Effect of platelet lysate on uterine response of mares susceptible to persistent mating-induced endometritis.
Abstract: Many mares are susceptible to persistent mating-induced endometritis (PMIE), an important cause of reduced fertility. Platelet lysate (PL) derives from freeze-thawing platelets after concentration, so that growth factors are released from the platelets. Among the advantages of PL compared to platelet-rich plasma (PRP), it can be frozen stored and allogenic use for PL might also be conceivable. Platelet-rich plasma beneficially reduced inflammatory response in PMIE mares when administered 24 h pre- or 4 h post-AI. The aim of this study was to test the effect of PL on inflammatory uterine response in mares susceptible to PMIE. A total of 14 mares susceptible to PMIE (based on presence of fluid or inflammatory cells 24 h after AI) underwent an untreated (Ctr) cycle followed by a treated (PL) cycle. From each mare, 100 mL of citrated whole blood was obtained for PRP production by centrifugation. The resultant PRP was brought to a final volume of 10 mL with platelet poor plasma and frozen at -80 °C to obtain PL. On untreated cycles, mares were inseminated with frozen-thawed semen 36 h after ovulation induction. On treated cycles, PL was thawed, infused into the uterus 12 h after ovulation induction, and AIs were performed 24 h later. The number of neutrophils in uterine cytology (score 1(normal)-3(severe inflammation)) evaluated by optical microscopy, uterine fluid accumulation (height x width) and uterine edema (score 0-3) observed in ultrasonography, were analysed. Pregnancy was evaluated by ultrasonography 14 days after ovulation. A significant decrease (P < 0.05) was observed on cytology score (PL 1.3 ± 0.1 vs Ctr 2.0 ± 0.1), fluid accumulation (PL 79.5 ± 30.1 mm2 vs Ctr 342.7 ± 52.9 mm2) and edema score (PL 1.8 ± 0.2 vs Ctr 2.3 ± 0.2) in treated mares. Pregnancy rate in PL-treated cycles (3/12) and control cycles (2/14), were not significantly different (P > 0.05). According to the results, we conclude that treatment with PL in mares classified as susceptible to PMIE appears to reduce the inflammatory response after breeding, based on clinical signs of uterine edema, IUF accumulation and PMNs migration.
Copyright © 2021 Elsevier Inc. All rights reserved.
Publication Date: 2021-12-02 PubMed ID: 34894489DOI: 10.1016/j.theriogenology.2021.12.001Google Scholar: Lookup
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Summary
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This research studied the effect of platelet lysate on the inflammation in mares susceptible to a persistent form of horse endometritis induced by mating. It found that the lysate decreased inflammation measures, though it did not significantly change pregnancy rates.
Study Overview
- The study focused on diagnosing the impact of platelet lysate (PL), a product derived from freeze-thawed concentrated platelets, on the uterine response of mares, specifically those vulnerable to persistent mating-induced endometritis (PMIE).
- A total of 14 mares susceptible to PMIE were part of this study. Diagnostic criteria included the presence of fluid or inflammatory cells in the uterus 24 hours after artificial insemination (AI).
- Platelet-rich plasma (PRP) was produced from each mare’s blood (100 mL). The collected PRP was subsequently frozen at -80°C to obtain platelet lysate.
- During the untreated cycles, the mares were inseminated with frozen-thawed semen 36 hours after inducing ovulation. In treated cycles, the PL was thawed and infused into the uterus, with artificial insemination occurring 24 hours later.
Findings and Analysis
- The effect of the PL treatment was evaluated using various measures such as neutrophils count in uterine cytology, uterine fluid accumulation, and uterine edema, which were carefully examined via optical microscopy and ultrasonography techniques.
- Observations from treated cycles revealed a significant decrease in cytology score, fluid accumulation, and edema.
- The cytology score was reduced to an average of 1.3 (PL) from the average untreated score of 2.0 (Ctr).
- Similarly, fluid accumulation decreased from an average of 342.7 mm untreated to 79.5 mm with PL treatment.
- The edema score also saw a reduction from 2.3 average in untreated cycles to 1.8 with PL treatment.
- However, the pregnancy rate in PL-treated cycles (3 out of 12) and control cycles (2 out of 14) did not show a significant difference.
Implications of the Research
- The decrease in clinical signs of uterine edema, inflammatory uterine fluid accumulation, and migration of neutrophils to the uterus observed in PL cycles suggest that PL treatment can effectively minimize the inflammatory uterine response in the mares susceptible to PMIE.
- Nonetheless, the fact that pregnancy rates between treated and untreated cycles were statistically similar indicates that further studies on improving fertility outcomes are required.
Cite This Article
APA
Colombo I, Mislei B, Mari G, Iacono E, Merlo B.
(2021).
Effect of platelet lysate on uterine response of mares susceptible to persistent mating-induced endometritis.
Theriogenology, 179, 204-210.
https://doi.org/10.1016/j.theriogenology.2021.12.001 Publication
Researcher Affiliations
- DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia, BO, Italy. Electronic address: ilaria.colombo3@unibo.it.
- AUB INFA, National Institute of Artificial Insemination, University of Bologna, Via Gandolfi 16, 40057, Granarolo dell'Emilia, BO, Italy.
- DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia, BO, Italy; AUB INFA, National Institute of Artificial Insemination, University of Bologna, Via Gandolfi 16, 40057, Granarolo dell'Emilia, BO, Italy.
- DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia, BO, Italy.
- DIMEVET, Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia, BO, Italy.
MeSH Terms
- Animals
- Endometritis / prevention & control
- Endometritis / veterinary
- Female
- Horse Diseases
- Horses
- Insemination, Artificial / veterinary
- Pregnancy
- Reproduction
- Uterus
Conflict of Interest Statement
Declaration of competing interest None.
Citations
This article has been cited 3 times.- Yousef MS, Akthar I, Ma D, Haneda S, Kusama K, Shimada M, Imakawa K, Miyamoto A. Platelet secretions exert anti-inflammatory effects in vitro on neutrophils and uterine epithelia in cattle: a possible role in amplifying the uterine immune network toward pregnancy. Front Immunol 2025;16:1560996.
- Lange-Consiglio A, Gaspari G, Funghi F, Capra E, Cretich M, Frigerio R, Bosi G, Cremonesi F. Amniotic Mesenchymal-Derived Extracellular Vesicles and Their Role in the Prevention of Persistent Post-Breeding Induced Endometritis. Int J Mol Sci 2023 Mar 8;24(6).
- Wu ZS, Luo HL, Chuang YC, Lee WC, Wang HJ, Chancellor MB. Platelet Lysate Therapy Attenuates Hypoxia Induced Apoptosis in Human Uroepithelial SV-HUC-1 Cells through Regulating the Oxidative Stress and Mitochondrial-Mediated Intrinsic Apoptotic Pathway. Biomedicines 2023 Mar 17;11(3).
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