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Veterinary journal (London, England : 1997)2005; 169(1); 65-74; doi: 10.1016/j.tvjl.2003.12.012

Effect of oral antioxidant supplementation on blood antioxidant status in trained thoroughbred horses.

Abstract: The oxidant/antioxidant equilibrium of trained thoroughbred horses (n = 40) was assessed on three occasions during a period of three months under field conditions by blood antioxidant markers analysis, i.e. plasma ascorbic acid (AA), plasma antioxidant capacity of water-soluble components (ACW), whole blood (GSH) and oxidised (GSSG) glutathione, plasma alpha-tocopherol and beta-carotene, plasma antioxidant capacity of lipid-soluble components (ACL), red blood cell superoxide dismutase (SOD) and glutathione-peroxidase activity (GPx) and plasma trace-elements, i.e. selenium (Se), copper (Cu), zinc (Zn). A control group of ten horses receiving a placebo and an antioxidant group of 30 horses orally supplemented with an antioxidant mixture were randomly formed. An antioxidant imbalance was observed after three months in the control group, reflected by a significant decrease in GSH, SOD, GPx, Se (P < 0.05) and a significant increase in GSSG (P < 0.05). The antioxidant supplement prevented GPx and Se decrease and significantly increased ACW, alpha-tocopherol, beta-carotene and ACL (P < 0.05). Significant sex- or age-related differences were found for AA, ACW, alpha-tocopherol, SOD, GPx and Se, and there were significant correlations between ACW-AA, ACL-alpha-tocopherol, GPx-Se, CPK-Se, CPK-alpha-tocopherol and CPK-Cu. This field study has shown that trained thoroughbred horses undergo significant changes of several blood antioxidant markers and that oral antioxidant supplementation might partially counterbalance these changes by improving the hydrophilic, lipophilic and enzymatic antioxidant blood capacity.
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Publication Date: 2005-02-03 PubMed ID: 15683765DOI: 10.1016/j.tvjl.2003.12.012Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on the effects of oral antioxidant supplementation on the oxidant/antioxidant balance in trained thoroughbred horses. Its findings indicate that such supplementation can help mitigate changes in several blood antioxidant markers, enhancing both the hydrophilic and lipophilic components of the antioxidants in the animals’ blood.

Research Assessment and Methodology

  • The research assesses the oxidant/antioxidant balance in trained thoroughbred horses over three months under field conditions.
  • The analysis was done using blood antioxidant markers, specifically plasma ascorbic acid, plasma antioxidant capacity of water-soluble components, whole blood and oxidised glutathione, plasma alpha-tocopherol and beta-carotene, plasma antioxidant capacity of lipid-soluble components, red blood cell superoxide dismutase and glutathione-peroxidase activity and plasma trace-elements like selenium, copper, and zinc.
  • Two groups were formed, a control group of ten horses receiving a placebo, and a test group of 30 horses who were orally supplemented with an antioxidant mixture.

Key Findings

  • The research showed that after three months, there was a significant antioxidant imbalance in the control group. This manifested as a significant decrease in glutathione, superoxide dismutase, glutathione-peroxidase activity, and selenium levels, and a significant increase in oxidised glutathione.
  • On the contrary, the group of horses given the antioxidant supplement showed prevention of glutathione-peroxidase activity and selenium decrease. This group also showed notable increases in the plasma antioxidant capacity of water-soluble components, alpha-tocopherol, beta-carotene, and the plasma antioxidant capacity of lipid-soluble components.
  • Sex- or age-related differences were noticed for different markers, and there were also significant correlations between several pairs of markers, like for instance between the antioxidant capacity of water-soluble components and ascorbic acid and between glutathione-peroxidase activity and selenium.

Conclusion

  • The research concludes that trained thoroughbred horses show substantial changes in their blood antioxidant markers. Successful mitigation of this change can be achieved by oral antioxidant supplementation, which can enhance the hydrophilic, lipophilic, and enzymatic antioxidant blood capacity.

Cite This Article

APA
de Moffarts B, Kirschvink N, Art T, Pincemail J, Lekeux P. (2005). Effect of oral antioxidant supplementation on blood antioxidant status in trained thoroughbred horses. Vet J, 169(1), 65-74. https://doi.org/10.1016/j.tvjl.2003.12.012

Publication

Veterinary journal (London, England : 1997)
ISSN: 1090-0233
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 169
Issue: 1
Pages: 65-74

Researcher Affiliations

de Moffarts, B
  • Department for Functional Sciences, Faculty of Veterinary Medicine, University of Liège, Sart Tilman, Bât B42, B-4000 Liège, Belgium.
Kirschvink, N
    Art, T
      Pincemail, J
        Lekeux, P

          MeSH Terms

          • Administration, Oral
          • Animals
          • Antioxidants / administration & dosage
          • Antioxidants / metabolism
          • Biomarkers / blood
          • Biomarkers / metabolism
          • Dietary Supplements
          • Female
          • Glutathione / blood
          • Glutathione / metabolism
          • Glutathione Peroxidase / blood
          • Glutathione Peroxidase / metabolism
          • Horses / blood
          • Horses / metabolism
          • Male
          • Minerals / blood
          • Minerals / metabolism
          • Random Allocation
          • Superoxide Dismutase / blood
          • Superoxide Dismutase / metabolism
          • Vitamins / blood
          • Vitamins / metabolism

          Citations

          This article has been cited 15 times.
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