Effect of antigen challenge on dynamics of CD62P and CD41/61 expression on platelets in horses with recurrent airway obstruction (RAO).
Abstract: Recurrent airway obstruction (RAO), also known as heaves, is an allergic respiratory condition that develops in horses following an exposure to aeroallergens in hay and straw. This is manifested by airway hyperreactivity, inflammation, bronchoconstriction, as well as a leukocyte and platelet infiltration into the airways. Platelet activation and an increase in circulating platelet-leukocyte aggregates may lead to airway remodeling. The aim of this study was to explore the effect of seven-day antigen challenge on dynamics of platelet indices and CD41/61 and CD62 P expression on platelets in horses with RAO. Ten RAO-affected horses and ten healthy horses were included in this study. All horses were exposed to 7 days hay and straw challenge. Blood samples were collected prior to the challenge (Pre-challenge) and 1, 2, 3, 7 and 14 days after the initiating the antigen challenge. Blood samples were obtained to determine the platelet count (PLT), mean platelet volume (MPV) and platelet large cell ratio (P-LCR). Expression of CD62 P and CD41/61 was detected by flow cytometry on activated platelets. Antigen challenge resulted in a significant gradual decrease of PLT in RAO horses, but not in controls. MPV and P-LCR in control and RAO-affected horses remained unchanged after antigen challenge. The expression of CD62 P and CD41/61 in RAO horses was significantly higher compared to control horses. The antigen challenge resulted in an increase expression of CD62 P and CD41/61 on the platelets of RAO-affected horses, while did not lead to significant changes in the control group. An increased expression of CD62 P and CD41/61 indicates platelet activation what may contribute to the formation of platelet aggregates in their respiratory system.
Copyright © 2018 Elsevier B.V. All rights reserved.
Publication Date: 2018-07-18 PubMed ID: 30078592DOI: 10.1016/j.vetimm.2018.07.007Google Scholar: Lookup
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Summary
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This research examines how exposure to certain allergens affects platelet behaviour in horses suffering from recurrent airway obstruction (RAO), an allergic respiratory condition. The aim was to explore changes in platelet index and specific protein expressions on platelets after a seven-day antigen challenge.
Study Background
- Recurrent airway obstruction (RAO), also known as heaves, is a common respiratory condition in horses resulting from exposure to allergens present in fodder like hay and straw.
- RAO results in a hyper-responsive reaction in the horse’s airways, encompassing inflammation and bronchoconstriction, which further leads to infiltration of leukocytes and platelets into the airways.
- This study aimed to assess the effect of antigen challenge on platelet indices including CD41/61 and CD62P expression on platelets in horses with RAO.
Research Methodology
- A total of 20 horses was used in the study. Ten horses with diagnosed RAO and ten healthy horses as controls. All horses were exposed to the same seven-day antigen challenge involving hay and straw.
- Blood samples were collected from each horse before the challenge and at defined intervals after the challenge—1, 2, 3, 7, and 14 days.
- A range of indices were evaluated from the blood samples including platelet count (PLT), mean platelet volume (MPV), and platelet large cell ratio (P-LCR).
- Flow cytometry was used to detect the expression of CD62P and CD41/61 proteins on activated platelets in the samples.
Research Findings
- In RAO-affected horses, there was a significant decrease in platelet count (PLT) following the antigen challenge, while this was not observed in the control group.
- Antigen exposure did not significantly alter the mean platelet volume (MPV) or platelet large cell ratio (P-LCR) in either set of horses.
- The study indicated significantly higher expressions of CD62P and CD41/61 proteins on platelets in RAO horses as opposed to the healthy controls.
- Post the antigen challenge, there was a marked increase in CD62P and CD41/61 expressions in RAO horses, but there was no significant change in controls.
- Increased expression of CD62P and CD41/61 suggests increased platelet activity, which may contribute to the formation of platelet aggregates in the horse’s respiratory system.
Conclusion
- This research provides insight into how antigen challenge affects platelet behaviour in RAO-affected horses. These results can contribute towards understanding the role of platelets and specific proteins in the pathogenesis of RAO in horses.
Cite This Article
APA
Iwaszko A, Borowicz H, Graczyk S, Slowikowska M, Pliszczak-Krol A, Niedzwiedz A.
(2018).
Effect of antigen challenge on dynamics of CD62P and CD41/61 expression on platelets in horses with recurrent airway obstruction (RAO).
Vet Immunol Immunopathol, 202, 172-180.
https://doi.org/10.1016/j.vetimm.2018.07.007 Publication
Researcher Affiliations
- Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: alicja.iwaszko83@gmail.com.
- Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: hieronim.borowicz@upwr.edu.pl.
- Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: stanislaw.graczyk@upwr.edu.pl.
- Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: malwina.slowikowska@upwr.edu.pl.
- Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: aleksandra.krol@upwr.edu.pl.
- Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. Electronic address: artur.niedzwiedz@upwr.edu.pl.
MeSH Terms
- Airway Obstruction / immunology
- Allergens / administration & dosage
- Animal Feed
- Animals
- Blood Platelets / cytology
- Bronchoalveolar Lavage Fluid / cytology
- Female
- Horse Diseases / immunology
- Horses / immunology
- Hypersensitivity / veterinary
- Male
- P-Selectin / metabolism
- Platelet Activation
- Platelet Membrane Glycoprotein IIb / metabolism
- Pulmonary Disease, Chronic Obstructive / immunology
- Pulmonary Disease, Chronic Obstructive / veterinary
Citations
This article has been cited 1 times.- Theuerkauf K, Obach-Schröck C, Staszyk C, Moritz A, Roscher KA. Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome. J Vet Diagn Invest 2022 May;34(3):448-457.
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