Effect of cycloheximide on nuclear maturation of horse oocytes and its relation to initial cumulus morphology.
Abstract: The period of protein synthesis necessary for meiotic maturation in horse oocytes initially having compact or expanded cumulus cells was studied. Oocytes incubated in the presence of cycloheximide after 0, 4, 8, 12 or 16 h maturation in vitro (total incubation time 24 h) were matured for 24 h, or were incubated with cycloheximide for 24 h and then matured for 24 h. Incubation with cycloheximide from 0 h was effective in suppressing maturation (no significant increase in maturing oocytes compared with controls fixed directly after removal from the follicle) in both expanded and compact groups and was completely reversible, as there was no difference in the proportion of oocytes reaching metaphase II between controls and treatment groups of either cumulus type. Addition of cycloheximide after 4 h maturation resulted in no significant difference in distribution of oocytes compared with addition at 0 h in either cumulus group. A significant decrease in the proportion of germinal vesicle stage oocytes, and an increase in oocytes in metaphase I occurred in oocytes with expanded cumulus cells in the 8 h treatment and in oocytes with compact cumulus cells in the 12 h treatment, compared with oocytes treated after 0 h. A significant increase in the proportion of oocytes at metaphase II occurred in the 12 h treatment for expanded cumulus-oocyte complexes and in the 16 h treatment for compact cumulus-oocyte complexes. These data show that nuclear maturation of horse oocytes can be reversibly suppressed by incubation with cycloheximide from the onset of culture. Oocytes with different initial cumulus type differed in the time required for protein synthesis essential for maturation: expanded cumulus-oocyte complexes required less time to prepare for germinal vesicle breakdown, maturation to metaphase I, and maturation to metaphase II, than did compact cumulus-oocyte complexes.
Publication Date: 1996-07-01 PubMed ID: 8882287DOI: 10.1530/jrf.0.1070215Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study focuses on understanding the required duration of protein synthesis in horse oocytes with different initial cumulus morphology, specifically expanded or compact, for meiotic maturation. The research demonstrates that the medication cycloheximide can reversibly halt the nuclear maturation in horse oocytes and finds a difference in the time needed for protein synthesis essential for maturation between oocytes with different initial cumulus types.
Research Methodology
- The researchers incubated horse oocytes in the presence of cycloheximide after different durations of maturation in vitro (0, 4, 8, 12, or 16 hours).
- Incubation was conducted for a total time of 24 hours.
- The oocytes were either matured for 24 hours, or they were incubated with the drug for 24 hours followed by a maturation period of 24 hours.
- The outcome of maturation was compared between the treated groups and control groups.
Findings and Conclusion
- Incubation with cycloheximide from the start was effective in suppressing maturation in both expanded and compact groups.
- Suppression of maturation was completely reversible. As a result, there was no difference in the proportion of oocytes reaching metaphase II between controls and treatment groups.
- Addition of cycloheximide after 4 hours of maturation did not result in a significant difference in the distribution of oocytes compared to those treated from the start.
- Oocytes with expanded cumulus cells underwent an increase in metaphase I oocytes after 8 hours’ treatment, and those with compact cumulus cells noticed the same after the 12 hour treatment.
- Metaphase II was noticed significantly more on the 12 hour treatment for expanded cumulus-oocyte complexes and on the 16-hour treatment for compact cumulus-oocyte complexes.
- The conclusion of the study was that nuclear maturation in horse oocytes can be reversibly halted with cycloheximide, and that the time needed for protein synthesis which is essential for maturation differs based on the initial cumulus type of the oocyte.
- This suggests that expanded cumulus-oocyte complexes need less time for germinal vesicle breakdown and maturation compared to compact cumulus-oocyte complexes.
Cite This Article
APA
Alm H, Hinrichs K.
(1996).
Effect of cycloheximide on nuclear maturation of horse oocytes and its relation to initial cumulus morphology.
J Reprod Fertil, 107(2), 215-220.
https://doi.org/10.1530/jrf.0.1070215 Publication
Researcher Affiliations
- Department of Reproductive Biology, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany.
MeSH Terms
- Animals
- Cell Nucleus / drug effects
- Cells, Cultured
- Cycloheximide / pharmacology
- Female
- Horses / physiology
- Oocytes / cytology
- Oocytes / drug effects
- Oogenesis / drug effects
- Protein Synthesis Inhibitors / pharmacology
- Time Factors
Citations
This article has been cited 1 times.- Orsolini MF, Meyers SA, Dini P. An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section II. Animals (Basel) 2021 Nov 20;11(11).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
