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Home / Equine Research Bank / Vet Clin Pathol / Effect of lactic acid addition to equine whole blood on plat...
Veterinary clinical pathology2022; 51(1); 65-69; doi: 10.1111/vcp.13034

Effect of lactic acid addition to equine whole blood on platelet aggregation measured by impedance aggregometry.

Abstract: Acidemia in sick or injured horses is often due to lactic acid accumulation. Alterations in platelet function and hemostasis are among numerous deleterious effects caused by decreased physiologic pH. Objective: We aimed to evaluate the effect of hyperlactatemia and resultant acidemia on platelet aggregation in equine whole blood using impedance aggregometry. Methods: Platelet aggregation was measured using the Multiplate analyzer in whole blood from 34 healthy horses at baseline and after in vitro addition of lactic acid to adjust the pH. Platelet aggregation of each sample was quantified by the area under the curve measurement reported by the Multiplate system. The association between platelet aggregation and pH was analyzed using a linear mixed-effects model. The association of baseline platelet aggregation with hematocrits (Hcts), white blood cell (WBC) counts, and platelet counts was evaluated using Pearson's correlations. Results: There was a significant association between acidemia and decreased platelet aggregation. No significant correlations were detected between platelet aggregation and Hct, WBC count, or platelet count. Platelet aggregation measured in healthy horses using the Multiplate analyzer showed substantial variation between animals. Conclusions: Acidemia caused by the addition of lactic acid to equine whole blood was associated with a mild though statistically significant decrease in platelet aggregation. In conjunction with other factors, this change may contribute to morbidity-related disorders of hemostasis, although its precise clinical relevance is uncertain.
© 2022 American Society for Veterinary Clinical Pathology.
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Publication Date: 2022-01-05 PubMed ID: 34989029DOI: 10.1111/vcp.13034Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how the addition of lactic acid to horse blood impacts platelet aggregation, with the results revealing that high levels of lactic acid can decrease platelet aggregation.

Study Purpose

  • The primary aim of this study was to explore how hyperlactatemia—a condition characterized by an excessive amount of lactic acid in the blood—and resultant acidemia impacted the aggregation of platelets within the blood of horses. The researchers used impedance aggregometry to assess this. Alterations in platelet function due to lower body pH can have numerous negative effects.

Methodology

  • The researchers drew whole blood samples from 34 healthy horses. They determined the aggregation rate of the platelets within this blood both at baseline and after artificially adding lactic acid to adjust pH levels.
  • The sample’s platelet aggregation was measured using a device called a Multiplate analyzer, which provided an area under the curve measurement to quantify aggregation.
  • To understand the relationship between platelet aggregation and pH levels, a linear mixed-effects model was applied.
  • In order to explore potential correlations between baseline platelet aggregation, hematocrit levels, white blood cell counts, and platelet counts, the researchers made use of Pearson’s correlation.

Findings

  • The study established a significant correlation between acidemia and a decrease in platelet aggregation. When the blood pH levels were lower due to higher levels of lactic acid, platelet aggregation rates diminished.
  • There was, however, no significant correlation found between platelet aggregation rates and the hematocrit levels, white blood cell count, or platelet count of the blood samples.
  • It was observed that platelet aggregation rates, when measured in healthy horses using the Multiplate analyzer, showed noticeable variations between animals.

Conclusion

  • The study concluded that acidemia, induced by adding lactic acid to equine whole blood, was associated with a mild but statistically significant decrease in the aggregation rate of platelets.
  • The researchers believed that, together with other factors, this change could contribute to disorders related to hemostasis which might further lead to various health-related disorders. However, the exact relevance of this finding in a clinical setting remains uncertain and requires more research.

Cite This Article

APA
Lanier CJ, Taintor JS, Christopherson PW, Spangler EA. (2022). Effect of lactic acid addition to equine whole blood on platelet aggregation measured by impedance aggregometry. Vet Clin Pathol, 51(1), 65-69. https://doi.org/10.1111/vcp.13034

Publication

Veterinary clinical pathology
ISSN: 1939-165X
NlmUniqueID: 9880575
Country: United States
Language: English
Volume: 51
Issue: 1
Pages: 65-69

Researcher Affiliations

Lanier, Christopher J
  • Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
Taintor, Jennifer S
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
Christopherson, Peter W
  • Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
Spangler, Elizabeth A
  • Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.

MeSH Terms

  • Animals
  • Blood Platelets
  • Electric Impedance
  • Horses
  • Lactic Acid
  • Platelet Aggregation
  • Platelet Count / veterinary
  • Platelet Function Tests / veterinary

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This article includes 20 references
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Citations

This article has been cited 2 times.
  1. Dervishi E, Bai X, Dyck MK, Harding JCS, Fortin F, Dekkers JCM, Plastow G. GWAS and genetic and phenotypic correlations of plasma metabolites with complete blood count traits in healthy young pigs reveal implications for pig immune response. Front Mol Biosci 2023;10:1140375.
    doi: 10.3389/fmolb.2023.1140375pubmed: 36968283google scholar: lookup
  2. Miglio A, Falcinelli E, Cappelli K, Mecocci S, Mezzasoma AM, Antognoni MT, Gresele P. Effect of Regular Training on Platelet Function in Untrained Thoroughbreds. Animals (Basel) 2024 Jan 27;14(3).
    doi: 10.3390/ani14030414pubmed: 38338057google scholar: lookup
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