Effect of glucosamine on interleukin-1-conditioned articular cartilage.
Abstract: Glucosamine inhibits recombinant human interleukin-1 stimulated cartilage degradation in equine cartilage explants. Recently, recombinant equine interleukin-1 has been cloned and purified. Therefore, the objective of this study was to characterise the effects of glucosamine on indices of cartilage degradation in recombinant equine IL-1beta-stimulated equine articular cartilage explants. Cartilage discs were harvested from the weight-bearing region of the articular surface of the antebrachiocarpal and middle carpal joints of horses (age 2-8 years) and cultured under standard conditions. Explants were exposed to recombinant equine interleukin-1beta (reIL-1beta) on Days 1-4 in the presence or absence of glucosamine (0.25, 2.5 or 25 mg/ml), with appropriate controls. Nitric oxide, prostaglandin E2, sulphated proteoglycan, stromelysin and gelatinase/collagenase activity released into conditioned media and total tissue proteoglycan content were measured as indicators of cartilage catabolism. Glucosamine inhibited cartilage catabolic responses in a dose dependent manner that was statistically significant at a dose of 0.25 mg/ml for stromelysin activity and 2.5 mg/ml for collagenase/gelatinase activity. At 25 mg/ml glucosamine also prevented IL-1beta-induced increases in nitric oxide production, prostaglandin E2 and proteoglycan release to media. Glucosamine prevents equine articular cartilage degradation experimentally induced by reIL-1beta in vitro. These data provide further support for the use of glucosamine in treatment or prevention of cartilage loss in athletic horses.
Publication Date: 2002-10-31 PubMed ID: 12405690DOI: 10.1111/j.2042-3306.2002.tb05422.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research study explores how glucosamine affects the breakdown of cartilage in horses that have been exposed to a protein called recombinant equine interleukin-1beta. The research found that glucosamine significantly reduced this breakdown, supporting the use of this compound in treating or preventing cartilage loss in horses.
Objectives and Methodology:
- The primary aim of the study was to explore the impact of glucosamine on cartilage exposed to recombinant equine interleukin-1beta (reIL-1beta), a protein involved in cartilage degradation.
- Cartilage samples (disks) were collected from the joints of horses, aged 2-8 years old.
- These cartilage explants were exposed to reIL-1beta along with varying concentrations of glucosamine for a period of four days.
Measurement:
- Various indicators linked with cartilage breakdown, namely nitric oxide, prostaglandin E2, sulphated proteoglycan, stromelysin, and the combined activity of gelatinase and collagenase, were measured in the skillfully managed media of cartilage samples.
- The total tissue proteoglycan content was also checked as a part of experiments.
Findings:
- Glucosamine showed a significant inhibitory effect on the catabolic (destructive) responses within the cartilage. This inhibiting effect was dose-dependent, showing higher effectiveness with increased dosage.
- The statistically significant impact was observed at the 0.25 mg/ml for stromelysin activity and 2.5 mg/ml for the combined action of collagenase and gelatinase.
- At a higher dose of 25mg/ml, glucosamine prevented the interleukin-1beta-induced production of nitric oxide, discharge of prostaglandin E2 and proteoglycan into the media.
Implications:
- The findings demonstrate that glucosamine can potentially prevent degradation of articular cartilage induced by reIL-1beta.
- These results provide strong support for the use of glucosamine in the treatment or prevention of cartilage loss in athletic horses.
Cite This Article
APA
Fenton JI, Chlebek-Brown KA, Caron JP, Orth MW.
(2002).
Effect of glucosamine on interleukin-1-conditioned articular cartilage.
Equine Vet J Suppl(34), 219-223.
https://doi.org/10.1111/j.2042-3306.2002.tb05422.x Publication
Researcher Affiliations
- Department of Animal Science, Michigan State University, East Lansing 48824, USA.
MeSH Terms
- Animals
- Cartilage, Articular / drug effects
- Cartilage, Articular / metabolism
- Cells, Cultured
- Dose-Response Relationship, Drug
- Glucosamine / pharmacology
- Glucosamine / therapeutic use
- Horse Diseases / drug therapy
- Horse Diseases / metabolism
- Horses
- Interleukin-1 / pharmacology
- Matrix Metalloproteinase 3 / drug effects
- Matrix Metalloproteinase 3 / metabolism
- Matrix Metalloproteinase Inhibitors
- Matrix Metalloproteinases / drug effects
- Matrix Metalloproteinases / metabolism
- Nitric Oxide / biosynthesis
- Osteoarthritis / drug therapy
- Osteoarthritis / metabolism
- Osteoarthritis / veterinary
Citations
This article has been cited 3 times.- Pagani S, Minguzzi M, Sicuro L, Veronesi F, Santi S, Scotto D'Abusco A, Fini M, Borzì RM. The N-Acetyl Phenylalanine Glucosamine Derivative Attenuates the Inflammatory/Catabolic Environment in a Chondrocyte-Synoviocyte Co-Culture System.. Sci Rep 2019 Sep 19;9(1):13603.
- Uitterlinden EJ, Koevoet JL, Verkoelen CF, Bierma-Zeinstra SM, Jahr H, Weinans H, Verhaar JA, van Osch GJ. Glucosamine increases hyaluronic acid production in human osteoarthritic synovium explants.. BMC Musculoskelet Disord 2008 Sep 11;9:120.
- Gouze JN, Gouze E, Popp MP, Bush ML, Dacanay EA, Kay JD, Levings PP, Patel KR, Saran JP, Watson RS, Ghivizzani SC. Exogenous glucosamine globally protects chondrocytes from the arthritogenic effects of IL-1beta.. Arthritis Res Ther 2006;8(6):R173.
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