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Frontiers in veterinary science2020; 7; 584922; doi: 10.3389/fvets.2020.584922

Effect of Meperidine on Equine Blood Histamine, Tryptase, and Immunoglobulin-E Concentrations.

Abstract: To evaluate changes in immunological parameters following subcutaneous (SC) and intramuscular (IM) administration of meperidine in horses through quantitative analysis of plasma tryptase, histamine, and IgE levels. Six adult horses were enrolled in a prospective randomized crossover design. Horses were administered one treatment per day, with a seven day washout period: (a) meperidine 1 mg/kg IM, saline 6 mL SC; (b) saline 6 mL IM, meperidine 1 mg/kg SC; (c) saline 6 mL SC, saline 6 mL IM. Blood samples were obtained for plasmatic histamine (baseline, 5, 10, 15, 30, and 60 min) via LC-MS/MS and plasmatic tryptase (baseline, 15, 30, 60, 120, and 240 min) quantification with enzyme-linked immunoabsorbent assays. Serum immunoglobulin E (IgE) concentrations prior to any meperidine treatment and 7-14 days following the first meperidine treatment were evaluated with enzyme-linked immunoabsorbent assays. Histamine and tryptase concentrations were evaluated with a mixed-effect analysis of variance. The levels of IgE at baseline (before the administration of the first dose of meperidine) were compared with the IgE values at 60 min following the second meperidine administration with the Paired test. Biopsies of localized injection site reactions from subcutaneous meperidine administration were collected from two horses. No statistically significant elevations from baseline in histamine ( = 0.595), tryptase ( = 0.836), or IgE ( = 0.844) were found in any of the horses in this study. There were no differences between treatment groups. Administration of SC meperidine caused a localized vasculitis and thrombosis with regional edema and hemorrhage. No evidence of anaphylactoid or anaphylactic type reactions occurred following IM or SC meperidine administration.
Copyright © 2020 Trenholme, Sakai, Berghaus, Hanafi, Knych, Ryan, McHale, Banovic, Quandt, Barletta and Reed.
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Publication Date: 2020-12-23 PubMed ID: 33426016PubMed Central: PMC7786019DOI: 10.3389/fvets.2020.584922Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines if the administration of meperidine, a type of medication, through subcutaneous (SC) and intramuscular (IM) routes in horses causes changes in the levels of some immune system parameters: histamine, tryptase, and IgE. The study results show that meperidine does not significantly increase these parameters, indicating it may not cause severe allergic reactions in horses. However, there was a local inflammation and blood clot development observed at the site of SC meperidine injection.

Study Design

  • The study involved six adult horses participating in a randomized crossover experiment where each horse experienced each treatment in different phases.
  • Three types of treatments were involved: meperidine given intramuscularly with saline subcutaneously; meperidine given subcutaneously with saline intramuscularly; and saline given both intramuscularly and subcutaneously.
  • The treatment was administered once a day with a seven-day washout period in between each phase.

Data Collection and Analysis

  • Blood samples were taken from the horses at various points in time depending on the parameter being measured: histamine was measured from baseline and every 5-60 minutes after treatment; tryptase was measured from baseline and after 15-240 minutes; and IgE was checked before any treatment and 7-14 days after the first meperidine treatment.
  • Various methods were used to evaluate these parameters: LC-MS/MS for histamine, enzyme-linked immunoabsorbent assays for tryptase and IgE.
  • For statistical analyses, histamine and tryptase concentrations were compared using a mixed-effect analysis of variance, while baseline IgE values and those after 60 minutes from the second meperidine administration were compared using the Paired t test.
  • Biopsies were also taken from two horses who showed localized reactions from the subcutaneous meperidine injection.

Findings

  • The results show that the administration of meperidine did not cause any significant increase in the levels of histamine, tryptase, and IgE in the horses.
  • There were no differences observed between the treatment groups in the levels of these parameters either.
  • Nevertheless, subcutaneous injection of meperidine led to localized inflammation and clot formation with accompanying swelling and bleeding in the area.

Conclusions

  • The study concludes that meperidine does not cause significant changes in the concentrations of histamine, tryptase, and IgE, suggesting it may not lead to severe allergic or anaphylactic reactions in horses.
  • However, a localized inflammation and clot formation was observed at the site of the subcutaneous meperidine injection, although it’s not clear how detrimental or significant this effect could be.

Cite This Article

APA
Trenholme HN, Sakai DM, Berghaus LJ, Hanafi AL, Knych HK, Ryan CA, McHale B, Banovic F, Quandt JE, Barletta M, Reed RA. (2020). Effect of Meperidine on Equine Blood Histamine, Tryptase, and Immunoglobulin-E Concentrations. Front Vet Sci, 7, 584922. https://doi.org/10.3389/fvets.2020.584922

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 7
Pages: 584922
PII: 584922

Researcher Affiliations

Trenholme, H Nicole
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Sakai, Daniel M
  • Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Berghaus, Londa J
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Hanafi, Amanda L
  • Peterson and Smith Equine Hospital, Ocala, FL, United States.
Knych, Heather K
  • K.L. Maddy Equine Analytical Pharmacology Laboratory, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
  • Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, CA, United States.
Ryan, Clare A
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
McHale, Brittany
  • Infectious Disease Laboratory, College of Veterinary Medicine, Department of Small Animal Medicine and Surgery, University of Georgia, Athens, GA, United States.
Banovic, Frane
  • Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Quandt, Jane E
  • Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Barletta, Michele
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.
Reed, Rachel A
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, United States.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Citations

This article has been cited 1 times.
  1. Yang NY, Ko JC, Wang HC, Liu PC. A preliminary study comparing the sedative, cardiorespiratory, and histaminic-releasing effects of intramuscular and intravenous administration of pethidine (meperidine) with midazolam in healthy cats. Vet Anim Sci 2021 Dec;14:100218.
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