Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals.
Abstract: Over many decades assisted reproductive technologies, including artificial insemination, embryo transfer, in vitro production (IVP) of embryos, cloning by somatic cell nuclear transfer (SCNT), and stem cell culture, have been developed with the aim of refining breeding strategies for improved production and health in animal husbandry. More recently, biomedical applications of these technologies, in particular, SCNT and stem cell culture, have been pursued in domestic mammals in order to create models for human disease and therapy. The following review focuses on presenting important aspects of pre-implantation development in cattle, pigs, horses, and dogs. Biological aspects and impact of assisted reproductive technologies including IVP, SCNT, and culture of pluripotent stem cells are also addressed.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication Date: 2013-06-27 PubMed ID: 23810186DOI: 10.1016/j.tvjl.2013.05.026Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Review
- Animal Models
- Animal Science
- Animal Studies
- Assisted Reproductive Techniques
- Biotechnology
- Cloning
- Developmental Biology
- Domestic Animals
- Embryo Transfer
- Equine Diseases
- Equine Medicine
- Equine model
- Equine Research
- Equine Science
- Equine Studies
- Reproductive Technology
- Stem Cells
- Veterinary Medicine
- Veterinary Research
- Veterinary Science
Summary
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This research paper focuses on the exploration and application of assisted reproductive technologies, such as artificial insemination, embryo transfer, and stem cell culture, in the field of domestic animal breeding and biomedical applications. The researchers also delve into the impact and biological aspects of these technologies.
Overview of Early Embryonic Development in Domestic Mammals
This aspect of the research draws attention to the pre-implantation development stages in domestic animals, notably cattle, pigs, horses, and dogs. Pre-implantation development is a critical aspect of reproductive biology and the researchers place emphasis on its importance.
- The paper describes the early developmental stages, from the fertilized egg to the development of the embryo.
- It gives insight into the unique traits of pre-implantation development in the featured animals.
Assisted Reproductive Technologies in Animal Husbandry
The paper provides a rich detail of the assisted reproductive technologies that have been developed over the years. The focus is more on how these technologies have been employed to refine breeding strategies for improved production and health in animal husbandry.
- The research breaks down the various assisted reproductive technologies such as artificial insemination, embryo transfer, in vitro production (IVP) of embryos, and cloning by somatic cell nuclear transfer (SCNT).
- These technologies are explained in context of their application on the featured domestic animals – cattle, pigs, horses, and dogs.
Biomedical Applications of Assisted Reproductive Technologies
The researchers also explore the biomedical applications of these technologies. Stem cell culture and SCNT are particularly emphasized in the creation of models for human disease and therapy.
- The paper looks at how these technologies are being used in the biomedical field, particularly in relation to creating replicable models for studying human diseases.
- Here, the researchers project stem cell culture and SCNT as tools with immense potential to transform the biomedical field.
Impact and biological Aspects of Assisted Reproductive Technologies
Finally, the paper digs into the overall impact and the biological aspects of these reproductive technologies. It provides a critical review of its application and the possible obstacles faced.
- It reviews the biological implications of using these technologies, noting any challenges, potential risks or ethical concerns that may arise.
- Considerations on how best to optimize the use of these technologies for maximum results are also discussed.
In conclusion, the research paper presents a holistic insight into the use of assisted reproductive technologies in the field of domestic animal reproduction and how they can be applied in the biomedical field.
Cite This Article
APA
Hall V, Hinrichs K, Lazzari G, Betts DH, Hyttel P.
(2013).
Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals.
Vet J, 197(2), 128-142.
https://doi.org/10.1016/j.tvjl.2013.05.026 Publication
Researcher Affiliations
- Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Denmark.
MeSH Terms
- Animals
- Embryo Culture Techniques / veterinary
- Embryo, Mammalian / physiology
- Embryonic Development
- Pluripotent Stem Cells / physiology
- Reproductive Techniques, Assisted / veterinary
Citations
This article has been cited 16 times.- Choi JH, Park HJ, Yang SG, Koo DB. Paclitaxel-mediated Cytoskeletal Stabilization Improves Blastocyst Developmental Competence of Bovine Embryos in Vitro. Reprod Sci 2026 Feb 12;.
- Ardiyanto D, Wuryastuty H, Yanuartono Y, Sarmin S, Wasito R, Airin CM, Widiati DT, Mubarokah WW. Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. Open Vet J 2025 Nov;15(10):5658-5668.
- de Castro RCF, Buranello TW, Recchia K, de Souza AF, Pieri NCG, Bressan FF. Emerging Contributions of Pluripotent Stem Cells to Reproductive Technologies in Veterinary Medicine. J Dev Biol 2024 May 7;12(2).
- Tsukamoto M, Kimura K, Yoshida T, Tanaka M, Kuwamura M, Ayabe T, Ishihara G, Watanabe K, Okada M, Iijima M, Nakanishi M, Akutsu H, Sugiura K, Hatoya S. Generation of canine induced pluripotent stem cells under feeder-free conditions using Sendai virus vector encoding six canine reprogramming factors. Stem Cell Reports 2024 Jan 9;19(1):141-157.
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- Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G. Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses. Stem Cells Cloning 2018;11:13-22.
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- Betts DH, Bain NT, Madan P. The p66(Shc) adaptor protein controls oxidative stress response in early bovine embryos. PLoS One 2014;9(1):e86978.
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