Effect of passive immunization against inhibin on FSH secretion, folliculogenesis and ovulation rate during the follicular phase of the estrous cycle in mares.
Abstract: Physiological roles of inhibin in mares were investigated by means of passive immunization using an antiserum to inhibin that had been raised in a castrated goat. Eight mares were given an intravenous injection of either 100 mL (n = 4) or 200 mL (n = 4) of inhibin antiserum 4 d after a single intramuscular injection of PGF2 alpha on Day 8 after ovulation, 4 control mares were treated with 100 mL castrated goat serum in the same manner. Jugular vein blood samples were collected after treatment with the serum until 192 h post treatment. Follicular growth and ovulations were monitored by ultrasound examination at 24-h intervals. The ability of the inhibin antiserum to neutralize the bioactivity of equine inhibin was examined in vitro using a rat pituitary cell culture system. Suppression of secretion of FSH from cultured rat pituitary cells by equine follicular fluid was reversed by the addition of increasing doses of the inhibin antiserum, thereby indicating its bioactivity. Plasma levels of FSH and estradiol-17 beta were higher in mares treated with the inhibin antiserum. The ovulation rate was significantly higher in mares treated with antiserum (100 mL = 3.75 +/- 0.63; 200 mL = 4.50 +/- 0.65) than in control mares (1.25 +/- 0.25). These results demonstrate that inhibin is important in regulating FSH secretion and folliculogenesis in mares. They also show that neutralization of the bioactivity of inhibin may become a new method for the control of folliculogenesis and ovulation rate in mares.
Publication Date: 2000-03-25 PubMed ID: 10732146DOI: 10.1016/s0093-691x(98)00160-5Google Scholar: Lookup
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- Journal Article
Summary
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This study aimed to understand the physiological roles of inhibin in mares and its potential effects on FSH secretion, folliculogenesis, and ovulation rate, using passive immunization and inhibin antiserum originated from a castrated goat.
Study Design and Methodology
- This experiment focused on eight mares administered with an intravenous injection of either 100 mL (4 mares) or 200 mL (4 mares) of inhibin antiserum. The dosage was given four days after a single intramuscular injection of PGF2 alpha on Day 8 after ovulation.
- As a control group, 4 mares were treated with a similar dose (100 mL) of castrated goat serum.
- Jugular vein blood samples were collected after the serum treatment until 192 hours post treatment.
- Follicular growth and ovulation events were monitored using ultrasound examination at 24-hour intervals.
- Additionally, the bioactivity of the inhibin antiserum was tested in vitro using a rat pituitary cell culture system.
Findings
- The inhibin antiserum effectively neutralized the bioactivity of equine inhibin, tested in the rat pituitary cell culture system. When the inhibin antiserum was added to the cell cultures, suppression of FSH secretion by equine follicular fluid was reversed.
- Plasma levels of FSH and estradiol-17 beta were found to be higher in mares treated with the inhibin antiserum than in the control group.
- The ovulation rate was significantly higher in mares treated with the antiserum (3.75 ± 0.63 for 100mL dosage and 4.50 ± 0.65 for 200mL dosage) compared to the control mares (1.25 ± 0.25).
Implications and Conclusions
- The results indicate that inhibin plays a significant role in regulating FSH secretion and folliculogenesis in mares.
- The study findings suggest that neutralizing inhibin’s bioactivity could become a novel way to control folliculogenesis and ovulation rate in mares.
Cite This Article
APA
Nambo Y, Kaneko H, Nagata S, Oikawa M, Yoshihara T, Nagamine N, Watanabe G, Taya K.
(2000).
Effect of passive immunization against inhibin on FSH secretion, folliculogenesis and ovulation rate during the follicular phase of the estrous cycle in mares.
Theriogenology, 50(4), 545-557.
https://doi.org/10.1016/s0093-691x(98)00160-5 Publication
Researcher Affiliations
- Clinical Science and Pathobiology Division, Japan Racing Association, Tochigi, Japan.
MeSH Terms
- Animals
- Estradiol / blood
- Female
- Follicle Stimulating Hormone / blood
- Follicle Stimulating Hormone / metabolism
- Horses / physiology
- Immunization, Passive
- Inhibins / immunology
- Inhibins / physiology
- Luteinizing Hormone / blood
- Ovarian Follicle / diagnostic imaging
- Ovarian Follicle / physiology
- Ovulation
- Progesterone / blood
- Ultrasonography
Citations
This article has been cited 13 times.- Kageyama A, Tsuchiya M, Terakawa J, Ito J, Kashiwazaki N. A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice. J Reprod Dev 2023 Aug 11;69(4):223-226.
- Hasegawa A, Mochida K, Nakamura A, Miyagasako R, Ohtsuka M, Hatakeyama M, Ogura A. Use of anti-inhibin monoclonal antibody for increasing the litter size of mouse strains and its application to in vivo-genome editing technology†. Biol Reprod 2022 Aug 9;107(2):605-618.
- Mochida K. Development of assisted reproductive technologies in small animal species for their efficient preservation and production. J Reprod Dev 2020 Aug 20;66(4):299-306.
- Wuri L, Agca C, Agca Y. Morphometric, subcellular, in vitro fertilisation and embryonic developmental assessment of mouse oocytes produced by anti-inhibin serum or pregnant mare serum gonadotrophin superovulation. Reprod Fertil Dev 2020 Mar;32(5):474-483.
- Huang Z, Zhang J, Hazihan W, Cai Z, Xin G, Feng X, Gu Y. Cloning expression and immunogenicity analysis of inhibin gene in Ye Mule Aries sheep. PeerJ 2019;7:e7761.
- Takeo T, Nakagata N. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. PLoS One 2015;10(5):e0128330.
- Tsukada T, Sato K, Moriyoshi M, Koyago M, Sawamukai Y. Treatment with a High Dose Combination of PMSG/hCG Preparation of Mares Clinically Diagnosed with Ovarian Quiescence during the Breeding Season (Investigation from 1975 to 2000). J Equine Sci 2008;19(2):35-8.
- Ward MA. Wild-derived inbred mice no longer ART-resistant. Biol Reprod 2012 May;86(5):168, 1-2.
- Steckler TL, Lee JS, Ye W, Inskeep EK, Padmanabhan V. Developmental programming: exogenous gonadotropin treatment rescues ovulatory function but does not completely normalize ovarian function in sheep treated prenatally with testosterone. Biol Reprod 2008 Oct;79(4):686-95.
- Medan MS, Nambo Y, Nagamine N, Shinbo H, Watanabe G, Groome N, Taya K. Plasma concentrations of ir-inhibin, inhibin A, inhibin pro-alphaC, FSH, and estradiol-17beta during estrous cycle in mares and their relationship with follicular growth. Endocrine 2004 Oct;25(1):7-14.
- Medan MS, Wang H, Watanabe G, Suzuki AK, Taya K. Immunization against endogenous inhibin increases normal oocyte/embryo production in adult mice. Endocrine 2004 Jul;24(2):115-9.
- Ueno E, Watanabe M, Kondo Y, Nakagata N, Takeo T, Nakao S, Ogiwara K. 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia. iScience 2025 Feb 21;28(2):111846.
- Mochida K, Morita K, Sasaoka Y, Morita K, Endo H, Hasegawa A, Asano M, Ogura A. Superovulation with an anti-inhibin monoclonal antibody improves the reproductive performance of rat strains by increasing the pregnancy rate and the litter size. Sci Rep 2024 Apr 26;14(1):8294.
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