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Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2019; 31(4); 585-587; doi: 10.1177/1040638719860877

Effect of delayed plasma centrifugation on equine adrenocorticotropic hormone concentration.

Abstract: Plasma adrenocorticotropic hormone (ACTH) concentration is used in the diagnosis of pituitary pars intermedia dysfunction (PPID) in horses. We enrolled 10 horses, 5 PPID-positive and 5 PPID-negative, in our study, September 20-22, 2016. On day 0, 5 mL of whole blood was collected into each of 6 EDTA tubes and immediately placed in a refrigerator at 7°C. One tube was centrifuged within 15 min of collection, followed by centrifugation of one tube from each horse at 4, 8, 12, 24, and 36 h following collection. At each time, centrifuged plasma was pipetted into 1.5-mL polypropylene tubes and stored at -80°C. None of the plasma samples were turbid, hemolyzed, or icteric. Plasma was shipped frozen with cold packs overnight to the Animal Health Diagnostic Center of Cornell University (Ithaca, NY) for analysis. The percent change from baseline (PCFB) was reported to standardize the data given that baseline values differed. The mean PCFB was 2.8 (95% confidence interval: -2.9%, 7.0%). Neither refrigeration of whole blood for up to 36 h prior to centrifugation nor freezing affected plasma ACTH concentrations significantly.
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Publication Date: 2019-06-27 PubMed ID: 31246158PubMed Central: PMC6857008DOI: 10.1177/1040638719860877Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Researchers have found that the timing of blood plasma centrifugation doesn’t have a significant effect on the measurement of adrenocorticotropic hormone (ACTH) levels in horses, which is important for diagnosing a condition known as pituitary pars intermedia dysfunction (PPID).

Objective and Methodology of the Study

  • This study aimed to determine the effect of delayed plasma centrifugation on the measurement of ACTH concentrations in blood. This hormone is essential for diagnosing PPID in horses.
  • 10 horses, half with PPID and half without, were enrolled in the research during a period in September 2016.
  • The research team collected blood samples from each horse, with the samples immediately put in refrigeration.
  • The blood samples were then centrifuged at different time intervals (15 minutes, 4 hours, 8 hours, 12 hours, 24 hours, and 36 hours) after the collection of the sample.

Data Collection and Analysis

  • After each centrifugation, the plasma was extracted and stored in a frozen state.
  • The samples were sent to the Animal Health Diagnostic Center of Cornell University for further analysis.
  • To normalize the data, considering the differing baseline values, the researchers used the percent change from the baseline (PCFB).
  • The average PCFB was found to be 2.8% with a 95% confidence interval between -2.9% and 7.0%.

Findings and Conclusions

  • The main finding of the research was that neither the refrigeration of whole blood for up to 36 hours before centrifugation nor freezing of the samples caused significant changes in plasma ACTH concentrations.
  • Therefore, the researchers concluded that the timing of plasma centrifugation does not critically alter the results of plasma ACTH concentration measurement in horses.

Cite This Article

APA
Shepard KN, Haffner JC, Neal DL, Grubbs ST, Pearce GL. (2019). Effect of delayed plasma centrifugation on equine adrenocorticotropic hormone concentration. J Vet Diagn Invest, 31(4), 585-587. https://doi.org/10.1177/1040638719860877

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 31
Issue: 4
Pages: 585-587

Researcher Affiliations

Shepard, Kayla N
  • Boehringer Ingelheim Vetmedica, St. Joseph, MO (Shepard, Neal, Grubbs).
  • Middle Tennessee State University, Murfreesboro, TN (Haffner).
  • Innovative Data Resources, Asheville, NC (Pearce).
Haffner, John C
  • Boehringer Ingelheim Vetmedica, St. Joseph, MO (Shepard, Neal, Grubbs).
  • Middle Tennessee State University, Murfreesboro, TN (Haffner).
  • Innovative Data Resources, Asheville, NC (Pearce).
Neal, Dwana L
  • Boehringer Ingelheim Vetmedica, St. Joseph, MO (Shepard, Neal, Grubbs).
  • Middle Tennessee State University, Murfreesboro, TN (Haffner).
  • Innovative Data Resources, Asheville, NC (Pearce).
Grubbs, Steven T
  • Boehringer Ingelheim Vetmedica, St. Joseph, MO (Shepard, Neal, Grubbs).
  • Middle Tennessee State University, Murfreesboro, TN (Haffner).
  • Innovative Data Resources, Asheville, NC (Pearce).
Pearce, Greg L
  • Boehringer Ingelheim Vetmedica, St. Joseph, MO (Shepard, Neal, Grubbs).
  • Middle Tennessee State University, Murfreesboro, TN (Haffner).
  • Innovative Data Resources, Asheville, NC (Pearce).

MeSH Terms

  • Adrenocorticotropic Hormone / blood
  • Animals
  • Centrifugation / veterinary
  • Horse Diseases / blood
  • Horse Diseases / diagnosis
  • Horses
  • Pituitary Diseases / blood
  • Pituitary Diseases / diagnosis
  • Pituitary Diseases / veterinary
  • Pituitary Gland, Intermediate / pathology

Conflict of Interest Statement

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

References

This article includes 12 references
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Citations

This article has been cited 2 times.
  1. Thane K, Uricchio C, Frank N. Effect of early or late blood sampling on thyrotropin releasing hormone stimulation test results in horses. J Vet Intern Med 2022 Mar;36(2):770-777.
    doi: 10.1111/jvim.16362pubmed: 35049089google scholar: lookup
  2. Tsuchiya T, Noda R, Ikeda H, Maeda M, Sato F. Relationship between endogenous plasma adrenocorticotropic hormone concentration and reproductive performance in Thoroughbred broodmares. J Vet Intern Med 2021 Jul;35(4):2002-2008.
    doi: 10.1111/jvim.16145pubmed: 34028083google scholar: lookup
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