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Animals : an open access journal from MDPI2022; 12(9); 1106; doi: 10.3390/ani12091106

Effect of Lentinula edodes on Morphological and Biochemical Blood Parameters of Horses.

Abstract: Shiitake mushrooms have been highly regarded as possessing enormous nutritive and medicinal values. No clinical studies have yet investigated the effect of shitake supplementation on the health of horses. The aim of this study was to evaluate the effect of shiitake mushroom supplementation on the morphological and biochemical blood properties in horses. A total of 17 adult horses were divided into two groups: supplemented and control. The supplemented group was fed 60 g of shiitake mushrooms per day for 5 months. Blood samples were collected in five sessions. Blood morphological analysis showed higher levels of lymphocytes in session 3 and monocytes in session 4 in the supplemented group. In addition, basophils, hemoglobin, and hematocrit were elevated compared to the control group. Biochemical analysis showed that the shiitake mushrooms affected a large number of parameters. In particular, alkaline phosphatase was found to be the most sensitive to shitake mushroom supplementation, for which the statistical differences were significant for sessions 2, 4, and 5. Furthermore, calcium was found to be affected by supplementation only in session 4, and gamma-glutamyl transferase in session 2. In addition, the bilirubin and glucose levels were lower in the supplemented group, and the albumin/globulin ratio was higher compared to the control group. The differences between the supplement and the control group in various sessions suggest that shiitake mushrooms are a beneficial nutritional supplement for horses.
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Publication Date: 2022-04-25 PubMed ID: 35565533PubMed Central: PMC9100334DOI: 10.3390/ani12091106Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the effects of shiitake mushroom supplementation on the blood properties of horses, demonstrating that it can have beneficial effects on both morphological and biochemical blood parameters.

Study Design

  • The study included 17 adult horses which were divided into two groups – a control group and a group that was given a daily supplement of 60g of shiitake mushrooms for 5 months.
  • The researchers collected blood samples from both groups on five different occasions throughout the study period.

Results on Morphological Blood Properties

  • The morphological analysis of the blood from the horses supplemented with shiitake showed heightened levels of lymphocytes during the third sample collection and monocytes during the fourth.
  • The levels of basophils, hemoglobin, and hematocrit were also higher in the supplemented horses compared to the control group.

Results on Biochemical Blood Parameters

  • The biochemical analysis indicated that shiitake mushrooms affected various substances in the horse’s blood.
  • The largest change was seen in the enzyme alkaline phosphatase which showed significant statistical differences in the second, fourth, and fifth blood samples collected from the supplemented group compared to the control group.
  • Other biochemical substances that showed changes include calcium, only during the fourth blood sampling session, and gamma-glutamyl transferase, which was affected only during the second session.
  • Supplementation also caused reduced levels of bilirubin and glucose in the blood of horses compared to the control group.
  • The ratio of albumin to globulin was found to be higher in the supplemented group relative to the control group, suggesting a positive impact on protein metabolism.

Conclusion

  • The various differences seen in both morphological and biochemical blood parameters between the supplemented and control group across the different sampling sessions indicates that shiitake mushrooms could have potential benefits as a nutritional supplement for horses.

Cite This Article

APA
Soroko M, Górniak W, Zielińska P, Górniak A, Śniegucka K, Nawrot K, Korczyński M. (2022). Effect of Lentinula edodes on Morphological and Biochemical Blood Parameters of Horses. Animals (Basel), 12(9), 1106. https://doi.org/10.3390/ani12091106

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 9
PII: 1106

Researcher Affiliations

Soroko, Maria
  • Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland.
Górniak, Wanda
  • Department of Automotive Engineering, Mechanical Faculty, Wroclaw University of Science and Technology, Na Grobli 13, 50-421 Wroclaw, Poland.
Zielińska, Paulina
  • Department of Surgery, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 51, 50-366 Wroclaw, Poland.
Górniak, Aleksander
  • Department of Automotive Engineering, Mechanical Faculty, Wroclaw University of Science and Technology, Na Grobli 13, 50-421 Wroclaw, Poland.
Śniegucka, Karolina
  • Department of Surgery, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 51, 50-366 Wroclaw, Poland.
Nawrot, Karolina
  • Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland.
Korczyński, Mariusz
  • Department of Animal Nutrition and Feed Management, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland.

Conflict of Interest Statement

Authors declare no conflict of interest.

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Citations

This article has been cited 4 times.
  1. Soroko-Dubrovina M, Górniak W, Zielińska P, Górniak A, Čebulj-Kadunc N, Korczyński M. Evaluation of Shiitake Mushroom (Lentinula edodes) Supplementation on the Blood Parameters of Young Thoroughbred Racehorses. Animals (Basel) 2022 Nov 19;12(22).
    doi: 10.3390/ani12223212pubmed: 36428438google scholar: lookup
  2. Qian W, Han J, Shi X, Qin X, Jiao F, Zhang M, Bao L, Su C. Mulberry (Morus alba) Twig and Leaf Extracts Ameliorate Obesity-Related Metabolic Disorders via Gut Microbiota Modulation in High-Fat Diet-Fed Mice. Animals (Basel) 2025 Jun 15;15(12).
    doi: 10.3390/ani15121768pubmed: 40564320google scholar: lookup
  3. Song W, Hu Y, Cheng M, Pu T, Niu W, Zhang Y, Liu X, Wang Y, Zhang Y, Liu J, Jin Y. Physiological and biochemical indicators in blood of captive yellow-cheeked gibbons and northern white-cheeked gibbons. BMC Vet Res 2025 May 20;21(1):363.
    doi: 10.1186/s12917-025-04797-7pubmed: 40389935google scholar: lookup
  4. Shen H, Wang M, Ning Y, Zhao Y, Danzeng B, Li K, Shi H, Li W. Effect of Dietary Addition of Lentinus edodes on Rumen Flora, Lactation, and Health of Dairy Goats. Animals (Basel) 2025 Feb 26;15(5).
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