Effect of alpha-tocopherol and tocopherol succinate on lipid peroxidation in equine spermatozoa.
Abstract: The objective of this study was to compare the effect of alpha-tocopherol and its ester, alpha tocopherol succinate, on lipid peroxidation and motility of equine spermatozoa. In experiment one, spermatozoa were incubated with dl-alpha-tocopherol (5, 25, 100 or 500 microM), DL-alpha tocopherol succinate (5, 25, 100 or 500 microM) or vehicle (0.5% ethanol) at 38 degrees C, and sperm motility was determined at 30, 60 and 120 min. In experiment two, spermatozoa loaded with the lipophilic probe, C11BODIPY(581/591), were incubated with dl-alpha-tocopherol (50 and 100 microM), DL-alpha-tocopherol succinate (50 and 100 microM) or ethanol (0.5%) and with the promoters cumene hydroperoxide, Fe2SO4, and ascorbate at 38 degrees C in 5% CO2. Lipid peroxidation was determined by changes in fluorescence of C11BODIPY(581/591), and motility was determined by CASA at 0, 15, 30 and 60 min. In experiment three, spermatozoa loaded with C11BODIPY(581/591) were incubated with dl-alpha-tocopherol (5, 25, 100 or 500 microM), DL-alpha-tocopherol succinate (5, 25, 100 or 500 microM) or ethanol (0.5%) at 38 degrees C and then submitted to a 4-hour incubation at room temperature. Motility and lipid peroxidation were determined at 1 and 4 h. In experiment four, the effect of DL alpha tocopherol (5, 25 or 500 microM), DL-alpha-tocopherol succinate (5, 25 or 500 microM) or ethanol (0.5%) on lipid peroxidation and motility were evaluated during storage at 5 degrees C in a skim-milk based extender. Although dl-alpha-tocopherol succinate appeared more effective than DL-alpha-tocopherol in preventing lipid peroxidation during short-term incubations, the succinate ester suppressed sperm motility compared to dl-alpha-tocopherol alone.
Publication Date: 2005-05-25 PubMed ID: 15911181DOI: 10.1016/j.anireprosci.2004.12.004Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The study investigates the effects of two antioxidants (alpha-tocopherol and alpha tocopherol succinate) on lipid peroxidation and the motility of horse sperm cells. While the ester alpha tocopherol succinate showed better protection against lipid peroxidation during brief exposures, it adversely affected the movement of the sperm cells when compared to alpha-tocopherol.
Study Design
- This study involved four interrelated experiments to evaluate the effects of the antioxidants alpha-tocopherol and alpha tocopherol succinate on equine spermatozoa.
- In each experiment, varying concentrations of the antioxidants or a control substance (ethanol) were added to the sperm samples and subjected to specific conditions. Motility and lipid peroxidation, an oxidative degradation of lipids, were then measured at predefined time points.
Experiment One
- The first experiment focused on determining the impact of the antioxidants on sperm motility within a 2-hour incubation at 38 degrees Celsius.
Experiment Two
- In the second experiment, the spermatozoa were loaded with a lipophilic probe to aid in the detection of lipid peroxidation.
- They were then exposed to the antioxidants or ethanol, as well as promoters of lipid peroxidation (cumene hydroperoxide, Fe2SO4, and ascorbate) for up to an hour at the same temperature in the presence of CO2.
Experiment Three
- The third experiment subjected the sperm loaded with a lipophilic probe to a longer incubation period at room temperature after initial exposure to the antioxidants or ethanol at 38 degrees Celsius.
Experiment Four
- The final experiment sought to evaluate the antioxidants’ impact on the cells during storage at low temperature (5 degrees C) in a skim milk-based medium.
Key Findings
- The results from these experiments demonstrated that while alpha tocopherol succinate seemed more efficient in preventing lipid peroxidation during short exposure periods, it hindered the sperm’s motility when compared to alpha-tocopherol.
