Effect of periovulatory prostaglandin F2alpha on pregnancy rates and luteal function in the mare.
Abstract: The objective of this study was to determine whether periovulatory treatments with PGF2alpha affects the development of the CL, and whether the treatment was detrimental to the establishment of pregnancy. Reproductively sound mares were assigned randomly to one of the following treatment groups during consecutive estrus cycles: 1. 3,000 IU hCG within 24 hours before artificial insemination and 500 microg cloprostenol (PGF2alpha analogue) on Days 0, 1, and 2 after ovulation (n=8), 2. 2 mL sterile water injection within 24 hours before artificial insemination and 500 microg cloprostenol on Days 0, 1, and 2 after ovulation (n=8); 3. 3,000 IU hCG within 24 hours before artificial insemination and 500 microg cloprostenol on Day 2 after ovulation (n=8); or 4. 3,000 IU hCG within 24 hours before artificial insemination and 2 mL of sterile water on Days 0, 1, and 2 after ovulation (controls; n=8). Blood samples were collected from the jugular vein on Days 0, 1, 2, 5, 8, 11, and 14 after ovulation. Plasma progesterone concentrations were determined by the use of a solid phase 125I radioimmunoassay. All mares were examined for pregnancy by the use of transrectal ultrasonography at 14 days after ovulation. Mares in Group 1 and 2 had lower plasma progesterone concentrations at Day 2 and 5, compared to mares in the control group (P < 0.001). No difference was detected between group 1 and 2. Plasma progesterone concentrations in group 3 were similar to the control group until the day of treatment, but decreased after treatment and were significantly lower than the control group at Day 5 (P < 0.001). Plasma progesterone concentrations increased in all treatment groups after Day 5, and were comparable among all groups at Day 14 after ovulation. Cloprostenol treatment had a significant effect on pregnancy rates (P < 0.01). The pregnancy rate was 12.5% in Group 1, 25% in Group 2, 38% in Group 3, and 62.5% in Group 4. It was concluded that periovulatory treatment with PGF2alpha has a detrimental effect on early luteal function and pregnancy.
Publication Date: 2001-06-21 PubMed ID: 11414493DOI: 10.1016/s0093-691x(01)00530-1Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explores the effect of periovulatory treatments with prostaglandin F2alpha (PGF2alpha) on the establishment of pregnancy in mares. The study finds that PGF2alpha treatment disrupts early luteal function and reduces pregnancy rates.
Objective of Research
- The study aimed to determine the effects of periovulatory treatments with PGF2alpha on the development of the corpus luteum (CL), a structure that produces progesterone which is pivotal for the establishment and maintenance of pregnancy in mares.
Methodology
- The researchers conducted an experiment involving mares which were divided into four groups and treated differently. The group one mares received 3,000 IU hCG before artificial insemination and 500 micrograms of cloprostenol, a PGF2alpha analogue, on Days 0, 1 and 2 post-ovulation. The treatments for group two, three and four varied subtly, with alterations in the timing of cloprostenol administration and/or the replacement of hCG or cloprostenol with sterile water injections.
- Blood samples from the mares were collected on various days post-ovulation and plasma progesterone levels were analysed to assess luteal function. Transrectal ultrasonography was performed on Day 14 post-ovulation to assess pregnancy status.
Findings
- The research found that mares in groups that received cloprostenol treatment around the time of ovulation had lower plasma progesterone concentrations on Day 2 and Day 5 compared to the control group (group 4). This suggests that the cloprostenol treatment disrupted the early function of the corpus luteum.
- Progesterone concentrations in all the treatment groups returned to normal by Day 14 post-ovulation, indicating recovery of luteal function.
- Significantly, the groups treated with cloprostenol showed lower pregnancy rates compared to the control group, indicating a negative impact of this treatment on the establishment of pregnancy. The highest pregnancy rate was observed in the control group (62.5%), while group 1, 2 and 3 had pregnancy rates of 12.5%, 25%, and 38% respectively.
Conclusion
- The study concludes that periovulatory treatment with PGF2alpha, as represented by cloprostenol, negatively affects early luteal function and reduces the likelihood of successful pregnancy establishment in mares. Therefore, the use of this treatment should be carefully evaluated in terms of its potential benefits against its adverse effects on reproduction.
Cite This Article
APA
Troedsson MH, Ababneh MM, Ohlgren AF, Madill S, Vetscher N, Gregas M.
(2001).
Effect of periovulatory prostaglandin F2alpha on pregnancy rates and luteal function in the mare.
Theriogenology, 55(9), 1891-1899.
https://doi.org/10.1016/s0093-691x(01)00530-1 Publication
Researcher Affiliations
- Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
MeSH Terms
- Animals
- Breeding / methods
- Cloprostenol / pharmacology
- Corpus Luteum / drug effects
- Corpus Luteum / physiology
- Female
- Horses / physiology
- Insemination, Artificial / methods
- Insemination, Artificial / veterinary
- Ovulation
- Pregnancy
- Pregnancy Rate
- Pregnancy, Animal / drug effects
- Progesterone / blood
- Radioimmunoassay / veterinary
- Random Allocation
- Sperm Motility
Citations
This article has been cited 5 times.- Segabinazzi LGTM, Roberts BN, Peterson EW, Ambrosia R, Bergfelt D, Samper J, French H, Gilbert RO. Early Pregnancy in Jennies in the Caribbean: Corpus Luteum Development and Progesterone Production, Uterine and Embryo Dynamics, Conceptus Growth and Maturation. Animals (Basel) 2022 Jan 6;12(2).
- Niikura T, Tsogtgerel M, Niikura C, Wada H, Kanzawa S, Fujiwara T, Nambo Y. Effect of prostaglandin F2(α) administration on uterine polymorphonuclear neutrophil counts in Japanese heavy draft horses. J Equine Sci 2021 Dec;32(4):117-124.
- Segabinazzi LGTM, Canisso IF, Podico G, Cunha LL, Novello G, Rosser MF, Loux SC, Lima FS, Alvarenga MA. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics (Basel) 2021 Apr 23;10(5).
- Okada CTC, Kaps M, Perez Quesada J, Gautier C, Aurich J, Aurich C. Diestrous Ovulations in Pregnant Mares as a Response to Low Early Postovulatory Progestogen Concentration. Animals (Basel) 2020 Nov 30;10(12).
- Kalpokas I, Perdigón F, Rivero R, Talmon M, Sartore I, Viñoles C. Effect of a povidone-iodine intrauterine infusion on progesterone levels and endometrial steroid receptor expression in mares. Acta Vet Scand 2010 Dec 16;52(1):66.
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