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Discover nano2024; 19(1); 80; doi: 10.1186/s11671-024-04026-4

Effect of storage conditions on the quality of equine and canine mesenchymal stem cell derived nanoparticles including extracellular vesicles for research and therapy.

Abstract: Nanoparticles including extracellular vesicles derived from mesenchymal stem cells are of increasing interest for research and clinical use in regenerative medicine. Extracellular vesicles (EVs), including also previously named exosomes, provide a promising cell-free tool for therapeutic applications, which is probably a safer approach to achieve sufficient healing. Storage of EVs may be necessary for clinical applications as well as for further experiments, as the preparation is sometimes laborious and larger quantities tend to be gained. For this purpose, nanoparticles were obtained from mesenchymal stem cells from adipose tissue (AdMSC) of horses and dogs. The EVs were then stored for 7 days under different conditions (- 20 °C, 4 °C, 37 °C) and with the addition of various additives (5 mM EDTA, 25-250 µM trehalose). Afterwards, the size and number of EVs was determined using the nano tracking analyzing method. With our investigations, we were able to show that storage of EVs for up to 7 days at 4 °C does not require the addition of supplements. For the other storage conditions, in particular freezing and storage at room temperature, the addition of EDTA was found to be suitable for preventing aggregation of the particles. Contrary to previous publications, trehalose seems not to be a suitable cryoprotectant for AdMSC-derived EVs. The data are useful for processing and storage of isolated EVs for further experiments or clinical approaches in veterinary medicine.
© 2024. The Author(s).
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Publication Date: 2024-05-03 PubMed ID: 38700790PubMed Central: 4002895DOI: 10.1186/s11671-024-04026-4Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study looks into the effect of various storage conditions on the quality of nanoparticles, specifically extracellular vesicles (EVs), derived from mesenchymal stem cells found in the adipose tissue of horses and dogs. The researchers found that EVs can be stored at 4°C for up to a week without additives, whereas preserving these particles at other temperatures may require the addition of EDTA to prevent clumping. They also determined that contrary to previous studies, trehalose does not function well as a cryoprotectant for these EVs.

Objective of the Research

  • The primary goal of this research was to investigate how different storage conditions impact the quality of nanoparticles that include extracellular vesicles (EVs) sourced from mesenchymal stem cells in horses and dogs.
  • The researchers evaluated the feasibility of storing EVs under various conditions, testing different temperatures and additives.

Methodology

  • Extracellular vesicles were obtained from mesenchymal stem cells in the adipose tissue (fat cells) of horses and dogs. This type of stem cell is known as an adipose tissue-derived mesenchymal stem cell (AdMSC).
  • The EVs were stored under different conditions for seven days. These conditions included varied temperatures of -20°C, 4°C, and 37°C.
  • They also tested the addition of various additives i.e., 5mM EDTA and 25-250 µM trehalose, to see their impact on EVs preservation.
  • Lastly, the size and number of EVs were determined using a method called nano tracking analysis, which allows for the visualization and tracking of nanoparticles.

Findings and Implications

  • The research concluded that EVs can be stored safely at 4°C for up to 7 days without the need for any additives.
  • For other storage conditions, particularly freezing (at -20°C) and at ambient temperature (around 37°C), the addition of EDTA was found to be beneficial in preventing aggregation or clumping of the particles.
  • Contrary to previous studies, the scientists determined that trehalose doesn’t seem to be a suitable cryoprotectant for the AdMSC-derived EVs. A cryoprotectant helps protect biological tissue from freezing damage.
  • The findings of this study are relevant for both research and clinical contexts in veterinary medicine, especially in the field of regenerative medicine. It offers valuable insights on how stem cell-derived EVs should be stored for future use in both laboratory experiments and therapeutic applications.

Cite This Article

APA
Klymiuk MC, Balz N, Elashry MI, Wenisch S, Arnhold S. (2024). Effect of storage conditions on the quality of equine and canine mesenchymal stem cell derived nanoparticles including extracellular vesicles for research and therapy. Discov Nano, 19(1), 80. https://doi.org/10.1186/s11671-024-04026-4

Publication

Discover nano
ISSN: 2731-9229
NlmUniqueID: 9918540788706676
Country: Switzerland
Language: English
Volume: 19
Issue: 1
Pages: 80

Researcher Affiliations

Klymiuk, Michele Christian
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany. michele.klymiuk@vetmed.uni-giessen.de.
Balz, Natalie
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Elashry, Mohamed I
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Wenisch, Sabine
  • Clinic of Small Animals, c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.
Arnhold, Stefan
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35392, Giessen, Germany.

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Citations

This article has been cited 2 times.
  1. Cheng S, Ma Y, Huang F, Luo R, Han L, He L, Yuan ZX. Mesenchymal Stem Cell-Derived Exosomes for Ocular Diseases: Therapeutic Mechanisms and Clinical Perspectives. Int J Nanomedicine 2025;20:14521-14550.
    doi: 10.2147/IJN.S555771pubmed: 41383281google scholar: lookup
  2. Aldarmahi AA, Khanday S, Taher ES, Abdeen A, Atia GA, Mohammed DA, Nasr DS, Albarakati RG, Zaghamir DE, Hetta HF, Atwa AM, Abass KS, Elmorsy EM, Alshambky A, El-Sakhawy MA, El-Far A, Attia SS. From biomolecules to breakthroughs: exosomes as next-generation theranostics in female infertility. Front Cell Dev Biol 2025;13:1605174.
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