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Home / Equine Research Bank / Infect Immun / Effect of prednisolone on the leukocyte counts of ponies and...
Infection and immunity1975; 11(2); 252-256; doi: 10.1128/iai.11.2.252-256.1975

Effect of prednisolone on the leukocyte counts of ponies and on the reactivity of lymphocytes in vitro and in vivo.

Abstract: Treatment of ponies with a single dose of prednisolone markedly reduced the number of blood lymphocytes. A decrease of the number of eosinophils was also observed. In contrast, the number of neutrophils significantly increased. These profound changes were temporary and returned to the pretreatment level within 48 h. The number of monocytes did not show any of the significant changes post-prednisolone treatment. The reactivity of the blood lymphocytes of these ponies, in vitro, to stimulation with phytohemagglutinin (PHA) or streptokinase-strepto-dornase (SK-SD) was measured by incorporation of (3-H)thymidine by deoxyribonucleic acid of lymphocytes. The ponies' blood lymphocytes responded very well to PHA stimulation. The incorporation of (3-H)thymidine into deoxyribonucleic acid of the PHA-stimulated lymphocytes was 14 times greater than incorporation of the nonstimulated lymphocytes. SK-SD-stimulated lymphocytes incorporated only three times more (3-H)thymidine than nonstimulated lymphocytes. There was no significant difference in stimulation of lymphocytes with PHA or SK-SD before or after the prednisolone treatment. However, a significant decrease in the dermal response to the homologous antigen after this treatment in sensitive ponies was observed.
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Publication Date: 1975-02-01 PubMed ID: 1112615PubMed Central: PMC415053DOI: 10.1128/iai.11.2.252-256.1975Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores how the treatment of ponies with a single dose of prednisolone, a steroid medication, affects the number and reactivity of different types of white blood cells, specifically lymphocytes.

Impact on Leukocyte Counts

  • The researchers observed that after treating the ponies with prednisolone, there was a notable decrease in blood lymphocytes and eosinophils, with a concurrent increase in neutrophils.
  • However, these changes were temporary as counts returned to the pretreatment level within a period of 48 hours.
  • The count of monocytes remained stable – no significant changes were observed post-prednisolone treatment.

Impact on Lymphocyte Reactivity

  • Lymphocyte reactivity was tested both in vitro (outside a living organism) and in vivo (inside a living organism), with the use of stimulants phytohemagglutinin (PHA) and streptokinase-streptodornase (SK-SD).
  • The reactivity was measured by incorporating radioactive thymidine into the DNA of the lymphocytes and tracing its activity.
  • The authors observed that lymphocytes reacted more intensely to PHA stimulation, incorporating 14 times more radioactive thymidine than non-stimulated cells.
  • Lymphocytes showed a less intense response to SK-SD stimulation, incorporating only three times more radioactive thymidine than non-stimulated cells.
  • Interestingly, the team experienced no significant difference in lymphocyte reactivity to the two stimulants both before and after the administration of prednisolone.

Changes in Dermal Response to Antigen

  • The research noted a significant decrease in the skin’s (dermal) response to the ponies’ own (homologous) antigens post-prednisolone treatment in ponies that were originally sensitive.

In conclusion, the study provides valuable insights into how prednisolone temporarily affects the counts and reactivity of different white blood cells in ponies, and how it potentially influences their sensitivity to their own antigens. This could hold potential clinical implications for the use of prednisolone in veterinary medicine, particularly concerning immune function and reactivity.

Cite This Article

APA
Targowski SP. (1975). Effect of prednisolone on the leukocyte counts of ponies and on the reactivity of lymphocytes in vitro and in vivo. Infect Immun, 11(2), 252-256. https://doi.org/10.1128/iai.11.2.252-256.1975

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 11
Issue: 2
Pages: 252-256

Researcher Affiliations

Targowski, S P

    MeSH Terms

    • Animals
    • Antigens
    • Cell Separation
    • Cells, Cultured
    • Horses
    • Lectins
    • Leukocyte Count
    • Leukocytes / drug effects
    • Lymphocyte Activation / drug effects
    • Lymphocytes / drug effects
    • Prednisolone / pharmacology
    • Skin / drug effects
    • Skin Tests
    • Streptodornase and Streptokinase
    • Thymidine / metabolism
    • Tritium

