Effect of seminal plasma concentration and various extenders on postthaw motility and glass wool-Sephadex filtration of cryopreserved stallion semen.

The research focuses on the impact of seminal plasma concentration and different extenders on the post-thaw movement and glass wool- Sephadex (GWS) filtration of frozen stallion semen. The findings revealed that using Kenney extender with a high dilution ratio and maintaining 5% seminal plasma concentration can significantly improve the post-thaw quality of frozen stallion semen.

Research Methodology

• The semen samples were harvested from seven stallions with a minimum of three ejaculates taken from each. All stallions used for this study consistently produced semen with poor post-thaw quality.
• Four separate experiments were designed to assess the post-thaw quality of this frozen stallion semen. These experiments compared the effects of seminal plasma concentration, semen extenders, and their dilution rates.
• The comparisons involved two types of extender; Kenney extender and glucose-EDTA extender. Different dilution rates were used to analyze the effect of seminal plasma concentrations on semen quality.

Research Findings

• The use of the Kenney extender as the centrifugation extender notably improved post-thaw motility and GWS filtration results when compared with the glucose-EDTA extender.
• A dilution ratio of 1:3 was found to be significantly more effective in promoting motility and improving GWS filtration than the 1:1 dilution ratio.
• Including seminal plasma at a concentration of 5% in the frozen semen was found to yield a significantly high number of spermatozoa after GWS filtration. This was in comparison with complete removal of seminal plasma or inclusion of seminal plasma at a 25% concentration.
• Semen samples with poor post-thaw quality showed a notable improvement in post-thaw quality in relation to motility and GWS filtration. This improvement was observed when seminal plasma at a 5% concentration was used for the freezing process, as compared to the use of seminal plasma at a 25% concentration.

Conclusion

• The use of the Kenney extender with a high dilution ratio immediately after collection, as well as inclusion of a 5% seminal plasma concentration can result in a great improvement in post-thaw quality of frozen stallion semen.