- This highlights the influence of the antioxidants on sperm cell function and oxidative stress response, and the need to consider multiple factors when selecting protective agents for sperm preservation.
Cite This Article
APA
Almeida J, Ball BA.
(2005).
Effect of alpha-tocopherol and tocopherol succinate on lipid peroxidation in equine spermatozoa.
Anim Reprod Sci, 87(3-4), 321-337.
https://doi.org/10.1016/j.anireprosci.2004.12.004 Publication
Researcher Affiliations
- Department of Population Health and Reproduction, University of California-Davis, Davis, CA 95616, USA.
MeSH Terms
- Animals
- Antioxidants / pharmacology
- Boron Compounds / chemistry
- Dose-Response Relationship, Drug
- Fluorescent Dyes / chemistry
- Horses / physiology
- Lipid Peroxidation / drug effects
- Lipid Peroxides / metabolism
- Male
- Oxidants / metabolism
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Motility / physiology
- Spermatozoa / drug effects
- Spermatozoa / metabolism
- Tocopherols
- Vitamin E / analogs & derivatives
- Vitamin E / pharmacology
- alpha-Tocopherol / pharmacology
Citations
This article has been cited 10 times.- Cheng B, Li D, Li C, Zhuang Z, Wang P, Liu G. The Application of Biomedicine in Chemodynamic Therapy: From Material Design to Improved Strategies. Bioengineering (Basel) 2023 Aug 3;10(8).
- Bisconti M, Grosjean P, Arcolia V, Simon JF, Hennebert E. Influence of Two Widely Used Solvents, Ethanol and Dimethyl Sulfoxide, on Human Sperm Parameters. Int J Mol Sci 2022 Dec 28;24(1).
- Dong K, Lei Q, Guo R, Wu X, Zhang Y, Cui N, Shi JY, Lu T. Regulating intracellular ROS signal by a dual pH/reducing-responsive nanogels system promotes tumor cell apoptosis. Int J Nanomedicine 2019;14:5713-5728.
- Lin J, Zhao C, Liu C, Fu S, Han L, Lu X, Yang C. Redox-responsive F127-folate/F127-disulfide bond-d-α-tocopheryl polyethylene glycol 1000 succinate/P123 mixed micelles loaded with paclitaxel for the reversal of multidrug resistance in tumors. Int J Nanomedicine 2018;13:805-830.
- Berlinguer F, Pasciu V, Succu S, Cossu I, Caggiu S, Addis D, Castagna A, Fontani V, Rinaldi S, Passino ES. REAC technology as optimizer of stallion spermatozoa liquid storage. Reprod Biol Endocrinol 2017 Feb 8;15(1):11.
- Dong K, Yan Y, Wang P, Shi X, Zhang L, Wang K, Xing J, Dong Y. Biodegradable mixed MPEG-SS-2SA/TPGS micelles for triggered intracellular release of paclitaxel and reversing multidrug resistance. Int J Nanomedicine 2016;11:5109-5123.
- Azawi OI, Hussein EK. Effect of vitamins C or E supplementation to Tris diluent on the semen quality of Awassi rams preserved at 5 ˚C. Vet Res Forum 2013 Summer;4(3):157-60.
- Martorana K, Klooster K, Meyers S. Suprazero cooling rate, rather than freezing rate, determines post thaw quality of rhesus macaque sperm. Theriogenology 2014 Feb;81(3):381-8.
- Towhidi A, Parks JE. Effect of n-3 fatty acids and α-tocopherol on post-thaw parameters and fatty acid composition of bovine sperm. J Assist Reprod Genet 2012 Oct;29(10):1051-6.
- Díaz Ruiz E, Navas González FJ, León Jurado JM, Arando Arbulu A, Delgado Bermejo JV, González Ariza A. Effects of Supplementation of Different Antioxidants to Cryopreservation Extender on the Post-Thaw Quality of Rooster Semen-A Meta-Analysis. Animals (Basel) 2024 Oct 11;14(20).
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