Effect of Mycobacterium cell wall fraction on endometrial histomorphometry of mares resistant and susceptible to persistent breeding-induced endometritis.
Abstract: Mycobacterium cell wall fraction (MCWF) is a biological component made up of molecules with immunostimulant properties, which is therapeutically used to modulate persistent breeding-induced endometritis (PBIE). The aim of this study was to analyze the effect of this immunomodulator on the endometrial histological structure during the diestrus of PBIE-resistant and -susceptible mares that either received treatment with MCWF or not. The experiment was conducted with 10 resistant mares (RM) and 9 susceptible mares (SM). In the first estrous cycle of the trial, all mares were inseminated with dead semen as an inflammatory stimulus (Group A); at the next cycle, all mares were inseminated with dead semen and treated with a MCWF commercial immunomodulator (Group B). In both groups, endometrial biopsies were taken on day 7 post-ovulation (diestrus). Endometrial biopsies of untreated-RM (UTRM, n = 6), untreated-SM (UTSM, n = 7) MCWF-treated-RM (TRM, n = 6) and MCWF-treated-SM (TSM, n = 6) were evaluated. They were randomly chosen as representative mares of Group A and B, respectively. The height of lining and glandular epithelia, glandular diameter, glandular density and glandular area were evaluated. The histological structure revealed lymphocytic infiltration and dilated, tortuous glands with some glandular nests, particularly in UTSM. The histomorphometrical results showed no differences (ρ > 0.05) between the analyzed groups. This would indicate that post-service treatment with the MCWF immunomodulator does not modify the endometrial histoarchitecture but, apparently, its action would be mainly based on the stimulation of the cellular and humoral immune responses.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-06-30 PubMed ID: 32652325DOI: 10.1016/j.theriogenology.2020.06.034Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
- Veterinary
Summary
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The research article investigates the influence of Mycobacterium Cell Wall Fraction (MCWF) on persistent breeding-induced endometritis in mares, by studying structural changes in their endometrial tissues during different stages of the estrous cycle. The findings suggest that using MCWF as a treatment after insemination does not alter the structural make-up of the endometrium, but it possibly boosts cellular and humoral immune responses.
Methodology
- 19 mares, with 10 being resistant (RM) and 9 being susceptible (SM) to persistent breeding-induced endometritis (PBIE), were used in the experiment.
- The study was divided in to two cycles – all horses were inseminated with dead semen as an inflammatory stimulation in the first cycle (Group A).
- In the second cycle, all mares were also treated with an MCWF immunomodulator after being inseminated with dead semen (Group B).
- Endometrial biopsies were taken on the 7th day post-ovulation in both cycles, to assess the endometrial structure.
Analysis and Results
- The mares in the study were split into four categories – untreated-RM (UTRM), untreated-SM (UTSM), MCWF-treated-RM (TRM), and MCWF-treated-SM (TSM).
- The endometrial biopsy samples were examined for the height of lining and glandular epithelia, glandular diameter, glandular density, and glandular area.
- Lymphocytic infiltration and dilated, tortuous glands were observed in UTSM samples – these are indications of endometriosis.
- When comparing the untreated mares to the MCWF-treated mares, no significant histomorphological changes were identified.
Conclusion
- The study concludes that treatment with an MCWF immunomodulator after insemination does not alter the endometrial histology.
- The action of MCWF seems to stimulate cellular and humoral immune responses primarily, which could theoretically help to combat the inflammatory condition, instead of bringing about morphological changes to the endometrium.
Cite This Article
APA
Herrera MF, Otermin M, Herrera JM, Simoy MV, Bianchi CP, Aguilar JJ, Fumuso EA.
(2020).
Effect of Mycobacterium cell wall fraction on endometrial histomorphometry of mares resistant and susceptible to persistent breeding-induced endometritis.
Theriogenology, 156, 2-10.
https://doi.org/10.1016/j.theriogenology.2020.06.034 Publication
Researcher Affiliations
- Laboratorio de Histología y Embriología, Área de Ciencias Morfológicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Pje. Arroyo Seco S/N, CP B7000, Tandil, Buenos Aires, Argentina. Electronic address: mherrera@vet.unicen.edu.ar.
- Instituto Superior de Formación Docente Nº10, Belgrano 1610, CP B7000, Tandil, Buenos Aires, Argentina. Electronic address: otermin.m@hotmail.com.
- Laboratorio de Histología y Embriología, Área de Ciencias Morfológicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Pje. Arroyo Seco S/N, CP B7000, Tandil, Buenos Aires, Argentina. Electronic address: jumherrera@vet.unicen.edu.ar.
- Instituto Multidisciplinario sobre Ecosistemas y Desarrollo Sustentable, Facultad de Ciencias Exactas, Universidad Nacional del Centro de la Provincia de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Pje. Arroyo Seco S/N, CP B7000, Tandil, Buenos Aires, Argentina. Electronic address: vsimoy@gmail.com.
- Laboratorio de Endocrinología, Centro de Investigación Veterinaria Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Pje. Arroyo Seco S/N, CP B7000, Tandil, Buenos Aires, Argentina. Electronic address: cbianchi@vet.unicen.edu.ar.
- Producción Equina, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, CP 5800, Río Cuarto, Córdoba, Argentina. Electronic address: jaguilar@ayv.unrc.edu.ar.
- Laboratorio de Clínica y Reproducción Equina, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Pje. Arroyo Seco S/N, CP B7000, Tandil, Buenos Aires, Argentina. Electronic address: eafumuso@gmail.com.
MeSH Terms
- Animals
- Cell Wall
- Endometritis / veterinary
- Endometrium
- Female
- Horse Diseases
- Horses
- Mycobacterium
Conflict of Interest Statement
Declaration of competing interest The authors declare that they have no competing interests.
Citations
This article has been cited 2 times.- Vallejo-Soto P, Dorado J, Herrera-García R, Álvarez-Delgado C, Gómez-Laguna J, Santiago Á, Manrique M, González Ariza A, León Jurado JM, Hidalgo M, Ortiz I. Histomorphometric Analysis of the Endometrium of Jennies (Equus asinus) and Mares (Equus caballus) in Estrus: Anatomical Differences and Possible Reproductive Implications. Animals (Basel) 2026 Jan 4;16(1).
- Stanišić L, Stanimirović Z, Kovačević B, Blagojević J, Nedić S, Mašić A, Maletić M. The efficacy of intravenous application of the Mycobacterium Cell Wall Fraction (AMPLIMUNE) in the treatment of subclinical mastitis caused by S. Aureus. Vet Res Commun 2024 Aug;48(4):2429-2440.
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