallion sperm. It was found that the type of extender affected the processing and quality of sperm during 5 days incubation at 5°C, with those maintained in INRA96 extender showing increased processing and better survival.
Research Methodology
- The study focused on examining the role of light chain 3 (LC3B) protein, a processed autophagy marker, in the survival of sperm during cooled storage in stallion semen processing.
- To conduct this investigation, split ejaculates were diluted in two different extenders: KMT and INRA 96.
- The processing of LC3B and the quality of sperm were evaluated during a five-day incubation period at 5°C.
Findings and Observations
- After three days of incubation, a drop in total motility was observed in both extenders. However, the percentage of progressively motile sperm was noticeably higher in samples extended in INRA96.
- By Day 5 of cooled storage, all sperm parameters had significantly decreased, regardless of the extender used.
- Samples extended in INRA 96 were able to maintain motility values, whilst those extended in KMT saw a further decrease in motility compared to data from Day 3 of incubation.
- The percentage of live sperm also decreased over the incubation period, but this was only observed in samples incubated in KMT.
Role of the Extenders
- The extender used played a significant role in LC3B processing during cooled storage.
- Spermatozoa maintained in KMT extender did not exhibit any LC3B processing, while those incubated in INRA96 showed an increase in LC3B processing after five days of cooled storage.
- The extent of LC3B turnover in the stallion spermatozoa during cooled storage depended on the type of extender used.
Conclusions
- LC3B turnover appears to be linked with enhanced survival, suggesting that the choice of extender can directly influence the survival rate of sperm during cooled storage.
Cite This Article
APA
Bolaños JM, Morán AM, da Silva CM, Dávila MP, Muñoz PM, Aparicio IM, Tapia JA, Ferrusola CO, Peña FJ.
(2014).
During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa.
Anim Reprod Sci, 145(1-2), 40-46.
https://doi.org/10.1016/j.anireprosci.2014.01.001 Publication
Researcher Affiliations
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
- Department of Physiology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
- Department of Physiology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Cáceres, Spain. Electronic address: fjuanpvega@unex.es.
MeSH Terms
- Animals
- Autophagy
- Biomarkers / metabolism
- Cell Survival / drug effects
- Cold Temperature
- Horses / metabolism
- Male
- Microtubule-Associated Proteins / metabolism
- Organ Preservation Solutions / pharmacology
- Protein Processing, Post-Translational / drug effects
- Semen Analysis / veterinary
- Semen Preservation / methods
- Semen Preservation / veterinary
- Spermatozoa / drug effects
- Spermatozoa / metabolism
Citations
This article has been cited 13 times.- Kirat D, Alahwany AM, Arisha AH, Abdelkhalek A, Miyasho T. Role of Macroautophagy in Mammalian Male Reproductive Physiology. Cells 2023 May 5;12(9).
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- Zhang Y, Xu X, Hu M, Wang X, Cheng H, Zhou R. SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy. Cell Death Differ 2021 Mar;28(3):1076-1090.
- Tesseraud S, Avril P, Bonnet M, Bonnieu A, Cassar-Malek I, Chabi B, Dessauge F, Gabillard JC, Perruchot MH, Seiliez I. Autophagy in farm animals: current knowledge and future challenges. Autophagy 2021 Aug;17(8):1809-1827.
- Shao G, Zhao Z, Zhao W, Hu G, Zhang L, Li W, Xing C, Zhang X. Long non-coding RNA MALAT1 activates autophagy and promotes cell proliferation by downregulating microRNA-204 expression in gastric cancer. Oncol Lett 2020 Jan;19(1):805-812.
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- Aparicio IM, Espino J, Bejarano I, Gallardo-Soler A, Campo ML, Salido GM, Pariente JA, Peña FJ, Tapia JA. Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility. Sci Rep 2016 Sep 16;6:33647.
- Liu T, Yang P, Chen H, Huang Y, Liu Y, Waqas Y, Ahmed N, Chu X, Chen Q. Global analysis of differential gene expression related to long-term sperm storage in oviduct of Chinese Soft-Shelled Turtle Pelodiscus sinensis. Sci Rep 2016 Sep 15;6:33296.
- Piekarski A, Khaldi S, Greene E, Lassiter K, Mason JG, Anthony N, Bottje W, Dridi S. Tissue distribution, gender- and genotype-dependent expression of autophagy-related genes in avian species. PLoS One 2014;9(11):e112449.
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- Liu T, Niu J, Huang Y, Chen H, Wu Y, Xu Y. Ultrastructural evidence for the activation of autophagy and analysis of the protective role of autophagy in goat spermatozoa under liquid storage. Front Vet Sci 2025;12:1543459.
- Raee P, Tan SC, Najafi S, Zandsalimi F, Low TY, Aghamiri S, Fazeli E, Aghapour M, Mofarahe ZS, Heidari MH, Fathabadi FF, Abdi F, Asouri M, Ahmadi AA, Ghanbarian H. Autophagy, a critical element in the aging male reproductive disorders and prostate cancer: a therapeutic point of view. Reprod Biol Endocrinol 2023 Sep 26;21(1):88.
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