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BMC veterinary research2012; 8; 142; doi: 10.1186/1746-6148-8-142

Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue.

Abstract: Mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs) are being applied to equine cell therapy. The physiological environment in which MSCs reside is hypoxic and does not resemble the oxygen level typically used in in vitro culture (20% O2). This work compares the growth kinetics, viability, cell cycle, phenotype and expression of pluripotency markers in both equine BM-MSCs and AT-MSCs at 5% and 20% O2. Results: At the conclusion of culture, fewer BM-MSCs were obtained in hypoxia than in normoxia as a result of significantly reduced cell division. Hypoxic AT-MSCs proliferated less than normoxic AT-MSCs because of a significantly higher presence of non-viable cells during culture. Flow cytometry analysis revealed that the immunophenotype of both MSCs was maintained in both oxygen conditions. Gene expression analysis using RT-qPCR showed that statistically significant differences were only found for CD49d in BM-MSCs and CD44 in AT-MSCs. Similar gene expression patterns were observed at both 5% and 20% O2 for the remaining surface markers. Equine MSCs expressed the embryonic markers NANOG, OCT4 and SOX2 in both oxygen conditions. Additionally, hypoxic cells tended to display higher expression, which might indicate that hypoxia retains equine MSCs in an undifferentiated state. Conclusions: Hypoxia attenuates the proliferative capacity of equine MSCs, but does not affect the phenotype and seems to keep them more undifferentiated than normoxic MSCs.
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Publication Date: 2012-08-22 PubMed ID: 22913590PubMed Central: PMC3483288DOI: 10.1186/1746-6148-8-142Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the impact of low oxygen (hypoxia) on the growth and characteristics of horse-derived stem cells from bone marrow and fat tissue, typically used in horse stem cell therapy. They found that low oxygen suppressed the cells’ growth but it appeared to keep them in a more unaltered state.

Research Context and Objectives

  • This research was carried out in the context of stem cell therapy in horses, focusing on Mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs).
  • MSCs naturally reside in low-oxygen conditions (hypoxia), which is different from the high-oxygen conditions used in laboratory cell culture techniques.
  • The study aims to compare the growth rate, survival, cell cycle, phenotype, and expression of pluripotency markers in equine BM-MSCs and AT-MSCs under both low (5%) and high (20%) oxygen conditions.

Key Findings

  • Results of the study showed that fewer BM-MSCs were obtained in low oxygen conditions due to a significant reduction in cell division.
  • On the other hand, AT-MSCs proliferated less under low-oxygen conditions because of the higher number of non-surviving cells during culture.
  • Despite the differences in cell growth due to oxygen levels, both types of MSCs maintained their immunophenotype (set of antigens) under both conditions, meaning their identity didn’t change because of oxygen levels.
  • Gene expression analysis revealed statistically significant differences only in the expression of certain genes (CD49d in BM-MSCs and CD44 in AT-MSCs) under different oxygen conditions. For the remaining genes, similar expression patterns were observed at both oxygen levels.
  • Interestingly, all MSCs, regardless of their source and oxygen condition, expressed “embryonic markers” – genes (NANOG, OCT4, SOX2) typically found in less differentiated, more primitive cells. Moreover, cells in low oxygen tended to express these markers even more, indicating they might maintain a more stem cell-like, undifferentiated state under these conditions.

Conclusions

  • In conclusion, low oxygen levels suppress the proliferative capacity of horse MSCs but don’t change their phenotype or surface antigens.
  • Significantly, low-oxygen conditions appeared to keep the MSCs in a more undifferentiated, stem cell-like state, which could have implications for therapeutic strategies involving these cells.

Cite This Article

APA
Ranera B, Remacha AR, Álvarez-Arguedas S, Romero A, Vázquez FJ, Zaragoza P, Martín-Burriel I, Rodellar C. (2012). Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue. BMC Vet Res, 8, 142. https://doi.org/10.1186/1746-6148-8-142

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 8
Pages: 142

Researcher Affiliations

Ranera, Beatriz
  • Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain.
Remacha, Ana Rosa
    Álvarez-Arguedas, Samuel
      Romero, Antonio
        Vázquez, Francisco José
          Zaragoza, Pilar
            Martín-Burriel, Inmaculada
              Rodellar, Clementina

                MeSH Terms

                • Animals
                • Biomarkers
                • Bone Marrow Cells / cytology
                • Bone Marrow Cells / drug effects
                • Bone Marrow Cells / physiology
                • Cell Culture Techniques
                • Cell Division / drug effects
                • Cell Proliferation
                • Cell Survival
                • Gene Expression Regulation / drug effects
                • Membrane Proteins / genetics
                • Membrane Proteins / metabolism
                • Mesenchymal Stem Cells / cytology
                • Mesenchymal Stem Cells / drug effects
                • Mesenchymal Stem Cells / physiology
                • Oxygen / pharmacology

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