    References

    This article includes 17 references
    1. BOVORNKITTI S, KANGSADAL P, SATHIRAPAT P, OONSOMBATTI P. Reversion and reconversion rate of tuberculin skin reactions in correction with the use of prednisone.. Dis Chest 1960 Jul;38:51-5.
      pubmed: 13803423doi: 10.1378/chest.38.1.51google scholar: lookup
    2. Yu DT, Clements PJ, Paulus HE, Peter JB, Levy J, Barnett EV. Human lymphocyte subpopulations. Effect of corticosteroids.. J Clin Invest 1974 Feb;53(2):565-71.
      pubmed: 11344571doi: 10.1172/JCI107591google scholar: lookup
    3. Paape MJ, Carroll DW, Kral AJ, Miller RH, Desjardins C. Corticosteroids, circulating leukocytes, and erythrocytes in cattle: diurnal changes and effects of bacteriologic status, stage of lactation, and milk yield on response to adrenocorticotropin.. Am J Vet Res 1974 Mar;35(3):355-62.
      pubmed: 4362211
    4. Osbaldiston GW, Johnson JH. Effect of ACTH and selected glucocorticoids on circulating blood cells in horses.. J Am Vet Med Assoc 1972 Jul;161(1):53-6.
      pubmed: 4338560
    5. Davies DH, Carmichael LE. Role of cell-mediated immunity in the recovery of cattle from primary and recurrent infections with infectious bovine rhinotracheitis virus.. Infect Immun 1973 Oct;8(4):510-8.
      pubmed: 4355136doi: 10.1128/iai.8.4.510-518.1973google scholar: lookup
    6. Weston WL, Mandel MJ, Krueger GG, Claman HN. Differential suppressive effect of hydrocortisone on lymphocytes and mononuclear macrophages in delayed hypersensitivity of guinea pigs.. J Invest Dermatol 1972 Oct;59(4):345-8.
      pubmed: 4631552doi: 10.1111/1523-1747.ep12627402google scholar: lookup
    7. Mishra SK, Sandhu RS, Randhawa HS, Damodaran VN, Abraham S. Effect of cortisone administration on experimental nocardiosis.. Infect Immun 1973 Feb;7(2):123-9.
      pubmed: 4572606doi: 10.1128/iai.7.2.123-129.1973google scholar: lookup
    8. Rosenstreich DL, Rosenthal AS. Peritoneal exudate lymphocyte. 3. Dissociation of antigen-reactive lymphocytes from antigen-binding cells in a T lymphocyte enriched population in the guinea pig.. J Immunol 1974 Mar;112(3):1085-93.
      pubmed: 4544176
    9. Shortman K, Byrd WJ, Cerottini JC, Brunner KT. Characterisation and separation of mouse lymphocyte subpopulations responding to phytohemagglutinin and pokeweed mitogens.. Cell Immunol 1973 Jan;6(1):25-40.
      pubmed: 4539718doi: 10.1016/0008-8749(73)90003-8google scholar: lookup
    10. Hagen C, Froland A. Analysis of the variation in lymphocyte response to PHA in normal subjects.. Acta Pathol Microbiol Scand B Microbiol Immunol 1973 Apr;81(2):253-8.
      pubmed: 4520380doi: 10.1111/j.1699-0463.1973.tb00219.xgoogle scholar: lookup
    11. Fauci AS, Dale DC. The effect of in vivo hydrocortisone on subpopulations of human lymphocytes.. J Clin Invest 1974 Jan;53(1):240-6.
      pubmed: 4808638doi: 10.1172/JCI107544google scholar: lookup
    12. Balow JE, Rosenthal AS. Glucocorticoid suppression of macrophage migration inhibitory factor.. J Exp Med 1973 Apr 1;137(4):1031-41.
      pubmed: 4693151doi: 10.1084/jem.137.4.1031google scholar: lookup
    13. Weston WL, Claman HN, Krueger GG. Site of action of cortisol in cellular immunity.. J Immunol 1973 Mar;110(3):880-3.
      pubmed: 4688923
    14. Boldt S, Skinner AM, Kornfeld S. Studies of two subpopulations of human lymphocytes differing in responsiveness to concanavalin A.. J Clin Invest 1972 Dec;51(12):3225-34.
      pubmed: 4640956doi: 10.1172/JCI107149google scholar: lookup
    15. Graf PA, Mather F. A statistical model for the evaluation of antigen-stimulated blastogenic transformation as measured by scintillation spectrometry.. Cell Immunol 1972 Jun;4(2):143-51.
      pubmed: 5028164doi: 10.1016/0008-8749(72)90013-5google scholar: lookup
    16. Claman HN. Corticosteroids and lymphoid cells.. N Engl J Med 1972 Aug 24;287(8):388-97.
      pubmed: 5043524doi: 10.1056/NEJM197208242870806google scholar: lookup
    17. Linares LI, Friedman L. Experimental paracoccidioidomycosis in mice.. Infect Immun 1972 May;5(5):681-7.
      pubmed: 4637603doi: 10.1128/iai.5.5.681-687.1972google scholar: lookup

    Citations

    This article has been cited 4 times.
    1. Whiteman JP, Harlow HJ, Durner GM, Regehr EV, Amstrup SC, Pagano AM, Ben-David M. The acute physiological response of polar bears to helicopter capture. J Wildl Manage 2022 Jul;86(5):e22238.
      doi: 10.1002/jwmg.22238pubmed: 35915725google scholar: lookup
    2. Mainguy-Seers S, Lavoie JP. Glucocorticoid treatment in horses with asthma: A narrative review. J Vet Intern Med 2021 Jul;35(4):2045-2057.
      doi: 10.1111/jvim.16189pubmed: 34085342google scholar: lookup
    3. Targowski SP. Serum immunoglobulin, dermal response, and lymphocyte transformation studies in horses with chronic diarrhea. Infect Immun 1975 Jul;12(1):48-54.
      doi: 10.1128/iai.12.1.48-54.1975pubmed: 806535google scholar: lookup
    4. de la Cuesta-Torrado M, Vitale V, Velloso Alvarez A, Neira-Egea P, Diss C, Cuervo-Arango J. The Effect of Vaccination Status on Total Lymphocyte Count in Horses Affected by Equine Herpes Virus-1 Myeloencephalopathy. Animals (Basel) 2025 Apr 1;15(7).
      doi: 10.3390/ani15071019pubmed: 40218411google scholar: lookup
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