Effect of prostaglandin F2alpha on ovarian, adrenal, and pituitary hormones and on luteal blood flow in mares.
Abstract: The effect of a single injection of prostaglandin F2alpha (PGF) during mid-diestrus on systemic concentrations of progesterone, LH, FSH, estradiol, and cortisol and on blood flow to the corpus luteum was studied in 10 controls and 10 PGF-treated mares. Blood flow was assessed by estimating the percentage of corpus luteum with color-Doppler signals of blood flow during real-time scanning of the entire structure and by the diameter of the vascular pedicle near its attachment to the ovary. Treatment was done 8 days after ovulation and 0 h was immediately before the treatment. Examinations and collection of blood samples were done at 0 h, every 5 min until 1h, and then at 1.5, 2, 4, 8, 12, 24, 48, and 72 h. The concentrations of estradiol did not change, but progesterone, LH, FSH, and cortisol increased significantly within 5 min. Concentrations of LH and FSH in the PGF group remained elevated until a temporarily lower concentration at 8 or 4h, respectively, rebounded to 12h, and then slowly decreased. Cortisol remained elevated, until a decrease between 1 and 4h. Progesterone in the PGF group increased significantly until 10 min after 0 h and then decreased by 40 min to below the concentrations in controls. Within the PGF group, progesterone decreased significantly by 45 min to below the concentrations at 0 h. The values for each of the two indicators of blood flow did not differ significantly between the PGF and control groups until a decrease at 24h in the PGF group. Results did not support the hypothesis that the immediate transient post-PGF increase in progesterone was associated with an increase in luteal blood flow. Luteolysis, as indicated by decreasing progesterone, began well before the beginning of a decrease in luteal blood flow.
Publication Date: 2006-04-27 PubMed ID: 16698221DOI: 10.1016/j.domaniend.2006.04.006Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates the effect of a single prostaglandin F2alpha (PGF) injection on mare’s progesterone, LH, FSH, estradiol, cortisol hormones and blood flow to the corpus luteum. It found that the immediate increase in progesterone following the injection was not connected to an increase in blood flow to the luteum and the decrease in progesterone that signifies luteolysis happens before a decrease in blood flow is detected.
Methodology and Treatment Administered
- The study involved 10 control mares and 10 mares treated with prostaglandin F2alpha (PGF).
- The prostaglandin was administered 8 days after ovulation and blood samples were collected at different time intervals starting from immediately prior to the treatment.
- Following the injections, examinations of blood flow to the corpus luteum were performed using color-Doppler imaging.
Findings and Changes in Hormone Levels
- The concentrations of estradiol did not change following the PGF injection.
- Progesterone, LH, FSH, and cortisol levels increased significantly within just 5 minutes of the PGF injection.
- LH and FSH concentrations in the PGF group remained high until they temporarily fell at about 8 or 4 hours respectively, before rebounding at 12 hours and then slowly dropping.
- Cortisol levels also remained high, until a decrease between the 1st and 4th hours. Progesterone in the PGF group rose significantly until 10 minutes after the injection but had dropped to below the control levels by the 40-minute mark.
Impact on Luteal Blood Flow and Luteolysis
- There was no significant change in the indicators of blood flow to the luteum between the PGF and control groups until a decrease at 24 hours in the PGF group.
- The results did not support the hypothesis that the immediate, transient, post-PGF increase in progesterone was associated with an increase in blood flow to the luteum.
- The decrease in progesterone levels, signaling the start of luteolysis, was observed well before the decrease in blood flow to the luteum.
Cite This Article
APA
Ginther OJ, Gastal EL, Gastal MO, Beg MA.
(2006).
Effect of prostaglandin F2alpha on ovarian, adrenal, and pituitary hormones and on luteal blood flow in mares.
Domest Anim Endocrinol, 32(4), 315-328.
https://doi.org/10.1016/j.domaniend.2006.04.006 Publication
Researcher Affiliations
- Eutheria Foundation, Cross Plains, WI 53528, United States. ginther@svm.vetmed.wisc.edu
MeSH Terms
- Animals
- Corpus Luteum / blood supply
- Corpus Luteum / growth & development
- Diestrus / blood
- Dinoprost / physiology
- Estradiol / blood
- Female
- Follicle Stimulating Hormone / blood
- Horses
- Hydrocortisone / blood
- Luteinizing Hormone / blood
- Luteolysis / blood
- Progesterone / blood
- Regional Blood Flow
Citations
This article has been cited 4 times.- Requena F, Campos MJAPM, Martínez Marín AL, Camacho R, Giráldez-Pérez RM, Agüera EI. Assessment of Age Effects on Ovarian Hemodynamics Using Doppler Ultrasound and Progesterone Concentrations in Cycling Spanish Purebred Mares. Animals (Basel) 2021 Aug 8;11(8).
- Kaps M, Okada CTC, Gautier CM, Aurich J, Aurich C. Deslorelin Slow-Release Implants Delay Ovulation and Increase Plasma AMH Concentration and Small Antral Follicles in Haflinger Mares. Animals (Basel) 2021 May 28;11(6).
- Ahmed K, LaPierre MP, Gasser E, Denzler R, Yang Y, Rülicke T, Kero J, Latreille M, Stoffel M. Loss of microRNA-7a2 induces hypogonadotropic hypogonadism and infertility. J Clin Invest 2017 Mar 1;127(3):1061-1074.
- Araujo RR, Ginther OJ, Ferreira JC, Palhão MM, Beg MA, Wiltbank MC. Role of follicular estradiol-17beta in timing of luteolysis in heifers. Biol Reprod 2009 Aug;81(2):426-37.
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