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Home / Equine Research Bank / Reprod Domest Anim / Effect of feeding fescue seed containing ergot alkaloid toxi...
Reproduction in domestic animals = Zuchthygiene2012; 47(6); 1017-1026; doi: 10.1111/j.1439-0531.2012.02008.x

Effect of feeding fescue seed containing ergot alkaloid toxins on stallion spermatogenesis and sperm cells.

Abstract: The cellular effects of tall fescue grass-associated toxic ergot alkaloids on stallion sperm and colt testicular tissue were evaluated. This was a continuation of an initial experiment where the effects of toxic ergot alkaloids on the stallion spermiogram were investigated. The only spermiogram parameter in exposed stallions that was affected by the toxic ergot alkaloids was a decreased gel-free volume of the ejaculate. This study examined the effect of toxic ergot alkaloids on chilling and freezing of the stallion sperm cells. The effect of toxic ergot alkaloids on chilled extended sperm cells for 48 h at 5°C was to make the sperm cells less likely to undergo a calcium ionophore-induced acrosome reaction. The toxic ergot alkaloids had no effect on the freezability of sperm cells. However, if yearling colts were fed toxic ergot alkaloids, then the cytological analysis of meiotic chromosome synapsis revealed a significant increase in the proportion of pachytene spermatocytes showing unpaired sex chromosomes compared to control spermatocytes. There was little effect of ergot alkaloids on adult stallions, but there might be a significant effect on yearling colts.
© 2012 Blackwell Verlag GmbH.
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Publication Date: 2012-04-24 PubMed ID: 22524585PubMed Central: PMC3475766DOI: 10.1111/j.1439-0531.2012.02008.xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study aims to understand the impact of toxic ergot alkaloids, found in tall fescue grass, on the spermatogenesis and sperm cells of stallions and yearling colts.

Objective of the Study

  • The main objective of the research is to determine the cellular effects of toxic ergot alkaloids from tall fescue seeds on horse spermatogenesis and sperm cell survival.

Findings from the Previous Research

  • The previous experiment established a link between toxic ergot alkaloids and a decrease in the gel-free volume in the ejaculate of exposed stallions. This study is a follow-up on those initial observations and findings.

Current Study Observations

  • In the current research, it was observed that when stallion sperm cells are exposed to ergot alkaloids and chilled for 48 hours at a temperature of 5°C, the sperm cells were less likely to undergo a calcium-ionophore induced acrosome reaction. An acrosome reaction is an exocytotic process a sperm undergoes to penetrate and fertilize an egg.
  • Ergot alkaloids did not affect the ability of the sperm cells to survive the freezing process, maintaining their viability even after cryopreservation.

Effects on Yearling Colts

  • In yearling colts (young male horses), feeding them with ergot alkaloids led to notable changes. The researchers observed that there was a significant increase in the proportion of pachytene spermatocytes (a stage in the process of spermatogenesis) showing unpaired sex chromosomes compared to control spermatocytes. This suggests possible disruptions in normal sex chromosome pairing during meiosis in colts’ testes, which could have serious reproductive implications.

Implications of the Research

  • The study shows that while ergot alkaloids may have minimal effects on adult stallions, they might have significant repercussions on yearling colts. More investigations are proposed to fully understand these potential effects and reproductive health consequences.

Cite This Article

APA
Fayrer-Hosken R, Stanley A, Hill N, Heusner G, Christian M, De La Fuente R, Baumann C, Jones L. (2012). Effect of feeding fescue seed containing ergot alkaloid toxins on stallion spermatogenesis and sperm cells. Reprod Domest Anim, 47(6), 1017-1026. https://doi.org/10.1111/j.1439-0531.2012.02008.x

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 47
Issue: 6
Pages: 1017-1026

Researcher Affiliations

Fayrer-Hosken, R
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. rfh@uga.edu
Stanley, A
    Hill, N
      Heusner, G
        Christian, M
          De La Fuente, R
            Baumann, C
              Jones, L

                MeSH Terms

                • Animal Feed / analysis
                • Animal Nutritional Physiological Phenomena
                • Animals
                • Cryopreservation / veterinary
                • Diet / veterinary
                • Ergot Alkaloids / chemistry
                • Ergot Alkaloids / toxicity
                • Freezing
                • Horses / physiology
                • Male
                • Poaceae / chemistry
                • Seeds / chemistry
                • Semen
                • Semen Analysis / veterinary
                • Semen Preservation
                • Spermatogenesis / drug effects
                • Spermatozoa / cytology
                • Spermatozoa / drug effects

                Grant Funding

                • R01 HD042740 / NICHD NIH HHS
                • R56 HD093383 / NICHD NIH HHS
                • 2R01-HD042740 / NICHD NIH HHS

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