Effect of exercise and osteochondral injury on synovial fluid and serum concentrations of carboxy-terminal telopeptide fragments of type II collagen in racehorses.
Abstract: To investigate the effects of exercise and osteochondral injury on concentrations of carboxy-terminal telopeptide fragments of type II collagen (CTX-II) in synovial fluid (SF) and serum of Thoroughbred racehorses and to compare findings with radiographic and arthroscopic scores of joint injury severity. Methods: 78 Thoroughbreds with (n = 38) and without (40) osteochondral injury. Methods: Serum and metacarpophalangeal or carpal joint SF samples were collected from noninjured horses before and at the end of 5 to 6 months of race training (pre- and postexercise samples, respectively) and from horses with osteochondral injury (1 joint assessed/horse). Synovial fluid and serum CTX-II concentrations were determined by use of an ELISA. Radiographic and arthroscopic scores of joint injury severity were determined for the injured horses. Results: The CTX-II concentrations in SF and SF:serum CTX-II ratio were significantly higher for horses with joint injuries, compared with pre- and postexercise findings in noninjured horses. Serum CTX-II concentrations in postexercise and injured-horse samples were significantly lower than values in pre-exercise samples. On the basis of serum and SF CTX-II concentrations and SF:serum CTX-II ratio, 64% to 93% of serum and SF samples were correctly classified into their appropriate group (pre-exercise, postexercise, or injured-joint samples). In horses with joint injuries, arthroscopic scores were positively correlated with radiographic scores, but neither score correlated with SF or serum CTX-II concentration. Conclusions: Results suggested that serum and SF CTX-II concentrations and SF:serum CTX-II ratio may be used to detect cartilage degradation in horses with joint injury.
Publication Date: 2010-01-02 PubMed ID: 20043778DOI: 10.2460/ajvr.71.1.33Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the effects of exercise and joint injuries on the presence of a specific type of collagen (type II) in the blood and joint fluid of thoroughbred racehorses. The findings suggest that this collagen’s presence can be used to detect cartilage damage in horses with joint injuries.
Introduction
- The study’s main objective was to determine the impact of physical exercise and osteochondral (bone and cartilage) injuries on the levels of carboxy-terminal telopeptide fragments of type II collagen (CTX-II) in synovial fluid and blood serum of Thoroughbred racehorses.
- The levels of CTX-II were compared with radiographic and arthroscopic scores, which are methods of evaluating the severity of joint injuries.
Methodology
- A total of 78 Thoroughbred racehorses, some with osteochondral injuries (38 horses) and some without (40 horses), were included in the study.
- Joint fluid and blood samples were collected both before and after a 5 to 6-month racing training from non-injured horses. From injured horses, samples were collected from the affected joint.
- These samples were analyzed using an Enzyme-Linked Immunosorbent Assay (ELISA) to determine CTX-II concentrations.
- The severity of joint injuries in the injured horses was evaluated through radiography and arthroscopy for comparison with CTX-II levels.
Results
- The study found significantly higher CTX-II levels in the joint fluid and a higher joint fluid-to-serum CTX-II ratio in injured horses as compared to pre- and post-exercise measurements in non-injured horses.
- Conversely, blood serum CTX-II concentrations were significantly lower in post-exercise and injured horse samples as compared to pre-exercise samples.
- Using the CTX-II measurements in serum and joint fluid, as well as the joint fluid-to-serum CTX-II ratio, 64% to 93% of samples were accurately categorized as pre-exercise, post-exercise, or joint-injured samples.
- Interestingly, while there was a positive correlation between arthroscopic and radiographic scores in the injured horses, these scores did not correlate with joint fluid or serum CTX-II concentration.
Conclusion
- According to the results, the concentration of CTX-II in joint fluid and blood serum, as well as the joint fluid-to-serum CTX-II ratio could serve as important indicators for detecting cartilage degradation in horses with joint injuries.
Cite This Article
APA
Cleary OB, Trumble TN, Merritt KA, Brown MP.
(2010).
Effect of exercise and osteochondral injury on synovial fluid and serum concentrations of carboxy-terminal telopeptide fragments of type II collagen in racehorses.
Am J Vet Res, 71(1), 33-40.
https://doi.org/10.2460/ajvr.71.1.33 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
MeSH Terms
- Animals
- Arthroscopy / veterinary
- Biomarkers / analysis
- Cartilage, Articular / injuries
- Collagen Type II / metabolism
- Horse Diseases / metabolism
- Horses / injuries
- Joint Diseases / metabolism
- Joint Diseases / veterinary
- Physical Conditioning, Animal / physiology
- Running
- Synovial Fluid / chemistry
Citations
This article has been cited 5 times.- Lee S, Baker ME, Clinton M, Taylor SE. Use of Omics Data in Fracture Prediction; a Scoping and Systematic Review in Horses and Humans. Animals (Basel) 2021 Mar 30;11(4).
- Bertuglia A, Pagliara E, Grego E, Ricci A, Brkljaca-Bottegaro N. Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career. BMC Vet Res 2016 Nov 8;12(1):246.
- Yarmola EG, Shah Y, Arnold DP, Dobson J, Allen KD. Magnetic Capture of a Molecular Biomarker from Synovial Fluid in a Rat Model of Knee Osteoarthritis. Ann Biomed Eng 2016 Apr;44(4):1159-69.
- Maninchedda U, Lepage OM, Gangl M, Hilairet S, Remandet B, Meot F, Penarier G, Segard E, Cortez P, Jorgensen C, Steinberg R. Development of an equine groove model to induce metacarpophalangeal osteoarthritis: a pilot study on 6 horses. PLoS One 2015;10(2):e0115089.
- Bertuglia A, Pallante M, Pagliara E, Valle D, Bergamini L, Bollo E, Bullone M, Riccio B. Determinants of joint effusion in tarsocrural osteochondrosis of yearling Standardbred horses. Front Vet Sci 2024;11:1389798.
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