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Home / Equine Research Bank / Vet Clin Pathol / Effect of dipotassium-ethylenediaminetetraacetic acid or lit...
Veterinary clinical pathology2023; doi: 10.1111/vcp.13287

Effect of dipotassium-ethylenediaminetetraacetic acid or lithium-heparin treatments and storage times on selected clinicopathologic analytes in equine synovial fluid.

Abstract: Sample processing methods and storage time affect the outcome of biochemical analysis. Objectives: We aimed to assess the effects of dipotassium-ethylenediaminetetraacetic acid (K2-EDTA) and lithium-heparin treatments and storage times on selected analytes in equine synovial fluid (SF). Conclusions: The analytes-except for TP-became unstable within a few hours postcollection. Lithium-heparin and K2-EDTA treatments significantly altered ALP, LDH, TNCCs, and pH but not the TP concentrations of equine SF. Studies establishing reference intervals for these analytes based on the anticoagulant used are warranted to limit misinterpretations in clinical or research settings.
© 2023 American Society for Veterinary Clinical Pathology.
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Publication Date: 2023-07-26 PubMed ID: 37495547DOI: 10.1111/vcp.13287Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study looked at how different treatments and storage times might change the results of tests done on horse joint fluid.

The treatments and storage times can affect test results of horse joint fluid. It’s important to consider these factors to get accurate test results, especially when using the fluid for medical or research purposes.

Background

  • Methods used for sample processing and the duration they are stored can influence the results of biochemical analyses.

Objectives

  • The goal was to study the effects of treatments using dipotassium-ethylenediaminetetraacetic acid (K2-EDTA) and lithium-heparin, as well as varying storage times, on specific analytes present in equine synovial fluid (SF).

Methods

  • 2 mL of SF was collected from each of the 7 horses through femoropatellar joint arthrocentesis.
  • The fluid was stored in bottles treated with K2-EDTA (K2-EDTA group), bottles treated with lithium-heparin (heparin group), and untreated bottles (control group).
  • An electronic bench pH meter was used to measure the pH of the samples.
  • The total nucleated cell count (TNCC) was determined using the hemocytometer method.
  • Total protein (TP) concentrations, lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) activities were measured spectrophotometrically at multiple time points (2, 8, 24, 48, and 168 hours) postcollection, with the samples kept at roughly 4°C.

Results

  • TP concentrations in the samples treated with anticoagulants were stable up to 48 hours.
  • TNCCs remained consistent for up to 8 hours.
  • Significant variations were observed in ALP, LDH, and pH values after just 2 hours (P < 0.05).
  • At the 2-hour mark, lithium-heparin treated samples showed notably increased ALP and LDH activities, while the K2-EDTA and control groups displayed similar activity levels for these analytes.
  • By 8 hours, the K2-EDTA treated samples exhibited significantly elevated TNCC and pH, but the lithium-heparin and control groups showed comparable values.
  • There were no significant changes in TP values at 2 hours across all treatment groups.

Conclusions

  • Except for TP, the analytes displayed instability within hours after collection.
  • Both lithium-heparin and K2-EDTA treatments considerably impacted the values of ALP, LDH, TNCCs, and pH, but not TP, in the equine SF samples.
  • Further studies are needed to set reference intervals for these analytes based on the specific anticoagulant used. This is essential to prevent misinterpretations in both clinical and research contexts.

Cite This Article

APA
Okolo CC, Emejuo NT, Udeagbala NG, Emeto UE, Ezema AS, Omeje OV, Nweze NE. (2023). Effect of dipotassium-ethylenediaminetetraacetic acid or lithium-heparin treatments and storage times on selected clinicopathologic analytes in equine synovial fluid. Vet Clin Pathol. https://doi.org/10.1111/vcp.13287

Publication

Veterinary clinical pathology
ISSN: 1939-165X
NlmUniqueID: 9880575
Country: United States
Language: English

Researcher Affiliations

Okolo, Chukwuemeka C
  • Department of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
Emejuo, Nnenna T
  • Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
Udeagbala, Ndidiamaka G
  • Department of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
Emeto, Uzochukwu E
  • Department of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
Ezema, Arinzechukwu S
  • Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
Omeje, Okonkwo V
  • Department of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
Nweze, Nwakaego E
  • Department of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.

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