Effect of ovary storage and oocyte transport method on maturation rate of horse oocytes.
Abstract: Two experiments were conducted to determine the effects of storage on equine ovaries or isolated oocytes. Ovaries were collected at an abattoir and were maintained at room temperature during collection and transport (3-9h total). After arrival at the laboratory, ovaries were divided into three groups: immediate oocyte collection (control), storage at room temperature overnight (15-18 h) before oocyte collection, or storage at 4 degrees C overnight before oocyte collection. Collected oocytes were cultured in maturation medium for 24h. There was a significant increase in the proportion of oocytes classified as having compact cumuli in the two storage groups when compared with the controls. For oocytes originally having expanded cumuli, the rate of maturation to MII was significantly higher in the control group (72%) than in either storage group, and the maturation rate for oocytes from ovaries stored at room temperature (27%) was significantly higher than that for ovaries stored at 4 degrees C (10%). A similar trend was seen for oocytes originally having compact cumuli (24, 11, and 3% in MI-II for control, room temperature, and cold groups, respectively). In Experiment 2, we evaluated the effect of different packaging systems on the maturation of horse oocytes within a portable incubator. Use of 1 ml of equilibrated maturation medium in a 1 ml glass vial was associated with maturation equivalent to that for standard incubation.
Publication Date: 2003-01-09 PubMed ID: 12517380DOI: 10.1016/s0093-691x(02)01133-0Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article discusses the impact of different ovary storage conditions and oocyte transportation systems on the maturation capacity of horse oocytes. The study found significant variations in the maturation rates across the different storage and transportation setups, indicating their influence on the oocyte maturation process.
Objective of the study
- The primary purpose of the research was to investigate the impact of ovary storage and the method of oocyte transportation on the rate of horse oocyte maturation.
Methods and experimental design
- The study involved two separate experiments. In the first one, equine ovaries were collected and kept at room temperature during collectin and transport, which lasted 3-9 hours in total.
- Upon reaching the lab, the ovaries were classified into three groups based on the subsequent storage conditions: immediate oocyte collection (control), storage at room temperature overnight before oocyte collection, or refrigerated storage at 4 degrees Celsius overnight before oocyte extraction.
- The collected oocytes were then cultivated in a maturation medium for a 24-hour period.
- The second experiment was focused on studying the effect of different packaging systems on the maturation process within a portable incubator.
Results and observations
- A significant increase in the ratio of oocytes having compact cumuli was observed in the two storage conditions compared to the control.
- For oocytes initially having expanded cumuli, a significantly higher maturation rate was recorded for the control group (72%) compared to the two storage conditions.
- Furthermore, the matuturation rate of oocytes originating from ovaries stored at room temperature (27%) was significantly higher than that from ovaries stored in the refrigerated condition (10%).
- The same trend was observed for oocytes initially having compact cumuli in regards to their maturation rates across the three groups.
- The second experiment showed that using 1 ml of equilibrated maturation medium in a 1 ml glass vial was associated with a maturation rate equivalent to that of standard incubation.
Conclusions and implications
- Based on the study findings, it’s evident that the ovary storage conditions and the method of oocyte transportation significantly influence the maturation process of horse oocytes.
- The research could guide in establishing the optimal conditions for ovary storage and oocyte transportation, which could be integral for effective horse breeding practices, especially in vitro fertilization (IVF) procedures.
Cite This Article
APA
Love LB, Choi YH, Love CC, Varner DD, Hinrichs K.
(2003).
Effect of ovary storage and oocyte transport method on maturation rate of horse oocytes.
Theriogenology, 59(3-4), 765-774.
https://doi.org/10.1016/s0093-691x(02)01133-0 Publication
Researcher Affiliations
- Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
MeSH Terms
- Abattoirs
- Animals
- Cell Division
- Female
- Horses / physiology
- Metaphase / physiology
- Oocytes / cytology
- Oocytes / physiology
- Ovary / cytology
- Ovary / physiology
- Temperature
- Time Factors
- Tissue Preservation / methods
- Tissue Preservation / standards
- Tissue Preservation / veterinary
- Tissue and Organ Harvesting / methods
- Tissue and Organ Harvesting / veterinary
Citations
This article has been cited 2 times.- Hosoe M, Furusawa T, Noguchi J, Tokunaga T. Growth of follicles of various animals following ovarian grafting under the kidney capsules of immunodeficient mice. Reprod Med Biol 2008 Mar;7(1):45-54.
- Abdel-Khalek EA, El-Harairy MA, Shamiah ShM, Khalil WA. Effect of ovary preservation period on recovery rate and categories of dromedary camel oocytes. Saudi J Biol Sci 2010 Jul;17(3):231-5.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
