Effect of ovarian hormones on promotion of bactericidal activity by uterine secretions of ovariectomized mares.
Abstract: The bactericidal and phagocytic activities of blood neutrophils suspended in uterine washings and the mobilization of neutrophils into the uterine lumen were studied in ovariectomized mares receiving oestradiol benzoate (N = 4), progesterone (N = 4) or oily vehicle (N = 4). Uterine lavage was performed sequentially up to 144 h after induction of endometritis by intrauterine infusion of glycogen (1%). There was no significant difference between the 3 groups in speed of mobilization of neutrophils into the uterus in the first 6 h after infusion but there were significantly more uterine luminal neutrophils in progesterone-treated than in oestradiol-treated mares by 24 h after infusion (P less than 0.01). Uterine washings collected from progesterone-treated mares at 0, 24 and 144 h were significantly worse at promoting bactericidal activity by neutrophils than washings from oestradiol-treated and control mares (P less than 0.001). In oestrogen-treated and control mares bactericidal activity had increased by 144 h but in progesterone-treated mares bactericidal activity remained low. Neither treatment nor time affected the ability of washings to opsonize yeast blastospores. Elevated concentrations of progesterone in plasma were therefore associated with decreased bactericidal activity of neutrophils suspended in uterine washings but the generation of C3b in washings did not appear to be affected by hormone treatment.
Publication Date: 1987-03-01 PubMed ID: 3572884DOI: 10.1530/jrf.0.0790531Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research conducted a study on horses that were ovariectomized to examine the effect of ovarian hormones on the bactericidal action of uterine secretions. It was found that while estrogen-related hormones showed an increase in bactericidal activity over time, the introduction of progesterone resulted in a consistent low bactericidal activity even after a significant period.
Objective of the Study and Method
- This study aimed to investigate the influence of ovarian hormones on the bactericidal and phagocytic activities of blood neutrophils in the uterine secretions of ovariectomized mares.
- The subjects of the study were mares that were given either oestradiol benzoate, progesterone, or an oily vehicle.
- Endometritis was induced in each subject via intrauterine infusion of glycogen, followed by the sequential collection of uterine washings up to 144 hours.
Findings and Interpretations
- The study found no significant difference in the speed of neutrophil mobilization into the uterus within the first 6 hours post-infusion between the three groups.
- There were significantly more uterine luminal neutrophils in the progesterone-treated horses than those treated with oestradiol by 24 hours post-infusion.
- Bactericidal activity was severely limited in the uterine washings from progesterone-treated mares, compared to oestradiol-treated and control mares.
- In estrogen-treated and control mares, bactericidal activity had increased by 144 hours, but it remained low in progesterone-treated mares.
- The ability of washings to opsonize yeast blastospores was not affected by either the treatment type or time.
- Increased levels of progesterone in the plasma were associated with decreased bactericidal activity of neutrophils in uterine washings, but the production of C3b, an integral part of the immune response, was not affected by hormone treatment.
Conclusion
- The results of this study suggest that the presence of ovarian hormones, particularly progesterone, can potentially suppress the bactericidal activity of the uterine secretions.
- While the exact effect of these hormones on the immune response remains uncertain, the significant reduction in the bactericidal activity can potentially increase the risk of infections in the uterus.
Cite This Article
APA
Watson ED, Stokes CR, David JS, Bourne FJ.
(1987).
Effect of ovarian hormones on promotion of bactericidal activity by uterine secretions of ovariectomized mares.
J Reprod Fertil, 79(2), 531-537.
https://doi.org/10.1530/jrf.0.0790531 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Blood Bactericidal Activity
- Estradiol / pharmacology
- Female
- Horses
- Neutrophils / physiology
- Ovariectomy
- Phagocytosis
- Progesterone / pharmacology
- Streptococcus / drug effects
Citations
This article has been cited 4 times.- Adnane M, Chapwanya A. Role of Genital Tract Bacteria in Promoting Endometrial Health in Cattle. Microorganisms 2022 Nov 12;10(11).
- Nash DM, Paddison J, Davies Morel MCG, Barnea ER. Preimplantation factor modulates acute inflammatory responses of equine endometrium. Vet Med Sci 2018 Nov;4(4):351-356.
- Hinrichs K, Riera FL, Klunder LR. Establishment of pregnancy after embryo transfer in mares with gonadal dysgenesis. J In Vitro Fert Embryo Transf 1989 Oct;6(5):305-9.
- Adnane M, Chapwanya A. Microbial Gatekeepers of Fertility in the Female Reproductive Microbiome of Cattle. Int J Mol Sci 2024 Oct 10;25(20).
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