Effect of calcium-channel blockers and salbutamol on the isolated mare uterus–interaction with the calcium agonist Bay K 8644.
Abstract: The effects of nifedipine, verapamil and diltiazem were investigated in the isolated mare uterus in comparison with salbutamol. All the calcium-channel blockers and salbutamol inhibited the spontaneous, KC1- and electrically induced contractions; nifedipine and salbutamol were the most potent compounds. The calcium agonist Bay K 8644 (10(-8)-10(-6) mol/l) competitively antagonized the inhibitory effect of nifedipine (pA2 value = 8.54 +/- 0.06), whereas it was only slightly or totally ineffective against verapamil, diltiazem and salbutamol. These results indicate that calcium-channel blockers are potent inhibitors of mare uterine motility in vitro and emphasize the importance of Ca2+-related mechanisms in the control of uterine smooth-muscle contractility. Moreover, the validity of Bay K 8644 as a tool to distinguish classes of calcium-channel antagonists is confirmed.
Publication Date: 1989-12-01 PubMed ID: 2482371DOI: 10.1111/j.1365-2885.1989.tb00691.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the effects of certain drugs, particularly calcium-channel blockers, on the isolated uterus of mares. It also explores the interaction of these drugs with a calcium agonist, Bay K 8644.
Objective and Methods
- The researchers aimed at examining the effects of certain calcium-channel blockers, namely nifedipine, verapamil, and diltiazem, on mare uterine activity.
- The comparison was made using salbutamol, which is not directly a calcium-channel blocker but works on similar principles.
- The study was performed on an isolated mare uterus where the researchers recorded the impact on spontaneous, KC1- (a form of potassium solution), and electrically induced contractions.
Results and Observations
- All the calcium-channel blockers and salbutamol were found to inhibit uterine contractions.
- Out of these drugs, nifedipine and salbutamol exhibited the most potently inhibitory effects.
- Interestingly, the calcium agonist Bay K 8644, when present in the range of 10-8 to 10-6 mol/l, competitively antagonized the inhibitory effect of nifedipine. This means that Bay K 8644 blocked the inhibitory action of nifedipine on uterine contractions.
- However, Bay K 8644 showed limited or no effect against the activity of verapamil, diltiazem, and salbutamol.
Conclusions and Implications
- The research concluded that calcium-channel blockers are effective inhibitors of mare uterine motility in vitro, indicating the significance of Ca2+-related (calcium-ion related) mechanisms in the control of uterine smooth-muscle contractility.
- The study also confirmed the suitability of Bay K 8644 as a tool to distinguish among the various classes of calcium-channel antagonists.
- The study opens up inquiry into the role of specific calcium-channel blockers and agonists in controlling uterine contractions, which might have broader implications in the management of reproductive health in equines and potentially other species.
Cite This Article
APA
Coruzzi G, Poli E, Bertaccini G.
(1989).
Effect of calcium-channel blockers and salbutamol on the isolated mare uterus–interaction with the calcium agonist Bay K 8644.
J Vet Pharmacol Ther, 12(4), 404-410.
https://doi.org/10.1111/j.1365-2885.1989.tb00691.x Publication
Researcher Affiliations
- Institute of Pharmacology, Faculty of Veterinary, University of Parma, Italy.
MeSH Terms
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
- Albuterol / pharmacology
- Animals
- Calcium Channel Blockers / pharmacology
- Diltiazem / pharmacology
- Drug Interactions
- Female
- Horses / physiology
- Nifedipine / pharmacology
- Uterine Contraction / drug effects
- Uterus / drug effects
- Uterus / physiology
- Verapamil / pharmacology
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