Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter.
Abstract: K influx into equine red blood cells (RBCs) was measured using 86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension (PO2), CO2 tension (PCO2), and bicarbonate and lactate concentrations were defined. Physiological PO2 exerted a dominant controlling influence on the H(+)-stimulated Cl-dependent K influx, consistent with effects on the K-Cl cotransporter, PO2 required for half-maximal activity was 37 +/- 3 mmHg (4.9 kPa). Although RBCs were swollen at low pH, results showed explicitly that the volume change per se had little effect on K influx. Lactate had no effect on volume- or H(+)-stimulated K influxes, nor did bicarbonate or PCO2 affect the magnitude of K influxes after these stimuli or after treatment with protein kinase/phosphatase inhibitors. These results represent the first detailed report of O2 dependence of H(+)-stimulated K-Cl cotransport in RBCs from any mammalian species. They emphasize the importance of PO2 in control of RBC K-Cl cotransport.
Publication Date: 1998-01-22 PubMed ID: 9435484DOI: 10.1152/ajpcell.1997.273.6.C1811Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study conducted experiments on equine red blood cells to understand the impact of changes in respiratory blood parameters, mimicking conditions during heavy exercise, on potassium-chloride cotransporter. It demonstrates the significant role oxygen tension plays in regulating this cotransport mechanism, overriding the effects of other physiological changes normally observed during strenuous activity.
Introduction and Objective
- The research focused on the role of respiratory blood parameters during strenuous exercise, specifically their impact on potassium-chloride cotransport in equine red blood cells.
Methodology and Results
- Potassium influx into red blood cells (RBCs) of horses was tracked using 86Rb, a tracer for potassium.
- The experiment sought to replicate the physiological changes that occur inside the body during hard exercise, such as variations in pH, cell volume, oxygen tension (PO2), carbon dioxide tension (PCO2), and concentrations of bicarbonate and lactate.
- The study determined that oxygen tension exerted the most control over the H(+)-stimulated chloride-dependent potassium influx, aligning with its effects on the potassium-chloride cotransporter.
- The oxygen tension level for half-maximal activity was identified as 37 +/- 3 mmHg.
- Despite cells swelling at lower pH levels, the volume change itself had barely any impact on potassium influx.
- Similarly, lactate didn’t have any effect on the volume or H(+)-stimulated potassium influx, and neither did bicarbonate or carbon dioxide tension affect the potassium influx after the stimuli or post-treatment with protein kinase/phosphatase inhibitors.
Significance and Conclusion
- This research is the first detailed report on the oxygen dependence of H(+)-stimulated potassium-chloride cotransport in RBCs of any mammalian species.
- It underscores the pivotal role oxygen tension plays in controlling potassium-chloride cotransport in red blood cells, especially as compared to other physiological changes, substantiating the importance of maintaining adequate oxygen levels during intense physical exertion.
Cite This Article
APA
Speake PF, Roberts CA, Gibson JS.
(1998).
Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter.
Am J Physiol, 273(6), C1811-C1818.
https://doi.org/10.1152/ajpcell.1997.273.6.C1811 Publication
Researcher Affiliations
- Department of Veterinary Preclinical Sciences, University of Liverpool, United Kingdom.
MeSH Terms
- Animals
- Bicarbonates / blood
- Body Water
- Carbon Dioxide / blood
- Carrier Proteins / blood
- Erythrocytes / metabolism
- Hematocrit
- Horses / physiology
- Hydrogen-Ion Concentration
- Lactates / blood
- Oxygen / blood
- Partial Pressure
- Physical Exertion / physiology
- Potassium / blood
- Respiration
- Rubidium Radioisotopes / blood
- Symporters
Grant Funding
- Wellcome Trust
Citations
This article has been cited 21 times.- Lu DC, Hannemann A, Gibson JS. Does Plasma Inhibit the Activity of KCl Cotransport in Red Cells From LK Sheep?. Front Physiol 2022;13:904280.
- Al Balushi H, Hannemann A, Rees D, Brewin J, Gibson JS. The Effect of Antioxidants on the Properties of Red Blood Cells From Patients With Sickle Cell Anemia.. Front Physiol 2019;10:976.
- Al Balushi H, Dufu K, Rees DC, Brewin JN, Hannemann A, Oksenberg D, Lu DC, Gibson JS. The effect of the antisickling compound GBT1118 on the permeability of red blood cells from patients with sickle cell anemia.. Physiol Rep 2019 Mar;7(6):e14027.
