Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses.
Abstract: The aim of this study was to determine the effect of single layer centrifugation (SLC) using Androcoll-E-Large on donkey sperm quality parameters after 24 h of cool-storage. Ejaculates were collected from Andalusian donkeys and then cooled at 5°C. SLC was carried out after 24 h of cool-storage using Androcoll-E-Large. In the first experiment, all sperm parameters assessed (total and progressive sperm motility, viability, sperm morphology and sperm kinematics VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) were statistically compared between semen samples processed or not with Androcoll-E-Large. Significant differences (P<0.05) were found between SLC-selected and unselected semen samples for all parameters assessed, obtaining better results after SLC. In the second experiment, semen samples were classified in two groups according to their sperm progressive motility (PM) before SLC. Then, the increments obtained in semen quality parameters after SLC were compared between groups. No significant differences were found between groups, indicating that SLC improved the sperm quality parameters of entire set of semen samples processed with independence to their original PM. In conclusion, SLC with Androcoll-E-Large can be used in donkeys, increasing the sperm quality of cooled-stored donkey semen doses after 24 h of cool storage.
Publication Date: 2013-11-28 PubMed ID: 24284302DOI: 10.1017/S1751731113002097Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study explores the impact of applying single layer centrifugation (SLC) technique using a matter called Androcoll-E-Large on the quality of donkey sperm stored in a cool environment. The results found that this method led to improvements in the parameters that define sperm quality.
Objective of the Research
- The primary aim of this research was to find out the effect of using single layer centrifugation (SLC) in combination with Androcoll-E-Large on the quality of donkey sperm when stored in a cooled state for 24 hours.
Methods of the Research
- Ejaculates were gathered from Andalusian donkeys and subsequently chilled at 5°C.
- SLC was performed after the samples were cold-stored for 24 hours with the use of Androcoll-E-Large.
- Researchers then compared a variety of sperm parameters such as motility, viability, morphology, and sperm kinematics in samples that were processed with Androcoll-E-Large and those that were not, in an initial experiment.
- In the following procedure, sperm samples were sorted into two groups based on their progressive motility (PM) prior to SLC. Then, the enhancements in sperm quality parameters post-SLC were compared between the groups.
Results of the Research
- The study found significant variances between SLC-selected and non-selected semen samples for all tested parameters, with better results shown post-SLC.
- No significant differences were observed when comparing the enhancements in sperm quality parameters after SLC between the groups, which suggests that SLC consistently improved the sperm quality parameters across all semen samples, regardless of their initial PM.
Conclusion of the Research
- The final conclusion drawn from this research is that SLC, alongside Androcoll-E-Large, can be effectively used in donkeys, as it tends to boost the quality of cooled-stored donkey semen samples even after 24 hours of cool storage.
Cite This Article
APA
Ortiz I, Dorado J, Ramírez L, Morrell JM, Acha D, Urbano M, Gálvez MJ, Carrasco JJ, Gómez-Arrones V, Calero-Carretero R, Hidalgo M.
(2013).
Effect of single layer centrifugation using Androcoll-E-Large on the sperm quality parameters of cooled-stored donkey semen doses.
Animal, 8(2), 308-315.
https://doi.org/10.1017/S1751731113002097 Publication
Researcher Affiliations
- 1 Animal Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain.
- 1 Animal Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain.
- 1 Animal Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain.
- 2 Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, SE-75007, Uppsala, Sweden.
- 1 Animal Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain.
- 1 Animal Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain.
- 1 Animal Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain.
- 3 Equine Center for Assisted Reproduction Services, CENSYRA-Extremadura Government, 06007 Badajoz, Spain.
- 3 Equine Center for Assisted Reproduction Services, CENSYRA-Extremadura Government, 06007 Badajoz, Spain.
- 3 Equine Center for Assisted Reproduction Services, CENSYRA-Extremadura Government, 06007 Badajoz, Spain.
- 1 Animal Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, 14071 Cordoba, Spain.
MeSH Terms
- Analysis of Variance
- Animals
- Biomechanical Phenomena
- Cell Survival / physiology
- Centrifugation / methods
- Cold Temperature
- Equidae / physiology
- Male
- Sperm Motility / physiology
- Spermatozoa / cytology
- Spermatozoa / physiology
- Tissue Preservation / methods
- Tissue Preservation / veterinary
Citations
This article has been cited 6 times.- Martins-Bessa A, Quaresma M, Leiva B, Calado A, Navas González FJ. Bayesian Linear Regression Modelling for Sperm Quality Parameters Using Age, Body Weight, Testicular Morphometry, and Combined Biometric Indices in Donkeys. Animals (Basel) 2021 Jan 13;11(1).
- Ortiz I, Dorado J, Morrell JM, Diaz-Jimenez MA, Pereira B, Consuegra C, Hidalgo M. Comparison of sperm selection techniques in donkeys: motile subpopulations from a practical point of view. Anim Reprod 2019 Oct 23;16(2):282-289.
- Papas M, Catalán J, Recuero S, Morrell JM, Yeste M, Miró J. Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils. Animals (Basel) 2020 Nov 16;10(11).
- Perrier JP, Sellem E, Prézelin A, Gasselin M, Jouneau L, Piumi F, Al Adhami H, Weber M, Fritz S, Boichard D, Le Danvic C, Schibler L, Jammes H, Kiefer H. A multi-scale analysis of bull sperm methylome revealed both species peculiarities and conserved tissue-specific features. BMC Genomics 2018 May 29;19(1):404.
- Al-Kass Z, Spergser J, Aurich C, Kuhl J, Schmidt K, Johannisson A, Morrell JM. Sperm Quality during Storage Is Not Affected by the Presence of Antibiotics in EquiPlus Semen Extender but Is Improved by Single Layer Centrifugation. Antibiotics (Basel) 2017 Dec 21;7(1).
- Ortiz I, Dorado J, Morrell J, Gosálvez J, Crespo F, Jiménez JM, Hidalgo M. New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models. J Anim Sci Biotechnol 2017;8:23.
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