- Frlic O, Seliškar A, Domanjko Petrič A, Blagus R, Heigenhauser G, Vengust M. Pulmonary Circulation Transvascular Fluid Fluxes Do Not Change during General Anesthesia in Dogs.. Front Physiol 2018;9:124.
- Al Balushi HWM, Rees DC, Brewin JN, Hannemann A, Gibson JS. The effect of xanthine oxidase and hypoxanthine on the permeability of red cells from patients with sickle cell anemia.. Physiol Rep 2018 Mar;6(5).
- Hannemann A, Rees DC, Tewari S, Gibson JS. Cation Homeostasis in Red Cells From Patients With Sickle Cell Disease Heterologous for HbS and HbC (HbSC Genotype).. EBioMedicine 2015 Nov;2(11):1669-76.
- Hannemann A, Cytlak UM, Rees DC, Tewari S, Gibson JS. Effects of 5-hydroxymethyl-2-furfural on the volume and membrane permeability of red blood cells from patients with sickle cell disease.. J Physiol 2014 Sep 15;592(18):4039-49.
- Hannemann A, Cytlak UM, Gbotosho OT, Rees DC, Tewari S, Gibson JS. Effects of o-vanillin on K⁺ transport of red blood cells from patients with sickle cell disease.. Blood Cells Mol Dis 2014 Jun-Aug;53(1-2):21-6.
- Gbotosho OT, Cytlak UM, Hannemann A, Rees DC, Tewari S, Gibson JS. Inhibitors of second messenger pathways and Ca(2+)-induced exposure of phosphatidylserine in red blood cells of patients with sickle cell disease.. Pflugers Arch 2014 Jul;466(7):1477-85.
- Cytlak UM, Hannemann A, Rees DC, Gibson JS. Identification of the Ca²⁺ entry pathway involved in deoxygenation-induced phosphatidylserine exposure in red blood cells from patients with sickle cell disease.. Pflugers Arch 2013 Nov;465(11):1651-60.
- Milligan C, Rees DC, Ellory JC, Osei A, Browning JA, Hannemann A, Gibson JS. A non-electrolyte haemolysis assay for diagnosis and prognosis of sickle cell disease.. J Physiol 2013 Mar 15;591(6):1463-74.
- Chu H, Breite A, Ciraolo P, Franco RS, Low PS. Characterization of the deoxyhemoglobin binding site on human erythrocyte band 3: implications for O2 regulation of erythrocyte properties.. Blood 2008 Jan 15;111(2):932-8.
- Browning JA, Staines HM, Robinson HC, Powell T, Ellory JC, Gibson JS. The effect of deoxygenation on whole-cell conductance of red blood cells from healthy individuals and patients with sickle cell disease.. Blood 2007 Mar 15;109(6):2622-9.
- Berenbrink M, Völkel S, Koldkjaer P, Heisler N, Nikinmaa M. Two different oxygen sensors regulate oxygen-sensitive K+ transport in crucian carp red blood cells.. J Physiol 2006 Aug 15;575(Pt 1):37-48.
- Muzyamba MC, Campbell EH, Gibson JS. Effect of intracellular magnesium and oxygen tension on K+-Cl- cotransport in normal and sickle human red cells.. Cell Physiol Biochem 2006;17(3-4):121-8.
- Vengust M, Staempfli H, Viel L, Heigenhauser G. Transvascular fluid flux from the pulmonary vasculature at rest and during exercise in horses.. J Physiol 2006 Jan 15;570(Pt 2):397-405.
- Adragna NC, Di Fulvio M, Lauf PK. Regulation of K-Cl cotransport: from function to genes.. J Membr Biol 2004 Oct 1;201(3):109-37.
- Dunham PB. Oxygen sensing and K(+)-Cl(-) cotransport.. J Physiol 2000 Jul 1;526 Pt 1(Pt 1):1.
- Muzyamba MC, Cossins AR, Gibson JS. Regulation of Na+-K+-2Cl- cotransport in turkey red cells: the role of oxygen tension and protein phosphorylation.. J Physiol 1999 Jun 1;517 ( Pt 2)(Pt 2):421-9.
- Campbell EH, Cossins AR, Gibson JS. Oxygen-dependent K+ influxes in Mg2+-clamped equine red blood cells.. J Physiol 1999 Mar 1;515 ( Pt 2)(Pt 2):431-7.
- Gibson JS, Speake PF, Ellory JC. Differential oxygen sensitivity of the K+-Cl- cotransporter in normal and sickle human red blood cells.. J Physiol 1998 Aug 15;511 ( Pt 1)(Pt 1):225-34.
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